ENSG00000224616

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000421185 ENSG00000224616 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.747811 2.837885 1.329299 0.3843685 0.3232806 -1.088413 0.44323887 1.2843067 0.2412314 0.83236089 0.12311106 -2.3650909 0.1747375 0.39260000 0.18396667 -0.20863333 0.859828473 0.005717743   FALSE
ENST00000653267 ENSG00000224616 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.747811 2.837885 1.329299 0.3843685 0.3232806 -1.088413 0.45512464 0.1975542 0.3670876 0.09899998 0.07564095 0.8614117 0.3760167 0.07770000 0.28650000 0.20880000 0.211355346 0.005717743 FALSE TRUE
ENST00000666916 ENSG00000224616 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.747811 2.837885 1.329299 0.3843685 0.3232806 -1.088413 0.04694681 0.0000000 0.1125157 0.00000000 0.05664310 3.6148949 0.0363250 0.00000000 0.08413333 0.08413333 0.058102008 0.005717743   FALSE
ENST00000670421 ENSG00000224616 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA lncRNA 1.747811 2.837885 1.329299 0.3843685 0.3232806 -1.088413 0.34037281 0.2271296 0.4090329 0.01244226 0.01631518 0.8213881 0.2749292 0.08493333 0.34446667 0.25953333 0.005717743 0.005717743 FALSE TRUE
MSTRG.1692.6 ENSG00000224616 HEK293_OSMI2_2hA HEK293_TMG_2hB   lncRNA   1.747811 2.837885 1.329299 0.3843685 0.3232806 -1.088413 0.44360530 1.1288946 0.1875780 0.57292611 0.18757803 -2.5271398 0.1217792 0.44480000 0.09496667 -0.34983333 0.518409921 0.005717743   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000224616 E001 0.3030308 0.242859293 2.790853e-01   1 100251528 100253821 2294 - 0.221 0.000 -10.513
ENSG00000224616 E002 0.1515154 0.042397982 5.149758e-01   1 100258354 100258363 10 - 0.124 0.000 -9.989
ENSG00000224616 E003 21.9572031 0.043119788 2.089351e-02 5.336459e-02 1 100258364 100261226 2863 - 1.489 1.205 -0.989
ENSG00000224616 E004 5.5851208 0.003310994 6.408039e-07 5.547105e-06 1 100261227 100261862 636 - 1.076 0.358 -3.092
ENSG00000224616 E005 4.8059973 0.031954477 1.068123e-02 3.040887e-02 1 100261863 100262804 942 - 0.935 0.513 -1.753
ENSG00000224616 E006 7.0408801 0.072357668 1.886795e-02 4.904405e-02 1 100262805 100263992 1188 - 1.076 0.662 -1.601
ENSG00000224616 E007 1.1198269 0.020665706 7.651481e-01 8.479828e-01 1 100263993 100264008 16 - 0.366 0.292 -0.465
ENSG00000224616 E008 1.9335024 0.009559208 6.301674e-01 7.453818e-01 1 100264009 100264031 23 - 0.521 0.415 -0.534
ENSG00000224616 E009 13.7112005 0.001516763 9.531240e-03 2.761504e-02 1 100264032 100265067 1036 - 1.283 1.026 -0.921
ENSG00000224616 E010 4.1445772 0.004181475 2.955278e-01 4.366638e-01 1 100265068 100265152 85 - 0.799 0.623 -0.726
ENSG00000224616 E011 4.5959012 0.004059470 2.445093e-01 3.794796e-01 1 100265153 100265247 95 - 0.842 0.655 -0.759
ENSG00000224616 E012 9.0384639 0.002410236 4.858859e-03 1.553921e-02 1 100265248 100265814 567 - 0.842 1.108 0.990
ENSG00000224616 E013 7.2860045 0.041662675 2.197184e-01 3.500487e-01 1 100265815 100265828 14 - 0.843 0.990 0.555
ENSG00000224616 E014 19.2519089 0.003908958 4.250261e-08 4.651104e-07 1 100265829 100265917 89 - 1.051 1.449 1.402
ENSG00000224616 E015 18.8329388 0.001203144 1.311420e-08 1.582046e-07 1 100266004 100266179 176 - 1.038 1.440 1.419