ENSG00000223501

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000445902 ENSG00000223501 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS52 protein_coding protein_coding 35.16143 40.6358 32.3204 1.016844 0.5379143 -0.3302149 19.9326470 26.8759067 17.804284 1.5111380 0.8749926 -0.5938156 0.56421250 0.66040000 0.55113333 -0.10926667 9.530291e-02 3.440284e-23 FALSE  
ENST00000461743 ENSG00000223501 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS52 protein_coding retained_intron 35.16143 40.6358 32.3204 1.016844 0.5379143 -0.3302149 1.7561951 0.7908508 1.545768 0.2081192 0.1198325 0.9580220 0.05125833 0.01926667 0.04780000 0.02853333 9.610633e-03 3.440284e-23    
ENST00000463486 ENSG00000223501 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS52 protein_coding processed_transcript 35.16143 40.6358 32.3204 1.016844 0.5379143 -0.3302149 0.5314254 0.0000000 1.778630 0.0000000 0.1956739 7.4827113 0.01694167 0.00000000 0.05503333 0.05503333 3.440284e-23 3.440284e-23 FALSE  
ENST00000493379 ENSG00000223501 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS52 protein_coding retained_intron 35.16143 40.6358 32.3204 1.016844 0.5379143 -0.3302149 2.9024804 1.4001161 2.792038 0.5503579 0.3937694 0.9906624 0.08441667 0.03496667 0.08610000 0.05113333 1.010196e-01 3.440284e-23 TRUE  
ENST00000495981 ENSG00000223501 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS52 protein_coding processed_transcript 35.16143 40.6358 32.3204 1.016844 0.5379143 -0.3302149 1.9838319 1.0707299 1.361349 0.2496931 0.3491830 0.3435899 0.05667917 0.02646667 0.04176667 0.01530000 5.184740e-01 3.440284e-23    
MSTRG.28118.1 ENSG00000223501 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS52 protein_coding   35.16143 40.6358 32.3204 1.016844 0.5379143 -0.3302149 3.0564074 5.8268422 2.396536 0.6460259 0.4775120 -1.2782300 0.08414583 0.14363333 0.07440000 -0.06923333 6.415921e-02 3.440284e-23 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000223501 E001 6.029843 0.0346207170 1.688155e-01 2.866401e-01 6 33250231 33250271 41 - 0.956 0.757 -0.771
ENSG00000223501 E002 5.218445 0.0594310464 2.698551e-01 4.084076e-01 6 33250272 33250277 6 - 0.891 0.699 -0.761
ENSG00000223501 E003 440.813808 0.0047145829 8.486455e-08 8.736257e-07 6 33250278 33250605 328 - 2.478 2.674 0.655
ENSG00000223501 E004 444.056270 0.0016817044 1.387235e-07 1.371862e-06 6 33250606 33250810 205 - 2.527 2.663 0.451
ENSG00000223501 E005 285.734694 0.0010418091 1.371463e-06 1.105662e-05 6 33250811 33250919 109 - 2.342 2.471 0.429
ENSG00000223501 E006 200.772636 0.0003077074 6.300177e-08 6.653750e-07 6 33250920 33250987 68 - 2.174 2.323 0.498
ENSG00000223501 E007 194.583417 0.0021311043 5.876606e-03 1.828808e-02 6 33251518 33251636 119 - 2.199 2.291 0.309
ENSG00000223501 E008 189.664025 0.0004988737 6.913171e-01 7.928178e-01 6 33251860 33251916 57 - 2.246 2.255 0.030
ENSG00000223501 E009 165.151078 0.0003508904 6.956940e-02 1.426473e-01 6 33251917 33251971 55 - 2.157 2.209 0.174
ENSG00000223501 E010 224.579759 0.0002255039 3.105484e-02 7.402080e-02 6 33263484 33263547 64 - 2.293 2.346 0.177
ENSG00000223501 E011 157.168572 0.0002586738 8.450216e-02 1.665992e-01 6 33263548 33263549 2 - 2.141 2.191 0.168
ENSG00000223501 E012 16.275534 0.0015977827 4.822140e-04 2.092108e-03 6 33263550 33263771 222 - 1.385 1.089 -1.043
