ENSG00000214135

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000414207 ENSG00000214135 HEK293_OSMI2_2hA HEK293_TMG_2hB SDHAP4 transcribed_unprocessed_pseudogene processed_transcript 8.31031 6.413623 9.166633 0.1542931 0.4143709 0.5145777 0.4523819 0.2858065 0.4283230 0.09779405 0.1345368 0.5673406 0.05371667 0.0442000 0.04556667 0.001366667 1.000000e+00 3.018153e-05 TRUE TRUE
ENST00000418868 ENSG00000214135 HEK293_OSMI2_2hA HEK293_TMG_2hB SDHAP4 transcribed_unprocessed_pseudogene processed_transcript 8.31031 6.413623 9.166633 0.1542931 0.4143709 0.5145777 0.5830439 0.6375541 0.2886253 0.31884481 0.2886253 -1.1166641 0.08091250 0.1003333 0.03406667 -0.066266667 5.410048e-01 3.018153e-05 TRUE TRUE
ENST00000437008 ENSG00000214135 HEK293_OSMI2_2hA HEK293_TMG_2hB SDHAP4 transcribed_unprocessed_pseudogene processed_transcript 8.31031 6.413623 9.166633 0.1542931 0.4143709 0.5145777 0.5892587 0.1852187 1.0482616 0.18521872 0.6241873 2.4385330 0.06926250 0.0303000 0.11146667 0.081166667 5.839977e-01 3.018153e-05 FALSE FALSE
ENST00000449003 ENSG00000214135 HEK293_OSMI2_2hA HEK293_TMG_2hB SDHAP4 transcribed_unprocessed_pseudogene retained_intron 8.31031 6.413623 9.166633 0.1542931 0.4143709 0.5145777 2.2208799 0.9338148 2.7751097 0.09167275 0.1846337 1.5611585 0.25352917 0.1451333 0.30220000 0.157066667 3.018153e-05 3.018153e-05 FALSE TRUE
ENST00000686003 ENSG00000214135 HEK293_OSMI2_2hA HEK293_TMG_2hB SDHAP4 transcribed_unprocessed_pseudogene processed_transcript 8.31031 6.413623 9.166633 0.1542931 0.4143709 0.5145777 1.4731255 1.4132150 2.1735131 0.43137201 0.3017244 0.6174976 0.17176250 0.2180667 0.23513333 0.017066667 9.001695e-01 3.018153e-05 TRUE FALSE
MSTRG.24366.12 ENSG00000214135 HEK293_OSMI2_2hA HEK293_TMG_2hB SDHAP4 transcribed_unprocessed_pseudogene   8.31031 6.413623 9.166633 0.1542931 0.4143709 0.5145777 0.8568485 1.2630486 0.5497704 0.26839921 0.1847595 -1.1853805 0.11057083 0.1962333 0.06020000 -0.136033333 5.659922e-02 3.018153e-05 TRUE FALSE
MSTRG.24366.8 ENSG00000214135 HEK293_OSMI2_2hA HEK293_TMG_2hB SDHAP4 transcribed_unprocessed_pseudogene   8.31031 6.413623 9.166633 0.1542931 0.4143709 0.5145777 0.1333807 0.0000000 0.5823251 0.00000000 0.3032314 5.8883172 0.01511667 0.0000000 0.06670000 0.066700000 2.431163e-01 3.018153e-05 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000214135 E001 0.6224193 0.0180339167 8.854140e-01 9.306994e-01 3 197578072 197578171 100 - 0.204 0.232 0.231
ENSG00000214135 E002 0.0000000       3 197578213 197578709 497 -      
ENSG00000214135 E003 0.5149111 0.0215504908 4.855178e-01 6.233914e-01 3 197579129 197579202 74 - 0.114 0.232 1.233
ENSG00000214135 E004 0.6966155 0.0168514110 2.488152e-01 3.845430e-01 3 197579203 197579243 41 - 0.114 0.314 1.818
ENSG00000214135 E005 0.8438645 0.0144073806 5.435340e-01 6.741182e-01 3 197579244 197579250 7 - 0.204 0.314 0.818
ENSG00000214135 E006 1.1468953 0.0113260926 8.557363e-01 9.108037e-01 3 197579251 197579299 49 - 0.343 0.314 -0.182
ENSG00000214135 E007 0.0000000       3 197579300 197579317 18 -      
ENSG00000214135 E008 0.6277178 0.0192026719 3.893140e-01 5.334237e-01 3 197580697 197580748 52 - 0.279 0.131 -1.352
ENSG00000214135 E009 1.0642801 0.0128222818 3.413544e-01 4.852520e-01 3 197590363 197590366 4 - 0.398 0.232 -1.090
ENSG00000214135 E010 1.2459845 0.0158699957 6.194450e-01 7.368533e-01 3 197590367 197590393 27 - 0.398 0.313 -0.505
ENSG00000214135 E011 2.1369792 0.0065209349 1.784546e-01 2.992148e-01 3 197593091 197593159 69 - 0.602 0.382 -1.090
ENSG00000214135 E012 0.2955422 0.0286276460 9.167589e-01   3 197600272 197600280 9 - 0.114 0.131 0.230
