ENSG00000224870

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000418833 ENSG00000224870 HEK293_OSMI2_2hA HEK293_TMG_2hB MRPL20-AS1 lncRNA retained_intron 24.8957 33.40084 15.94238 1.294152 0.5426167 -1.066545 3.523511 2.207977 3.785553 2.207977 0.3347497 0.7750653 0.1537083 0.06143333 0.2390667 0.1776333 0.09741985 0.02328581   FALSE
ENST00000444362 ENSG00000224870 HEK293_OSMI2_2hA HEK293_TMG_2hB MRPL20-AS1 lncRNA lncRNA 24.8957 33.40084 15.94238 1.294152 0.5426167 -1.066545 9.795048 15.149560 4.494510 1.882944 0.2834708 -1.7507858 0.3836750 0.45073333 0.2835000 -0.1672333 0.02328581 0.02328581   FALSE
ENST00000448629 ENSG00000224870 HEK293_OSMI2_2hA HEK293_TMG_2hB MRPL20-AS1 lncRNA lncRNA 24.8957 33.40084 15.94238 1.294152 0.5426167 -1.066545 6.342952 7.651353 4.782594 1.124040 0.6723162 -0.6767923 0.2611792 0.23223333 0.2981333 0.0659000 0.60244082 0.02328581   FALSE
ENST00000659048 ENSG00000224870 HEK293_OSMI2_2hA HEK293_TMG_2hB MRPL20-AS1 lncRNA lncRNA 24.8957 33.40084 15.94238 1.294152 0.5426167 -1.066545 1.151418 3.660442 0.000000 1.835376 0.0000000 -8.5198098 0.0382375 0.11386667 0.0000000 -0.1138667 0.21236237 0.02328581   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000224870 E001 0.4482035 0.0317330999 0.014702281 0.039820194 1 1399515 1399518 4 + 0.395 0.000 -11.588
ENSG00000224870 E002 0.4482035 0.0317330999 0.014702281 0.039820194 1 1399519 1399521 3 + 0.395 0.000 -13.490
ENSG00000224870 E003 1.8454999 0.0272825139 0.396100748 0.539884012 1 1399522 1399529 8 + 0.540 0.396 -0.731
ENSG00000224870 E004 11.2745257 0.0015680703 0.785878582 0.862865934 1 1399530 1399540 11 + 1.017 1.052 0.131
ENSG00000224870 E005 14.6304143 0.0012115714 0.687650993 0.789947595 1 1399541 1399546 6 + 1.109 1.155 0.163
ENSG00000224870 E006 16.1082496 0.0011589297 0.446056263 0.587592451 1 1399547 1399549 3 + 1.126 1.204 0.279
ENSG00000224870 E007 18.9290676 0.0010248602 0.472928645 0.611956181 1 1399550 1399551 2 + 1.199 1.269 0.244
ENSG00000224870 E008 26.8812281 0.0007659644 0.337211969 0.481081660 1 1399552 1399561 10 + 1.338 1.416 0.272
ENSG00000224870 E009 42.6314206 0.0005395907 0.958606843 0.978062283 1 1399562 1399571 10 + 1.586 1.590 0.013
ENSG00000224870 E010 112.9592469 0.0003366831 0.082159552 0.162928450 1 1399572 1399685 114 + 2.040 1.985 -0.186
ENSG00000224870 E011 39.7102538 0.0007417716 0.033447018 0.078674412 1 1399686 1399689 4 + 1.643 1.526 -0.401
ENSG00000224870 E012 44.1472957 0.0004856593 0.951612278 0.973701504 1 1399690 1399805 116 + 1.592 1.602 0.035
ENSG00000224870 E013 29.0207069 0.0007339103 0.811512760 0.880378843 1 1399806 1399834 29 + 1.427 1.416 -0.035
ENSG00000224870 E014 66.8410308 0.0003887989 0.397249745 0.541021922 1 1399835 1399980 146 + 1.798 1.765 -0.110
ENSG00000224870 E015 82.0096337 0.0003330315 0.062950514 0.131632646 1 1399981 1400157 177 + 1.914 1.844 -0.237
ENSG00000224870 E016 159.5085327 0.0003738125 0.090977039 0.176678969 1 1400158 1400249 92 + 2.181 2.136 -0.149
ENSG00000224870 E017 120.1262592 0.0031432624 0.403068572 0.546860948 1 1400250 1400303 54 + 2.050 2.020 -0.100
ENSG00000224870 E018 23.9842931 0.0104778051 0.269767784 0.408324391 1 1400304 1400418 115 + 1.410 1.317 -0.324
ENSG00000224870 E019 17.7627589 0.0010185419 0.452717635 0.593548544 1 1400419 1400472 54 + 1.262 1.204 -0.206
ENSG00000224870 E020 19.8265585 0.0009229376 0.131965697 0.236848140 1 1400473 1400604 132 + 1.347 1.232 -0.403
ENSG00000224870 E021 600.0364142 0.0001207136 0.948667263 0.971823130 1 1400605 1401242 638 + 2.718 2.725 0.020
ENSG00000224870 E022 576.4839063 0.0014319086 0.000395772 0.001758227 1 1401243 1402046 804 + 2.647 2.726 0.262