ENSG00000218891

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000325421 ENSG00000218891 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF579 protein_coding protein_coding 14.3822 20.68401 9.987868 1.881815 0.6975684 -1.049521 1.816637 3.3670895 0.8269053 0.3443812 0.02756065 -2.0126441 0.1217708 0.16893333 0.0841000 -0.08483333 0.0990135823 0.0001343331 FALSE TRUE
ENST00000592239 ENSG00000218891 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF579 protein_coding protein_coding 14.3822 20.68401 9.987868 1.881815 0.6975684 -1.049521 1.815163 0.9826394 1.7280596 0.2245961 0.34699889 0.8081359 0.1685292 0.04713333 0.1696000 0.12246667 0.0001343331 0.0001343331   FALSE
MSTRG.17721.4 ENSG00000218891 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF579 protein_coding   14.3822 20.68401 9.987868 1.881815 0.6975684 -1.049521 10.508574 15.9593408 7.0282157 1.9601688 0.24778479 -1.1820231 0.6934333 0.76706667 0.7076667 -0.05940000 0.5565695704 0.0001343331 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000218891 E001 0.5149111 0.020530290 7.509787e-01 8.375230e-01 19 55576774 55576860 87 - 0.194 0.138 -0.590
ENSG00000218891 E002 2.7249380 0.006140771 6.085938e-01 7.282926e-01 19 55576861 55576950 90 - 0.582 0.486 -0.453
ENSG00000218891 E003 8.7701740 0.002029455 5.694366e-01 6.959875e-01 19 55576951 55577377 427 - 0.858 0.920 0.236
ENSG00000218891 E004 2.2434236 0.039881194 4.824442e-01 6.205788e-01 19 55577378 55577411 34 - 0.328 0.462 0.746
ENSG00000218891 E005 1.9478814 0.077485152 2.524435e-01 3.887974e-01 19 55577412 55577436 25 - 0.194 0.435 1.611
ENSG00000218891 E006 42.3714099 0.022911813 2.171718e-02 5.508810e-02 19 55577437 55577579 143 - 1.373 1.597 0.769
ENSG00000218891 E007 152.9268372 0.004155106 7.484748e-03 2.247275e-02 19 55577580 55577730 151 - 2.019 2.124 0.354
ENSG00000218891 E008 292.0029434 0.005136095 1.499027e-08 1.788777e-07 19 55577731 55577998 268 - 2.217 2.418 0.672
ENSG00000218891 E009 645.8626244 0.005765322 1.216792e-03 4.696964e-03 19 55577999 55579593 1595 - 2.782 2.708 -0.247
ENSG00000218891 E010 47.0292314 0.008010565 5.616039e-04 2.390612e-03 19 55579594 55579641 48 - 1.768 1.527 -0.819
ENSG00000218891 E011 15.3436712 0.006104338 4.086438e-13 1.065900e-11 19 55579642 55580039 398 - 1.543 0.869 -2.404
ENSG00000218891 E012 37.8714191 0.008911833 3.818500e-03 1.263256e-02 19 55580795 55580880 86 - 1.664 1.443 -0.759