ENSG00000225190

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000430334 ENSG00000225190 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHM1 protein_coding protein_coding 9.815118 14.62761 5.250662 1.272147 0.4122432 -1.476364 5.2393712 8.0107648 3.4624629 1.0783831 0.12930855 -1.20778052 0.5507042 0.54500000 0.6644 0.11940000 3.193121e-01 1.011033e-07 FALSE TRUE
ENST00000580404 ENSG00000225190 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHM1 protein_coding processed_transcript 9.815118 14.62761 5.250662 1.272147 0.4122432 -1.476364 1.1734361 3.1728883 0.0000000 0.8577920 0.00000000 -8.31419273 0.0902750 0.22710000 0.0000 -0.22710000 1.011033e-07 1.011033e-07 FALSE TRUE
ENST00000581448 ENSG00000225190 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHM1 protein_coding nonsense_mediated_decay 9.815118 14.62761 5.250662 1.272147 0.4122432 -1.476364 0.8596549 0.7901432 0.7757644 0.1015865 0.04463143 -0.02616144 0.0994500 0.05506667 0.1484 0.09333333 3.348201e-04 1.011033e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000225190 E001 1.6650287 0.0084350887 2.990481e-02 7.177212e-02 17 45435900 45435902 3 - 0.620 0.256 -1.978
ENSG00000225190 E002 2.1433194 0.0066097148 1.407305e-01 2.490143e-01 17 45435903 45435903 1 - 0.620 0.381 -1.171
ENSG00000225190 E003 374.3941790 0.0036446119 1.683135e-09 2.391008e-08 17 45435904 45437470 1567 - 2.328 2.548 0.732
ENSG00000225190 E004 45.3799619 0.0005131890 2.229872e-02 5.629264e-02 17 45437471 45437548 78 - 1.470 1.629 0.545
ENSG00000225190 E005 40.0494726 0.0043234719 1.675736e-02 4.440106e-02 17 45437549 45437629 81 - 1.393 1.586 0.661
ENSG00000225190 E006 118.1131208 0.0073275334 2.441914e-04 1.150894e-03 17 45437630 45437915 286 - 1.825 2.052 0.762
ENSG00000225190 E007 60.9229023 0.0032349766 3.482287e-04 1.572982e-03 17 45437916 45437969 54 - 1.528 1.768 0.816
ENSG00000225190 E008 61.3579588 0.0004353002 4.793396e-02 1.055126e-01 17 45439477 45439513 37 - 1.625 1.747 0.415
ENSG00000225190 E009 60.3429780 0.0004654120 2.686375e-01 4.071178e-01 17 45439514 45439545 32 - 1.656 1.731 0.254
ENSG00000225190 E010 53.2711407 0.0004408942 3.580914e-01 5.024727e-01 17 45439546 45439577 32 - 1.608 1.676 0.230
ENSG00000225190 E011 45.1961238 0.0044389250 5.462191e-01 6.764299e-01 17 45439578 45439614 37 - 1.548 1.607 0.202
ENSG00000225190 E012 40.9246567 0.0061594188 8.365467e-01 8.977990e-01 17 45439615 45439634 20 - 1.548 1.552 0.015
ENSG00000225190 E013 0.6966155 0.0166724610 9.602914e-01 9.789945e-01 17 45440027 45440162 136 - 0.184 0.205 0.192
ENSG00000225190 E014 53.7757451 0.0011954655 1.609331e-01 2.763398e-01 17 45440163 45440226 64 - 1.591 1.689 0.333
ENSG00000225190 E015 3.0027088 0.2568707332 5.497242e-01 6.794293e-01 17 45440227 45440373 147 - 0.411 0.575 0.806
ENSG00000225190 E016 2.3175326 0.0067264529 8.231768e-02 1.631703e-01 17 45440837 45441059 223 - 0.184 0.533 2.192
ENSG00000225190 E017 79.2073723 0.0025606949 8.072912e-01 8.775772e-01 17 45445470 45445663 194 - 1.832 1.837 0.016
