ENSG00000215717

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000338272 ENSG00000215717 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM167B protein_coding protein_coding 11.03348 8.319997 15.44849 0.9593441 0.383777 0.8920115 8.8389410 5.4202730 13.6343787 0.4743867 0.2445177 1.3292101 0.7489625 0.6570667 0.88363333 0.22656667 2.201706e-03 8.185096e-10 FALSE FALSE
MSTRG.1735.4 ENSG00000215717 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM167B protein_coding   11.03348 8.319997 15.44849 0.9593441 0.383777 0.8920115 0.9970437 2.2007231 0.3400179 0.5008215 0.1005048 -2.6590179 0.1383250 0.2590667 0.02176667 -0.23730000 8.185096e-10 8.185096e-10 FALSE FALSE
MSTRG.1735.5 ENSG00000215717 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM167B protein_coding   11.03348 8.319997 15.44849 0.9593441 0.383777 0.8920115 0.5002614 0.4883876 0.6786455 0.1200595 0.3712181 0.4664935 0.0519875 0.0574000 0.04306667 -0.01433333 7.588342e-01 8.185096e-10   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000215717 E001 2.9893620 0.0054215222 4.544922e-01 5.951338e-01 1 109090764 109090772 9 + 0.546 0.641 0.426
ENSG00000215717 E002 11.1475907 0.0023261981 7.227935e-01 8.168307e-01 1 109090773 109090779 7 + 1.107 1.046 -0.224
ENSG00000215717 E003 19.8930154 0.0009433869 9.215207e-01 9.544601e-01 1 109090780 109090786 7 + 1.334 1.300 -0.120
ENSG00000215717 E004 38.6390010 0.0006245229 2.487078e-02 6.160688e-02 1 109090787 109090805 19 + 1.558 1.655 0.330
ENSG00000215717 E005 80.0278699 0.0003902474 3.501680e-06 2.582965e-05 1 109090806 109090882 77 + 1.839 1.989 0.503
ENSG00000215717 E006 0.9609246 0.0130799526 8.987511e-01 9.394229e-01 1 109091660 109091748 89 + 0.302 0.266 -0.253
ENSG00000215717 E007 115.5227518 0.0014916843 1.696528e-05 1.069414e-04 1 109092890 109093021 132 + 2.011 2.131 0.402
ENSG00000215717 E008 3.7083231 0.0043348766 1.303379e-04 6.602702e-04 1 109093022 109093082 61 + 0.847 0.153 -3.839
ENSG00000215717 E009 8.0648823 0.0020759053 2.297637e-02 5.769966e-02 1 109093083 109093776 694 + 1.053 0.751 -1.151
ENSG00000215717 E010 632.0167742 0.0017208171 3.238294e-09 4.366753e-08 1 109094417 109096769 2353 + 2.836 2.738 -0.328
ENSG00000215717 E011 40.4763276 0.0007423536 2.963439e-04 1.365142e-03 1 109096770 109096955 186 + 1.543 1.712 0.575