ENSG00000213281

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369535 ENSG00000213281 HEK293_OSMI2_2hA HEK293_TMG_2hB NRAS protein_coding protein_coding 33.52826 8.745771 66.21579 2.206048 2.200328 2.919087 23.792577 8.745771 44.500500 2.206048 2.4060041 2.345840 0.83660417 1 0.6713000 -0.3287000 9.394853e-39 9.394853e-39 FALSE TRUE
MSTRG.1872.3 ENSG00000213281 HEK293_OSMI2_2hA HEK293_TMG_2hB NRAS protein_coding   33.52826 8.745771 66.21579 2.206048 2.200328 2.919087 3.507641 0.000000 8.357586 0.000000 0.5636974 9.708668 0.06128750 0 0.1260667 0.1260667 4.132116e-24 9.394853e-39   FALSE
MSTRG.1872.4 ENSG00000213281 HEK293_OSMI2_2hA HEK293_TMG_2hB NRAS protein_coding   33.52826 8.745771 66.21579 2.206048 2.200328 2.919087 5.719885 0.000000 11.449582 0.000000 0.1271650 10.162339 0.09402917 0 0.1732667 0.1732667 1.293612e-32 9.394853e-39 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000213281 E001 1430.7134 0.0088576180 3.218183e-14 9.827129e-13 1 114704469 114706669 2201 - 2.923 3.295 1.235
ENSG00000213281 E002 456.5370 0.0001326900 8.163757e-03 2.419770e-02 1 114706670 114707244 575 - 2.489 2.592 0.342
ENSG00000213281 E003 711.5545 0.0108962747 1.035790e-02 2.964472e-02 1 114707245 114708050 806 - 2.694 2.705 0.035
ENSG00000213281 E004 113.6472 0.0015608486 7.011455e-03 2.125381e-02 1 114708154 114708192 39 - 1.901 1.921 0.066
ENSG00000213281 E005 235.0120 0.0009603594 3.646818e-08 4.037629e-07 1 114708531 114708654 124 - 2.223 2.183 -0.136
ENSG00000213281 E006 329.2955 0.0001756753 2.312356e-17 1.075544e-15 1 114709569 114709728 160 - 2.373 2.292 -0.270
ENSG00000213281 E007 291.2235 0.0001504872 4.194865e-17 1.886315e-15 1 114713800 114713978 179 - 2.322 2.227 -0.318
ENSG00000213281 E008 234.6632 0.0001764916 1.112298e-18 6.104329e-17 1 114716050 114716177 128 - 2.234 2.085 -0.499
ENSG00000213281 E009 154.6877 0.0002548600 7.198580e-13 1.806530e-11 1 114716658 114716778 121 - 2.055 1.902 -0.511