ENSG00000212747

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000391440 ENSG00000212747 HEK293_OSMI2_2hA HEK293_TMG_2hB RTL8B protein_coding protein_coding 10.47892 19.40849 6.60639 0.4353604 0.07277187 -1.553315 10.1547238 19.2300145 6.0638511 0.51135298 0.1602575 -1.663426 0.95362917 0.990633333 0.91773333 -0.0729 0.0002813172 0.0002813172 FALSE  
MSTRG.34919.1 ENSG00000212747 HEK293_OSMI2_2hA HEK293_TMG_2hB RTL8B protein_coding   10.47892 19.40849 6.60639 0.4353604 0.07277187 -1.553315 0.3241937 0.1784755 0.5425394 0.07616763 0.1216498 1.551700 0.04637083 0.009366667 0.08226667 0.0729 0.0002929239 0.0002813172 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000212747 E001 3.870769 0.009618301 4.386569e-09 5.775400e-08 X 135016878 135017064 187 - 1.107 0.278 -3.718
ENSG00000212747 E002 3.945896 0.074266014 5.166556e-05 2.900372e-04 X 135017639 135017751 113 - 1.065 0.354 -3.075
ENSG00000212747 E003 3.952163 0.069374902 4.602787e-04 2.007561e-03 X 135018107 135018200 94 - 1.019 0.440 -2.428
ENSG00000212747 E004 4.159315 0.153934387 2.592629e-04 1.213774e-03 X 135019068 135019149 82 - 1.109 0.349 -3.262
ENSG00000212747 E005 427.114512 0.003798025 6.457364e-01 7.578072e-01 X 135020513 135021394 882 - 2.534 2.537 0.008
ENSG00000212747 E006 926.245089 0.002859091 1.466293e-02 3.973235e-02 X 135021395 135023877 2483 - 2.834 2.879 0.149