ENSG00000224281

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000395539 ENSG00000224281 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC25A5-AS1 lncRNA retained_intron 0.8015884 1.150769 0.647542 0.02838683 0.07767357 -0.8199265 0.55477715 0.83051469 0.20582344 0.03381358 0.02194812 -1.9614215 0.68218333 0.72353333 0.33366667 -0.38986667 0.005494015 0.005494015 FALSE FALSE
ENST00000445759 ENSG00000224281 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC25A5-AS1 lncRNA lncRNA 0.8015884 1.150769 0.647542 0.02838683 0.07767357 -0.8199265 0.03576641 0.01958672 0.05028281 0.01958672 0.05028281 1.0267970 0.06631667 0.01766667 0.09023333 0.07256667 0.894309417 0.005494015   FALSE
ENST00000446986 ENSG00000224281 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC25A5-AS1 lncRNA lncRNA 0.8015884 1.150769 0.647542 0.02838683 0.07767357 -0.8199265 0.06974374 0.18223585 0.12918816 0.01566292 0.06509187 -0.4658409 0.10214167 0.15816667 0.22630000 0.06813333 0.963437404 0.005494015 TRUE FALSE
ENST00000609227 ENSG00000224281 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC25A5-AS1 lncRNA lncRNA 0.8015884 1.150769 0.647542 0.02838683 0.07767357 -0.8199265 0.08362129 0.08125498 0.00000000 0.08125498 0.00000000 -3.1899033 0.08159583 0.06743333 0.00000000 -0.06743333 0.848630306 0.005494015   FALSE
ENST00000666209 ENSG00000224281 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC25A5-AS1 lncRNA lncRNA 0.8015884 1.150769 0.647542 0.02838683 0.07767357 -0.8199265 0.03558515 0.02243370 0.26224754 0.02243370 0.19365966 3.0693536 0.04625000 0.02023333 0.34976667 0.32953333 0.352793485 0.005494015   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000224281 E001 0.000000       X 119465986 119466033 48 -      
ENSG00000224281 E002 0.699736 0.016846356 0.266025685 0.404152108 X 119466034 119466067 34 - 0.001 0.243 9.134
ENSG00000224281 E003 2.957849 0.007251748 0.752961252 0.839056105 X 119466068 119466363 296 - 0.513 0.559 0.213
ENSG00000224281 E004 1.367107 0.013000896 0.591922541 0.714583813 X 119466364 119466423 60 - 0.431 0.326 -0.597
ENSG00000224281 E005 21.043336 0.040447613 0.029875643 0.071719943 X 119466424 119467306 883 - 1.431 1.208 -0.779
ENSG00000224281 E006 10.543673 0.001732386 0.011519981 0.032395468 X 119467307 119467750 444 - 1.180 0.919 -0.951
ENSG00000224281 E007 9.181564 0.002322615 0.002778986 0.009610811 X 119467751 119468105 355 - 0.583 0.992 1.641
ENSG00000224281 E008 27.287526 0.010864776 0.073646626 0.149369236 X 119468106 119468809 704 - 1.268 1.390 0.426
ENSG00000224281 E009 10.584728 0.063541628 0.474604651 0.613418481 X 119468810 119468981 172 - 0.921 1.006 0.316
ENSG00000224281 E010 5.515403 0.016739036 0.587919515 0.711167559 X 119468982 119469128 147 - 0.695 0.767 0.295