ENSG00000213445

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000394224 ENSG00000213445 HEK293_OSMI2_2hA HEK293_TMG_2hB SIPA1 protein_coding protein_coding 11.49946 23.54172 5.670329 0.7204748 0.08359967 -2.051786 0.6825903 0.3785815 0.4342216 0.1612596 0.10066610 0.1930623 0.08314167 0.01643333 0.07710000 0.06066667 4.996507e-02 1.594974e-12 FALSE TRUE
ENST00000526137 ENSG00000213445 HEK293_OSMI2_2hA HEK293_TMG_2hB SIPA1 protein_coding protein_coding 11.49946 23.54172 5.670329 0.7204748 0.08359967 -2.051786 0.4837538 0.6907410 0.4079173 0.1975543 0.10021104 -0.7456639 0.05526250 0.02923333 0.07240000 0.04316667 2.118870e-01 1.594974e-12 FALSE FALSE
ENST00000527525 ENSG00000213445 HEK293_OSMI2_2hA HEK293_TMG_2hB SIPA1 protein_coding protein_coding 11.49946 23.54172 5.670329 0.7204748 0.08359967 -2.051786 0.7842790 0.5270669 0.6018639 0.1072864 0.10576321 0.1881090 0.09800833 0.02263333 0.10563333 0.08300000 9.568321e-05 1.594974e-12 FALSE TRUE
ENST00000528699 ENSG00000213445 HEK293_OSMI2_2hA HEK293_TMG_2hB SIPA1 protein_coding retained_intron 11.49946 23.54172 5.670329 0.7204748 0.08359967 -2.051786 0.7863835 1.4761991 0.3533832 0.5403029 0.01600544 -2.0320638 0.06987917 0.06150000 0.06240000 0.00090000 9.351539e-01 1.594974e-12   FALSE
ENST00000529725 ENSG00000213445 HEK293_OSMI2_2hA HEK293_TMG_2hB SIPA1 protein_coding retained_intron 11.49946 23.54172 5.670329 0.7204748 0.08359967 -2.051786 1.7604996 6.5497018 0.3209669 1.3431055 0.32096691 -4.3088713 0.10492917 0.28006667 0.05523333 -0.22483333 1.031720e-01 1.594974e-12 FALSE TRUE
ENST00000534313 ENSG00000213445 HEK293_OSMI2_2hA HEK293_TMG_2hB SIPA1 protein_coding protein_coding 11.49946 23.54172 5.670329 0.7204748 0.08359967 -2.051786 5.5561157 9.6109024 2.8463155 1.7643445 0.14001358 -1.7520169 0.45998750 0.40626667 0.50216667 0.09590000 5.901141e-01 1.594974e-12 FALSE TRUE
MSTRG.5728.3 ENSG00000213445 HEK293_OSMI2_2hA HEK293_TMG_2hB SIPA1 protein_coding   11.49946 23.54172 5.670329 0.7204748 0.08359967 -2.051786 0.6137668 3.5693797 0.0000000 0.5687536 0.00000000 -8.4835658 0.03890417 0.15220000 0.00000000 -0.15220000 1.594974e-12 1.594974e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000213445 E001 1.3974853 0.0106895818 5.163348e-02 0.1120636093 11 65638101 65638110 10 + 0.568 0.212 -2.096
ENSG00000213445 E002 3.3723611 0.0489036086 4.729370e-01 0.6119561809 11 65638111 65638112 2 + 0.640 0.509 -0.597
ENSG00000213445 E003 13.1275184 0.0212321417 4.331809e-01 0.5756147167 11 65638113 65638135 23 + 1.095 0.998 -0.355
ENSG00000213445 E004 21.9819558 0.0194201556 7.583630e-01 0.8429978048 11 65638136 65638151 16 + 1.251 1.222 -0.104
ENSG00000213445 E005 29.6124759 0.0324164282 2.116573e-01 0.3404441235 11 65638152 65638182 31 + 1.457 1.329 -0.441
ENSG00000213445 E006 8.0418747 0.0252206045 3.228828e-02 0.0764089433 11 65638183 65638420 238 + 1.071 0.771 -1.136
ENSG00000213445 E007 5.1552679 0.0042311704 1.108481e-03 0.0043314697 11 65639136 65639251 116 + 1.018 0.565 -1.818
ENSG00000213445 E008 6.7670522 0.0085407481 9.494228e-05 0.0004982557 11 65639866 65640021 156 + 1.140 0.635 -1.950
