ENSG00000221914

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000315985 ENSG00000221914 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP2R2A protein_coding protein_coding 35.53441 21.22324 53.49342 0.523242 3.159624 1.333307 3.067892 10.0743428 0.00000 1.7866298 0.0000000 -9.977901 0.1467917 0.4715667 0.0000000 -0.47156667 6.034313e-30 6.034313e-30 FALSE TRUE
ENST00000380737 ENSG00000221914 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP2R2A protein_coding protein_coding 35.53441 21.22324 53.49342 0.523242 3.159624 1.333307 16.177905 8.6222089 25.00804 0.8509023 0.9948713 1.535167 0.4642208 0.4084333 0.4686667 0.06023333 6.198739e-01 6.034313e-30 FALSE TRUE
ENST00000523925 ENSG00000221914 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP2R2A protein_coding protein_coding 35.53441 21.22324 53.49342 0.523242 3.159624 1.333307 11.581340 0.6181141 21.94256 0.6181141 1.9557323 5.127219 0.2476833 0.0296000 0.4086667 0.37906667 1.899215e-02 6.034313e-30   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000221914 E001 1.6596189 0.0171809926 1.415739e-01 2.501842e-01 8 26291508 26291519 12 + 0.501 0.274 -1.308
ENSG00000221914 E002 4.1414436 0.0038333264 6.361092e-01 7.502131e-01 8 26291520 26291527 8 + 0.708 0.695 -0.054
ENSG00000221914 E003 4.1414436 0.0038333264 6.361092e-01 7.502131e-01 8 26291528 26291529 2 + 0.708 0.695 -0.054
ENSG00000221914 E004 5.8271875 0.0028108707 1.266443e-01 2.294670e-01 8 26291530 26291538 9 + 0.876 0.732 -0.569
ENSG00000221914 E005 15.4231035 0.0144228161 9.304099e-01 9.602179e-01 8 26291539 26291613 75 + 1.176 1.229 0.188
ENSG00000221914 E006 15.2520082 0.0155471115 8.502947e-01 9.071371e-01 8 26291614 26291653 40 + 1.176 1.218 0.147
ENSG00000221914 E007 12.2478950 0.0196934100 7.906525e-01 8.660997e-01 8 26291654 26291687 34 + 1.065 1.156 0.326
ENSG00000221914 E008 106.5277300 0.0003995992 2.339469e-11 4.589474e-10 8 26291688 26291826 139 + 2.084 1.906 -0.599
ENSG00000221914 E009 0.1451727 0.0433771120 7.334779e-01   8 26291835 26291981 147 + 0.094 0.000 -12.558
ENSG00000221914 E010 1.0351233 0.0121064492 1.677491e-02 4.444010e-02 8 26291982 26292007 26 + 0.430 0.000 -15.365
ENSG00000221914 E011 0.0000000       8 26292113 26292116 4 +      
ENSG00000221914 E012 0.0000000       8 26292117 26292118 2 +      
ENSG00000221914 E013 0.8868276 0.0253694555 9.653459e-01 9.822010e-01 8 26292119 26292284 166 + 0.236 0.274 0.284
ENSG00000221914 E014 1.2147488 0.0115650157 3.415018e-01 4.853960e-01 8 26292285 26292441 157 + 0.236 0.441 1.280
ENSG00000221914 E015 0.2944980 0.4622290146 4.794834e-01   8 26293112 26293154 43 + 0.171 0.000 -13.557
ENSG00000221914 E016 0.1515154 0.0427540171 7.333743e-01   8 26293206 26293215 10 + 0.094 0.000 -12.559
ENSG00000221914 E017 0.1515154 0.0427540171 7.333743e-01   8 26293216 26293216 1 + 0.094 0.000 -12.559
ENSG00000221914 E018 0.6256415 0.0172671820 5.591460e-01 6.872853e-01 8 26293217 26293283 67 + 0.236 0.158 -0.721
ENSG00000221914 E019 0.8470867 0.0136968632 9.673176e-01 9.835362e-01 8 26293284 26293366 83 + 0.236 0.274 0.280
ENSG00000221914 E020 0.6998377 0.0167317263 6.611177e-01 7.696458e-01 8 26293483 26293571 89 + 0.171 0.274 0.865
ENSG00000221914 E021 0.3729606 0.0317284140 7.451794e-01 8.333108e-01 8 26293572 26293589 18 + 0.094 0.158 0.866
