ENSG00000213853

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000359543 ENSG00000213853 HEK293_OSMI2_2hA HEK293_TMG_2hB EMP2 protein_coding protein_coding 22.95822 24.80786 25.88102 1.400735 0.5643301 0.06107309 10.767091 10.812325 12.213526 0.09138129 0.4126476 0.1756499 0.4764083 0.4389000 0.47170000 0.03280000 0.71020928 0.04010923 FALSE TRUE
ENST00000536829 ENSG00000213853 HEK293_OSMI2_2hA HEK293_TMG_2hB EMP2 protein_coding protein_coding 22.95822 24.80786 25.88102 1.400735 0.5643301 0.06107309 4.285194 7.829630 2.584804 0.46106547 1.2932338 -1.5951598 0.1836417 0.3166333 0.09793333 -0.21870000 0.45452578 0.04010923 FALSE TRUE
MSTRG.12023.3 ENSG00000213853 HEK293_OSMI2_2hA HEK293_TMG_2hB EMP2 protein_coding   22.95822 24.80786 25.88102 1.400735 0.5643301 0.06107309 4.083364 2.792915 6.569542 1.13275716 0.1296779 1.2310595 0.1744167 0.1083667 0.25413333 0.14576667 0.04010923 0.04010923   FALSE
MSTRG.12023.5 ENSG00000213853 HEK293_OSMI2_2hA HEK293_TMG_2hB EMP2 protein_coding   22.95822 24.80786 25.88102 1.400735 0.5643301 0.06107309 3.499668 3.092315 4.208087 0.54750891 0.9384142 0.4432435 0.1514875 0.1255000 0.16413333 0.03863333 0.74257484 0.04010923 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000213853 E001 366.598933 0.0053811201 5.202273e-01 6.542105e-01 16 10528422 10529440 1019 - 2.550 2.571 0.069
ENSG00000213853 E002 206.163562 0.0002456924 3.093024e-01 4.515823e-01 16 10529441 10529663 223 - 2.330 2.299 -0.104
ENSG00000213853 E003 420.847390 0.0009137412 6.262190e-01 7.421981e-01 16 10529664 10530824 1161 - 2.630 2.614 -0.054
ENSG00000213853 E004 102.542492 0.0051444963 1.146931e-01 2.123850e-01 16 10530825 10530939 115 - 2.052 1.969 -0.278
ENSG00000213853 E005 80.457258 0.0096706198 5.357763e-02 1.155117e-01 16 10530940 10531042 103 - 1.967 1.845 -0.412
ENSG00000213853 E006 26.660707 0.0007994714 7.466973e-01 8.343875e-01 16 10531043 10531053 11 - 1.449 1.421 -0.096
ENSG00000213853 E007 59.845132 0.0003974812 8.334446e-04 3.378414e-03 16 10531054 10531162 109 - 1.860 1.703 -0.532
ENSG00000213853 E008 38.920598 0.0005229007 4.844550e-05 2.739167e-04 16 10531163 10531204 42 - 1.711 1.478 -0.795
ENSG00000213853 E009 76.370926 0.0004170070 2.438342e-05 1.483228e-04 16 10531205 10531414 210 - 1.974 1.797 -0.596
ENSG00000213853 E010 33.011439 0.0043455565 4.337970e-01 5.762031e-01 16 10531415 10531560 146 - 1.563 1.504 -0.201
ENSG00000213853 E011 77.446735 0.0003538956 6.231583e-03 1.922258e-02 16 10531561 10531715 155 - 1.953 1.838 -0.387
ENSG00000213853 E012 404.424932 0.0031027126 1.567554e-02 4.201234e-02 16 10531716 10532848 1133 - 2.644 2.570 -0.249
ENSG00000213853 E013 239.676401 0.0002147297 1.038171e-06 8.584079e-06 16 10532849 10533092 244 - 2.314 2.421 0.354
ENSG00000213853 E014 242.560379 0.0001892400 8.619537e-08 8.858335e-07 16 10537928 10538074 147 - 2.313 2.429 0.386
ENSG00000213853 E015 193.191671 0.0002293168 3.677276e-03 1.223117e-02 16 10543570 10543660 91 - 2.242 2.310 0.227
ENSG00000213853 E016 8.876835 0.0018720818 8.146395e-01 8.825694e-01 16 10544180 10547539 3360 - 1.006 0.975 -0.115
ENSG00000213853 E017 207.872329 0.0015338085 3.121984e-02 7.432804e-02 16 10547540 10547677 138 - 2.279 2.337 0.193
ENSG00000213853 E018 0.000000       16 10558994 10559154 161 -      
ENSG00000213853 E019 125.510475 0.0024389624 9.808485e-02 1.876029e-01 16 10580549 10580632 84 - 2.061 2.119 0.193
ENSG00000213853 E020 2.817215 0.0055517203 3.756877e-07 3.405077e-06 16 10846003 10846046 44 - 0.871 0.000 -12.953