ENSG00000214135 E013 1.1426289 0.0111932513 8.568540e-01 9.115323e-01 3 197600281 197600373 93 - 0.343 0.314 -0.182
ENSG00000214135 E014 10.3429385 0.0274462782 3.629662e-02 8.414756e-02 3 197602974 197603077 104 - 0.898 1.161 0.965
ENSG00000214135 E015 7.9332217 0.1341683283 1.040267e-01 1.966369e-01 3 197603078 197603092 15 - 0.764 1.073 1.173
ENSG00000214135 E016 7.2116250 0.0025901644 3.472183e-04 1.569054e-03 3 197603122 197603154 33 - 0.634 1.077 1.728
ENSG00000214135 E017 11.8330779 0.0062992768 8.191557e-05 4.370468e-04 3 197603529 197603717 189 - 0.845 1.263 1.528
ENSG00000214135 E018 1.0277484 0.0720660841 9.123067e-02 1.770548e-01 3 197608122 197608248 127 - 0.447 0.131 -2.357
ENSG00000214135 E019 1.5574067 0.1845701624 9.610392e-01 9.794613e-01 3 197609446 197609623 178 - 0.399 0.388 -0.057
ENSG00000214135 E020 0.5997190 0.1930210495 8.272173e-02 1.638135e-01 3 197611531 197611532 2 - 0.343 0.001 -9.950
ENSG00000214135 E021 7.5379257 0.0028919675 6.986886e-03 2.119018e-02 3 197611533 197611571 39 - 0.741 1.064 1.232
ENSG00000214135 E022 11.4105005 0.0119093639 6.282833e-04 2.636710e-03 3 197611572 197611622 51 - 0.864 1.251 1.416
ENSG00000214135 E023 56.4352088 0.0005759375 4.544103e-03 1.466528e-02 3 197613225 197613318 94 - 1.688 1.823 0.458
ENSG00000214135 E024 13.8478143 0.0104859783 7.667091e-01 8.491169e-01 3 197614024 197614026 3 - 1.188 1.167 -0.074
ENSG00000214135 E025 16.1675144 0.0034502742 8.622332e-01 9.151566e-01 3 197614027 197614049 23 - 1.243 1.236 -0.027
ENSG00000214135 E026 47.7579855 0.0005619299 1.127548e-01 2.095660e-01 3 197614050 197614285 236 - 1.729 1.657 -0.242
ENSG00000214135 E027 70.7368280 0.0003610327 1.112795e-02 3.147208e-02 3 197614286 197614622 337 - 1.903 1.807 -0.323
ENSG00000214135 E028 17.5046636 0.0027689759 6.878157e-03 2.090728e-02 3 197614623 197614726 104 - 1.360 1.137 -0.786
ENSG00000214135 E029 16.4744841 0.0012011766 1.029219e-01 1.949221e-01 3 197614727 197614842 116 - 1.299 1.169 -0.459
ENSG00000214135 E030 37.8843544 0.0006173462 5.021880e-10 7.856153e-09 3 197614843 197615563 721 - 1.736 1.385 -1.199
ENSG00000214135 E031 56.2541015 0.0068843368 8.279375e-01 8.918332e-01 3 197615564 197615685 122 - 1.762 1.754 -0.025
ENSG00000214135 E032 67.5563038 0.0110415750 7.630851e-01 8.465383e-01 3 197619740 197619888 149 - 1.844 1.831 -0.045
ENSG00000214135 E033 77.0876918 0.0004820870 8.219936e-01 8.877677e-01 3 197621704 197621864 161 - 1.888 1.903 0.050
ENSG00000214135 E034 35.3745085 0.0060534020 9.084840e-01 9.458648e-01 3 197621865 197621868 4 - 1.558 1.574 0.055
ENSG00000214135 E035 19.1152696 0.0010330237 9.793011e-01 9.911096e-01 3 197622187 197622330 144 - 1.306 1.309 0.013
ENSG00000214135 E036 38.2067337 0.0035685029 1.202540e-01 2.203520e-01 3 197623220 197623382 163 - 1.543 1.643 0.340
ENSG00000214135 E037 19.3230595 0.0488136865 6.024481e-01 7.234303e-01 3 197624112 197624187 76 - 1.286 1.330 0.153
ENSG00000214135 E038 17.0356976 0.0544514516 4.099280e-01 5.535571e-01 3 197624188 197624197 10 - 1.213 1.294 0.287
ENSG00000214135 E039 0.3332198 0.0284604042 9.143890e-01   3 197624198 197624198 1 - 0.114 0.131 0.233
ENSG00000214135 E040 4.0284239 0.0436703868 3.014604e-01 4.432724e-01 3 197624472 197624720 249 - 0.786 0.618 -0.696
ENSG00000214135 E041 6.2375982 0.0232902644 4.732235e-02 1.044048e-01 3 197627572 197627779 208 - 0.973 0.690 -1.108
ENSG00000214135 E042 15.4366470 0.0397353655 2.973435e-01 4.386631e-01 3 197627780 197627786 7 - 1.153 1.265 0.399
ENSG00000214135 E043 16.0310529 0.0400158069 2.686222e-01 4.071041e-01 3 197627787 197627915 129 - 1.162 1.283 0.429