ENSG00000224877

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000431388 ENSG00000224877 HEK293_OSMI2_2hA HEK293_TMG_2hB NDUFAF8 protein_coding protein_coding 278.3759 528.0376 137.5522 6.49327 3.809654 -1.940584 241.97667 446.76377 132.2302 36.79320 4.665115 -1.756383 0.8142042 0.8467333 0.9609333 0.1142000 1.353394e-01 1.979943e-10   FALSE
ENST00000576002 ENSG00000224877 HEK293_OSMI2_2hA HEK293_TMG_2hB NDUFAF8 protein_coding protein_coding 278.3759 528.0376 137.5522 6.49327 3.809654 -1.940584 26.11696 67.98082 0.0000 36.61012 0.000000 -12.731124 0.1292917 0.1281667 0.0000000 -0.1281667 1.979943e-10 1.979943e-10   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000224877 E001 3.215812 0.0306078077 5.487466e-01 6.785708e-01 17 81239191 81239267 77 + 0.461 0.565 0.499
ENSG00000224877 E002 3.696281 0.0174405075 9.079384e-01 9.455671e-01 17 81239268 81239304 37 + 0.618 0.585 -0.147
ENSG00000224877 E003 4.388643 0.0047575943 8.712471e-01 9.212053e-01 17 81239305 81239310 6 + 0.680 0.639 -0.172
ENSG00000224877 E004 9.316146 0.0018681400 5.189855e-01 6.531127e-01 17 81239311 81239317 7 + 0.992 0.897 -0.358
ENSG00000224877 E005 411.438884 0.0295228238 8.565174e-06 5.780302e-05 17 81239318 81239403 86 + 2.771 2.410 -1.202
ENSG00000224877 E006 629.063900 0.0153203230 3.004452e-04 1.381348e-03 17 81239404 81239447 44 + 2.852 2.638 -0.712
ENSG00000224877 E007 672.953567 0.0083049416 3.013950e-01 4.431940e-01 17 81239568 81239594 27 + 2.758 2.704 -0.179
ENSG00000224877 E008 689.629598 0.0006140221 2.735115e-03 9.478988e-03 17 81239595 81239610 16 + 2.687 2.731 0.143
ENSG00000224877 E009 805.390604 0.0004215558 8.795522e-03 2.577274e-02 17 81239611 81239628 18 + 2.766 2.795 0.096
ENSG00000224877 E010 1285.830739 0.0004013242 3.177195e-03 1.078885e-02 17 81239629 81239678 50 + 2.973 2.998 0.083
ENSG00000224877 E011 16.912089 0.0012103655 6.374834e-01 7.513707e-01 17 81239679 81239682 4 + 1.113 1.144 0.112
ENSG00000224877 E012 12.198636 0.0016777537 4.415804e-01 5.832252e-01 17 81239683 81239771 89 + 1.091 0.989 -0.376
ENSG00000224877 E013 29.153280 0.0030243157 1.518897e-02 4.091609e-02 17 81239772 81239913 142 + 1.520 1.316 -0.705
ENSG00000224877 E014 18.575952 0.0010325795 2.438827e-02 6.061855e-02 17 81239914 81239943 30 + 1.351 1.134 -0.766
ENSG00000224877 E015 34.833761 0.0005983350 8.491637e-05 4.513316e-04 17 81239944 81240229 286 + 1.654 1.381 -0.939
ENSG00000224877 E016 35.047712 0.0006392630 8.490627e-03 2.501359e-02 17 81240230 81240439 210 + 1.596 1.401 -0.668
ENSG00000224877 E017 3323.308121 0.0100048315 9.176417e-03 2.672392e-02 17 81240987 81241310 324 + 3.354 3.419 0.215