ENSG00000221914 E022 0.9536514 0.2308057874 3.715292e-01 5.159820e-01 8 26293590 26293665 76 + 0.343 0.160 -1.432
ENSG00000221914 E023 86.2274826 0.0015890456 4.863997e-09 6.361544e-08 8 26293666 26293668 3 + 1.997 1.805 -0.648
ENSG00000221914 E024 172.3991566 0.0029840393 2.938463e-06 2.203202e-05 8 26293669 26293740 72 + 2.270 2.159 -0.371
ENSG00000221914 E025 0.6643356 0.8060519414 2.168725e-01 3.466370e-01 8 26293741 26293860 120 + 0.000 0.390 15.741
ENSG00000221914 E026 0.0000000       8 26298575 26298608 34 +      
ENSG00000221914 E027 0.1451727 0.0433771120 7.334779e-01   8 26304534 26304621 88 + 0.094 0.000 -12.558
ENSG00000221914 E028 0.2214452 0.0412316966 3.176446e-01   8 26325293 26325427 135 + 0.000 0.158 14.014
ENSG00000221914 E029 1.6523458 0.0089875661 2.580179e-02 6.353261e-02 8 26333486 26333570 85 + 0.533 0.158 -2.458
ENSG00000221914 E030 109.6957721 0.0043079747 1.895019e-03 6.898781e-03 8 26338890 26338892 3 + 2.064 1.986 -0.263
ENSG00000221914 E031 223.9907782 0.0001762844 2.934060e-13 7.805769e-12 8 26338893 26338987 95 + 2.384 2.272 -0.375
ENSG00000221914 E032 7.7409504 0.0046503828 1.503408e-04 7.485115e-04 8 26339903 26339973 71 + 1.056 0.610 -1.754
ENSG00000221914 E033 1.1950578 0.2128552003 3.107051e-02 7.405069e-02 8 26350599 26350681 83 + 0.468 0.000 -15.563
ENSG00000221914 E034 2.0306170 0.0081972581 2.264007e-01 3.580959e-01 8 26353811 26354418 608 + 0.533 0.365 -0.873
ENSG00000221914 E035 1.0801878 0.0450547905 1.697634e-01 2.878988e-01 8 26354419 26354467 49 + 0.389 0.158 -1.722
ENSG00000221914 E036 317.3285293 0.0003853095 2.333467e-08 2.684897e-07 8 26354468 26354633 166 + 2.515 2.461 -0.178
ENSG00000221914 E037 0.0000000       8 26354634 26354637 4 +      
ENSG00000221914 E038 0.2987644 0.0274044400 3.509779e-01   8 26358907 26358911 5 + 0.171 0.000 -13.558
ENSG00000221914 E039 1.0747755 0.0385603495 5.706442e-01 6.970677e-01 8 26358912 26359032 121 + 0.344 0.273 -0.462
ENSG00000221914 E040 270.7840623 0.0013286196 3.757348e-02 8.653542e-02 8 26360169 26360281 113 + 2.421 2.431 0.034
ENSG00000221914 E041 0.4375944 0.0280962976 1.786130e-01 2.994168e-01 8 26360457 26360555 99 + 0.236 0.000 -14.143
ENSG00000221914 E042 1.1887151 0.0183081557 1.016696e-02 2.917625e-02 8 26360600 26360973 374 + 0.467 0.000 -15.558
ENSG00000221914 E043 303.2814301 0.0001964124 1.309193e-01 2.354272e-01 8 26360974 26361089 116 + 2.458 2.495 0.121
ENSG00000221914 E044 152.9154037 0.0002406619 3.099348e-02 7.390523e-02 8 26361090 26361100 11 + 2.177 2.182 0.016
ENSG00000221914 E045 248.6224965 0.0002026850 9.488489e-01 9.719408e-01 8 26361101 26361151 51 + 2.358 2.428 0.232
ENSG00000221914 E046 440.0494057 0.0006967182 9.771694e-01 9.897657e-01 8 26362684 26362848 165 + 2.607 2.673 0.222
ENSG00000221914 E047 30.5277532 0.0006610199 3.795662e-09 5.053601e-08 8 26362849 26363720 872 + 1.600 1.258 -1.183
ENSG00000221914 E048 452.7351316 0.0024705380 8.444274e-01 9.031714e-01 8 26363721 26363890 170 + 2.618 2.689 0.239
ENSG00000221914 E049 12.9476537 0.0081622131 3.672942e-08 4.064178e-07 8 26365098 26366314 1217 + 1.292 0.694 -2.237
ENSG00000221914 E050 326.7802254 0.0040668873 3.199954e-01 4.630898e-01 8 26366315 26366406 92 + 2.463 2.564 0.335
ENSG00000221914 E051 1439.6905206 0.0084417131 1.373259e-05 8.834551e-05 8 26370134 26372680 2547 + 3.051 3.266 0.712