ENSG00000214517

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000328257 ENSG00000214517 HEK293_OSMI2_2hA HEK293_TMG_2hB PPME1 protein_coding protein_coding 55.94081 71.93113 47.39339 2.279791 0.8251487 -0.6018267 49.208595 65.137228 39.401820 2.4836694 0.9410874 -0.7250754 0.87580417 0.90546667 0.8312000 -0.07426667 2.473802e-02 6.000991e-08 FALSE  
ENST00000542710 ENSG00000214517 HEK293_OSMI2_2hA HEK293_TMG_2hB PPME1 protein_coding processed_transcript 55.94081 71.93113 47.39339 2.279791 0.8251487 -0.6018267 5.128355 4.789718 6.547752 0.2377165 0.1956841 0.4502519 0.09557917 0.06673333 0.1383333 0.07160000 6.000991e-08 6.000991e-08 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000214517 E001 31.1509794 0.0006474250 2.515673e-02 6.220135e-02 11 74171267 74171288 22 + 1.549 1.440 -0.375
ENSG00000214517 E002 104.9882433 0.0003205677 2.905442e-07 2.696454e-06 11 74171289 74171321 33 + 2.091 1.944 -0.495
ENSG00000214517 E003 154.2861147 0.0002401518 1.550805e-09 2.220004e-08 11 74171322 74171342 21 + 2.255 2.112 -0.477
ENSG00000214517 E004 378.2970417 0.0024567082 3.052530e-07 2.819564e-06 11 74171343 74171522 180 + 2.628 2.512 -0.389
ENSG00000214517 E005 344.7487115 0.0001832289 3.493145e-14 1.060692e-12 11 74203728 74203821 94 + 2.588 2.472 -0.387
ENSG00000214517 E006 336.5174545 0.0009185345 3.348740e-05 1.968136e-04 11 74204353 74204445 93 + 2.546 2.479 -0.223
ENSG00000214517 E007 0.7770552 0.0149758242 8.607157e-01 9.141519e-01 11 74205777 74205923 147 + 0.262 0.247 -0.118
ENSG00000214517 E008 288.5578663 0.0011954721 2.098085e-03 7.528379e-03 11 74222312 74222369 58 + 2.469 2.420 -0.165
ENSG00000214517 E009 0.1472490 0.0435469186 2.843834e-01   11 74222671 74222733 63 + 0.151 0.000 -9.932
ENSG00000214517 E010 281.1336204 0.0002150767 9.057304e-02 1.760309e-01 11 74225205 74225256 52 + 2.430 2.426 -0.013
ENSG00000214517 E011 470.8211086 0.0001148647 7.912838e-02 1.581207e-01 11 74230245 74230399 155 + 2.648 2.652 0.014
ENSG00000214517 E012 1.3651183 0.0099691492 9.435033e-01 9.685535e-01 11 74230785 74230911 127 + 0.351 0.357 0.037
ENSG00000214517 E013 209.2364326 0.0001891112 1.683523e-01 2.860478e-01 11 74230912 74230917 6 + 2.300 2.299 -0.005
ENSG00000214517 E014 390.4527157 0.0001562604 1.780241e-03 6.536832e-03 11 74230918 74231002 85 + 2.586 2.561 -0.083
ENSG00000214517 E015 1.1718769 0.0400631898 2.119321e-04 1.014988e-03 11 74235725 74235900 176 + 0.635 0.000 -12.930
ENSG00000214517 E016 400.3868167 0.0001344898 1.007570e-01 1.916131e-01 11 74235901 74235966 66 + 2.578 2.581 0.011
ENSG00000214517 E017 10.9348026 0.0016010642 1.590686e-05 1.009347e-04 11 74237788 74238892 1105 + 1.275 0.883 -1.427
ENSG00000214517 E018 4.8831261 0.0034612647 4.717230e-04 2.052002e-03 11 74238893 74239132 240 + 0.987 0.551 -1.771
ENSG00000214517 E019 565.8906935 0.0015151433 8.171298e-01 8.843448e-01 11 74239133 74239256 124 + 2.704 2.742 0.126
ENSG00000214517 E020 3.3224800 0.0048363793 4.296335e-01 5.723340e-01 11 74246034 74246075 42 + 0.675 0.581 -0.411
ENSG00000214517 E021 495.1666380 0.0011708766 2.362286e-02 5.903441e-02 11 74246076 74246205 130 + 2.614 2.700 0.285
ENSG00000214517 E022 273.1496583 0.0018274776 1.148468e-02 3.231744e-02 11 74247079 74247123 45 + 2.337 2.448 0.369
ENSG00000214517 E023 8.2923795 0.0144560942 1.457525e-05 9.323777e-05 11 74248243 74250953 2711 + 1.214 0.725 -1.832
ENSG00000214517 E024 325.0208499 0.0026259129 6.727117e-03 2.051367e-02 11 74250954 74251018 65 + 2.406 2.527 0.401
ENSG00000214517 E025 8.8013432 0.0055666470 1.756819e-01 2.955757e-01 11 74251019 74251647 629 + 1.056 0.937 -0.441
ENSG00000214517 E026 313.1964237 0.0014082734 2.870922e-02 6.942124e-02 11 74251648 74251715 68 + 2.410 2.504 0.315
ENSG00000214517 E027 1911.5592433 0.0024010679 1.764652e-09 2.498192e-08 11 74253492 74254703 1212 + 3.149 3.304 0.513