ENSG00000225190 E018 2.3637204 0.0064637985 6.912768e-02 1.419301e-01 17 45446129 45446273 145 - 0.671 0.381 -1.393
ENSG00000225190 E019 1.6532736 0.0091653873 1.251734e-01 2.273912e-01 17 45447912 45448112 201 - 0.561 0.302 -1.393
ENSG00000225190 E020 50.0563071 0.0004564223 3.643948e-01 5.087627e-01 17 45450618 45450763 146 - 1.661 1.627 -0.116
ENSG00000225190 E021 7.4637019 0.0401357348 5.110340e-01 6.461451e-01 17 45452938 45453354 417 - 0.924 0.846 -0.296
ENSG00000225190 E022 115.1750775 0.0007847508 8.935961e-01 9.359401e-01 17 45453355 45453973 619 - 1.970 1.993 0.076
ENSG00000225190 E023 96.3883012 0.0043040547 7.488610e-02 1.513484e-01 17 45453974 45454272 299 - 1.965 1.898 -0.226
ENSG00000225190 E024 1.9938934 0.0416923437 2.966721e-01 4.379345e-01 17 45454273 45454771 499 - 0.561 0.381 -0.909
ENSG00000225190 E025 87.5914062 0.0003915882 6.884740e-02 1.414620e-01 17 45458169 45458439 271 - 1.918 1.858 -0.201
ENSG00000225190 E026 0.0000000       17 45459383 45460432 1050 -      
ENSG00000225190 E027 19.9306978 0.0039019396 9.347155e-01 9.628780e-01 17 45468209 45468245 37 - 1.239 1.248 0.032
ENSG00000225190 E028 75.4804041 0.0003387068 4.108244e-02 9.303621e-02 17 45468246 45468593 348 - 1.865 1.790 -0.252
ENSG00000225190 E029 48.1291431 0.0004590971 8.640451e-07 7.276114e-06 17 45475100 45475210 111 - 1.790 1.546 -0.829
ENSG00000225190 E030 56.7814534 0.0005086223 2.665645e-07 2.493204e-06 17 45475211 45475362 152 - 1.852 1.615 -0.803
ENSG00000225190 E031 17.9278919 0.0012329415 2.029158e-03 7.316949e-03 17 45475363 45475365 3 - 1.374 1.129 -0.864
ENSG00000225190 E032 44.8626596 0.0005385047 8.667937e-02 1.700903e-01 17 45475366 45475435 70 - 1.646 1.563 -0.282
ENSG00000225190 E033 58.4453669 0.0004140972 2.113737e-01 3.401091e-01 17 45475436 45475596 161 - 1.735 1.687 -0.162
ENSG00000225190 E034 45.4830284 0.0101662887 1.478817e-01 2.588035e-01 17 45475597 45475695 99 - 1.656 1.571 -0.290
ENSG00000225190 E035 38.1818924 0.0044279522 2.775773e-05 1.663909e-04 17 45475696 45475726 31 - 1.699 1.442 -0.880
ENSG00000225190 E036 5.1113599 0.0041515312 1.245063e-01 2.264095e-01 17 45475888 45476073 186 - 0.865 0.664 -0.807
ENSG00000225190 E037 7.2108704 0.0026890861 3.092713e-01 4.515410e-01 17 45477074 45477804 731 - 0.923 0.808 -0.444
ENSG00000225190 E038 3.7602623 0.0329274419 1.326792e-02 3.654292e-02 17 45477805 45477899 95 - 0.865 0.481 -1.641
ENSG00000225190 E039 67.3218457 0.0070824702 3.857784e-04 1.719839e-03 17 45477900 45478100 201 - 1.895 1.706 -0.639
ENSG00000225190 E040 36.0828754 0.0059563466 2.213368e-02 5.595282e-02 17 45478101 45478147 47 - 1.602 1.457 -0.496
ENSG00000225190 E041 35.4628325 0.0089133602 5.630019e-02 1.202415e-01 17 45482437 45482525 89 - 1.579 1.454 -0.429
ENSG00000225190 E042 0.5890081 0.0437384765 3.356385e-01 4.794290e-01 17 45487728 45487862 135 - 0.313 0.146 -1.401
ENSG00000225190 E043 27.8103476 0.0085891275 3.153572e-01 4.581203e-01 17 45490652 45490839 188 - 1.429 1.365 -0.221