ENSG00000213445 E009 6.0586937 0.0058256745 2.308096e-03 0.0081769416 11 65640121 65640295 175 + 1.045 0.634 -1.612
ENSG00000213445 E010 2.9378766 0.0073345436 3.091224e-01 0.4513820955 11 65640801 65640830 30 + 0.640 0.461 -0.835
ENSG00000213445 E011 227.0802596 0.0069599119 2.053585e-01 0.3328703397 11 65640831 65641600 770 + 2.268 2.214 -0.182
ENSG00000213445 E012 0.1482932 0.0415459320 1.000000e+00   11 65642118 65642249 132 + 0.000 0.063 7.232
ENSG00000213445 E013 89.8149495 0.0003722134 4.994746e-01 0.6357941601 11 65642250 65642377 128 + 1.847 1.813 -0.115
ENSG00000213445 E014 84.1603056 0.0042208504 5.590553e-01 0.6872231445 11 65642463 65642603 141 + 1.760 1.796 0.122
ENSG00000213445 E015 58.3670813 0.0017748654 7.411864e-01 0.8304484545 11 65642604 65642639 36 + 1.617 1.637 0.066
ENSG00000213445 E016 121.9264558 0.0002933162 8.645888e-01 0.9167184356 11 65644955 65645129 175 + 1.947 1.952 0.017
ENSG00000213445 E017 0.2966881 0.0290785164 1.805605e-02   11 65645811 65645853 43 + 0.371 0.000 -12.334
ENSG00000213445 E018 103.9674454 0.0003050027 8.560949e-01 0.9110508091 11 65645854 65645957 104 + 1.879 1.886 0.020
ENSG00000213445 E019 116.1367121 0.0002903979 5.131448e-01 0.6479874660 11 65646221 65646378 158 + 1.957 1.928 -0.098
ENSG00000213445 E020 94.6257803 0.0003455359 7.805268e-01 0.8589712436 11 65646456 65646663 208 + 1.856 1.841 -0.051
ENSG00000213445 E021 46.1708406 0.0028537405 8.258857e-01 0.8903976744 11 65646664 65646872 209 + 1.548 1.532 -0.056
ENSG00000213445 E022 29.9143118 0.0103055171 4.172976e-02 0.0942419169 11 65646873 65646969 97 + 1.496 1.317 -0.618
ENSG00000213445 E023 35.9782430 0.0095486627 2.447241e-03 0.0086045644 11 65646970 65647065 96 + 1.624 1.382 -0.833
ENSG00000213445 E024 0.0000000       11 65647366 65647383 18 +      
ENSG00000213445 E025 65.8206748 0.0121134013 3.426053e-03 0.0115133832 11 65647384 65647658 275 + 1.856 1.650 -0.695
ENSG00000213445 E026 137.5212393 0.0026585342 1.975696e-01 0.3231785948 11 65649262 65649480 219 + 1.960 2.019 0.196
ENSG00000213445 E027 1.9165611 0.0075613074 5.090676e-01 0.6444156620 11 65649512 65649560 49 + 0.480 0.354 -0.683
ENSG00000213445 E028 152.9936011 0.0023757561 2.825720e-02 0.0685171017 11 65649561 65649672 112 + 1.973 2.070 0.324
ENSG00000213445 E029 0.8908930 0.2675094640 1.380141e-01 0.2453104656 11 65649686 65649756 71 + 0.480 0.163 -2.154
ENSG00000213445 E030 166.4334298 0.0013694271 1.323821e-01 0.2374045241 11 65649757 65649865 109 + 2.042 2.100 0.195
ENSG00000213445 E031 162.9042358 0.0006833125 5.350198e-01 0.6669730436 11 65649950 65650051 102 + 2.063 2.084 0.072
ENSG00000213445 E032 119.4152153 0.0002739996 8.723346e-01 0.9218973946 11 65650138 65650192 55 + 1.941 1.945 0.015
ENSG00000213445 E033 6.6269563 0.0025991319 9.882192e-01 0.9967001396 11 65650282 65650400 119 + 0.757 0.757 0.002
ENSG00000213445 E034 154.1425407 0.0004436926 8.633897e-03 0.0253709611 11 65650401 65650479 79 + 1.970 2.070 0.337
ENSG00000213445 E035 191.6856150 0.0090402301 6.258470e-03 0.0192901272 11 65650569 65650993 425 + 2.017 2.175 0.528