ENSG00000206527

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000383657 ENSG00000206527 HEK293_OSMI2_2hA HEK293_TMG_2hB HACD2 protein_coding protein_coding 21.37139 6.469496 33.87975 0.9974743 0.6425566 2.386895 19.4997509 6.015363 29.385136 0.9902554 0.8029138 2.286458 0.90685417 0.9284667 0.8670667 -0.0614 1.689588e-01 7.765274e-13 FALSE TRUE
MSTRG.23552.3 ENSG00000206527 HEK293_OSMI2_2hA HEK293_TMG_2hB HACD2 protein_coding   21.37139 6.469496 33.87975 0.9974743 0.6425566 2.386895 0.9645414 0.000000 3.673124 0.0000000 0.3975489 8.524786 0.02821667 0.0000000 0.1086000 0.1086 7.765274e-13 7.765274e-13 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000206527 E001 0.2214452 0.0537286792 7.995393e-02   3 123490820 123491114 295 - 0.000 0.282 15.669
ENSG00000206527 E002 0.2214452 0.0537286792 7.995393e-02   3 123491115 123491184 70 - 0.000 0.282 15.681
ENSG00000206527 E003 1399.2664534 0.0202387374 4.547269e-06 3.267958e-05 3 123491554 123494792 3239 - 2.920 3.243 1.075
ENSG00000206527 E004 149.0302566 0.0038220518 1.745286e-03 6.425169e-03 3 123494793 123494970 178 - 2.005 2.049 0.146
ENSG00000206527 E005 3.0019257 0.0975273793 4.631419e-01 6.029677e-01 3 123499291 123499582 292 - 0.491 0.449 -0.211
ENSG00000206527 E006 2.4345978 0.0062594424 1.733021e-01 2.924964e-01 3 123499583 123499630 48 - 0.358 0.744 1.828
ENSG00000206527 E007 1.6951184 0.0080108836 1.312096e-01 2.358375e-01 3 123499631 123499640 10 - 0.259 0.666 2.158
ENSG00000206527 E008 104.1184740 0.0005024913 1.096327e-04 5.662990e-04 3 123500515 123500567 53 - 1.856 1.873 0.058
ENSG00000206527 E009 143.0063379 0.0002280385 4.145029e-08 4.545663e-07 3 123500568 123500693 126 - 1.998 1.976 -0.074
ENSG00000206527 E010 154.9314047 0.0003208528 2.012609e-09 2.814853e-08 3 123502560 123502681 122 - 2.034 1.997 -0.126
ENSG00000206527 E011 0.1515154 0.0438510204 1.000000e+00   3 123502682 123502906 225 - 0.048 0.000 -10.018
ENSG00000206527 E012 1.4843684 0.0340885362 6.555538e-02 1.359690e-01 3 123513578 123513723 146 - 0.199 0.667 2.645
ENSG00000206527 E013 108.9675897 0.0012911291 3.498617e-08 3.886721e-07 3 123528386 123528436 51 - 1.888 1.804 -0.284
ENSG00000206527 E014 96.3174768 0.0005731381 3.051299e-10 4.959330e-09 3 123528437 123528474 38 - 1.842 1.700 -0.479
ENSG00000206527 E015 76.2995642 0.0033256588 1.286667e-05 8.332374e-05 3 123567762 123567780 19 - 1.736 1.649 -0.293
ENSG00000206527 E016 117.4510009 0.0002547081 1.874717e-07 1.806233e-06 3 123582212 123582329 118 - 1.915 1.878 -0.121
ENSG00000206527 E017 0.4502799 0.0297373952 8.521673e-01 9.083676e-01 3 123584402 123584555 154 - 0.130 0.000 -11.603
ENSG00000206527 E018 69.5194794 0.0012877759 1.197936e-02 3.348216e-02 3 123584873 123585082 210 - 1.677 1.730 0.180