ENSG00000223501 E013 161.333644 0.0009382097 1.266058e-01 2.294192e-01 6 33263772 33263782 11 - 2.154 2.202 0.159
ENSG00000223501 E014 244.377853 0.0002240287 3.502284e-03 1.173209e-02 6 33263783 33263879 97 - 2.317 2.387 0.232
ENSG00000223501 E015 242.530119 0.0001861192 1.883440e-03 6.863090e-03 6 33264008 33264078 71 - 2.311 2.385 0.247
ENSG00000223501 E016 187.582807 0.0002329408 1.312981e-02 3.621025e-02 6 33264079 33264103 25 - 2.206 2.273 0.224
ENSG00000223501 E017 13.184558 0.0013797773 5.363423e-05 2.999157e-04 6 33264104 33264303 200 - 1.337 0.967 -1.324
ENSG00000223501 E018 271.438059 0.0002276183 3.247808e-03 1.099516e-02 6 33264374 33264497 124 - 2.365 2.432 0.223
ENSG00000223501 E019 38.078937 0.0158858723 1.889957e-04 9.168928e-04 6 33264498 33264781 284 - 1.745 1.435 -1.057
ENSG00000223501 E020 253.836069 0.0003292354 3.956717e-01 5.394735e-01 6 33264782 33264900 119 - 2.368 2.387 0.063
ENSG00000223501 E021 168.498591 0.0002177977 1.627549e-01 2.787380e-01 6 33266557 33266609 53 - 2.179 2.218 0.129
ENSG00000223501 E022 200.002993 0.0002634640 5.189541e-01 6.530906e-01 6 33266610 33266712 103 - 2.268 2.283 0.051
ENSG00000223501 E023 193.991062 0.0001952714 1.842384e-01 3.065495e-01 6 33267188 33267321 134 - 2.242 2.276 0.113
ENSG00000223501 E024 2.584024 0.0068643823 6.276313e-03 1.933663e-02 6 33267466 33267681 216 - 0.783 0.353 -2.018
ENSG00000223501 E025 164.463073 0.0002329741 8.816141e-01 9.281265e-01 6 33267682 33267739 58 - 2.193 2.196 0.009
ENSG00000223501 E026 126.909867 0.0002639874 7.548318e-02 1.522855e-01 6 33267865 33267869 5 - 2.122 2.063 -0.199
ENSG00000223501 E027 126.345851 0.0002609799 8.106382e-02 1.612112e-01 6 33267870 33267870 1 - 2.119 2.061 -0.196
ENSG00000223501 E028 251.548001 0.0001697467 2.965280e-02 7.128435e-02 6 33267871 33267997 127 - 2.411 2.359 -0.174
ENSG00000223501 E029 200.348643 0.0003614103 3.835167e-04 1.710843e-03 6 33268108 33268208 101 - 2.341 2.246 -0.320
ENSG00000223501 E030 231.660495 0.0009237668 9.615743e-04 3.826965e-03 6 33268499 33268649 151 - 2.401 2.310 -0.305
ENSG00000223501 E031 259.412121 0.0039752323 3.266706e-06 2.425030e-05 6 33269014 33269189 176 - 2.498 2.322 -0.585
ENSG00000223501 E032 2.985110 0.0052158634 2.490051e-02 6.166839e-02 6 33269190 33269218 29 - 0.783 0.440 -1.532
ENSG00000223501 E033 142.876723 0.0074651934 1.052852e-04 5.464348e-04 6 33269490 33269557 68 - 2.254 2.051 -0.677
ENSG00000223501 E034 98.193566 0.0044636214 2.653821e-11 5.156497e-10 6 33269744 33269812 69 - 2.155 1.834 -1.080
ENSG00000223501 E035 47.571817 0.0008867710 2.138663e-22 1.781721e-20 6 33269813 33269819 7 - 1.930 1.424 -1.719
ENSG00000223501 E036 73.316325 0.0004554874 4.424247e-23 3.959189e-21 6 33269999 33270051 53 - 2.074 1.661 -1.389
ENSG00000223501 E037 125.499825 0.0064628433 1.007826e-07 1.022281e-06 6 33270199 33270283 85 - 2.234 1.966 -0.898
ENSG00000223501 E038 11.362918 0.0016544291 6.272618e-05 3.448129e-04 6 33271137 33271585 449 - 1.283 0.890 -1.425
ENSG00000223501 E039 125.055472 0.0097122202 9.984437e-03 2.872514e-02 6 33271586 33271690 105 - 2.169 2.016 -0.510
ENSG00000223501 E040 127.563074 0.0133307822 9.599257e-02 1.843605e-01 6 33271691 33271884 194 - 2.149 2.043 -0.353
ENSG00000223501 E041 2.360498 0.0064013047 6.768220e-01 7.816788e-01 6 33271905 33272047 143 - 0.549 0.478 -0.339