ENSG00000205309

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000389022 ENSG00000205309 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5M protein_coding protein_coding 7.399297 14.24466 4.999552 0.8099125 0.2617082 -1.50868 0.3930460 0.0000000 0.0000000 0.0000000 0.00000000 0.0000000 0.05212500 0.00000000 0.0000000 0.00000000   0.002110627 FALSE TRUE
ENST00000478373 ENSG00000205309 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5M protein_coding nonsense_mediated_decay 7.399297 14.24466 4.999552 0.8099125 0.2617082 -1.50868 1.1987065 1.7200166 0.9446342 0.4025671 0.20249306 -0.8577660 0.16955833 0.12083333 0.1857000 0.06486667 0.453405731 0.002110627 TRUE FALSE
ENST00000483704 ENSG00000205309 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5M protein_coding nonsense_mediated_decay 7.399297 14.24466 4.999552 0.8099125 0.2617082 -1.50868 0.5181667 1.3732433 0.0637971 0.2715006 0.06379710 -4.2283470 0.06416250 0.09930000 0.0127000 -0.08660000 0.052594381 0.002110627 FALSE TRUE
ENST00000582909 ENSG00000205309 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5M protein_coding processed_transcript 7.399297 14.24466 4.999552 0.8099125 0.2617082 -1.50868 0.3485277 1.9488006 0.0000000 1.0451083 0.00000000 -7.6138267 0.03909167 0.12906667 0.0000000 -0.12906667 0.250269341 0.002110627 FALSE TRUE
ENST00000616989 ENSG00000205309 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5M protein_coding protein_coding 7.399297 14.24466 4.999552 0.8099125 0.2617082 -1.50868 1.0559471 1.9538406 0.8623761 0.2670972 0.06660371 -1.1706556 0.13352083 0.13950000 0.1720667 0.03256667 0.650379826 0.002110627 FALSE TRUE
MSTRG.13760.5 ENSG00000205309 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5M protein_coding   7.399297 14.24466 4.999552 0.8099125 0.2617082 -1.50868 0.6470430 0.6373739 0.9077357 0.3251850 0.26874660 0.5034795 0.09971667 0.04666667 0.1882000 0.14153333 0.340190695 0.002110627 FALSE TRUE
MSTRG.13760.6 ENSG00000205309 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5M protein_coding   7.399297 14.24466 4.999552 0.8099125 0.2617082 -1.50868 0.4159581 1.3147603 0.0000000 0.5220635 0.00000000 -7.0495875 0.04445833 0.09330000 0.0000000 -0.09330000 0.002110627 0.002110627 FALSE TRUE
MSTRG.13760.7 ENSG00000205309 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5M protein_coding   7.399297 14.24466 4.999552 0.8099125 0.2617082 -1.50868 0.2595826 0.0000000 0.5413888 0.0000000 0.30933544 5.7849980 0.04282917 0.00000000 0.1063000 0.10630000 0.092989234 0.002110627 FALSE TRUE
MSTRG.13760.9 ENSG00000205309 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5M protein_coding   7.399297 14.24466 4.999552 0.8099125 0.2617082 -1.50868 2.1559907 4.5949232 1.4197573 0.3789190 0.17046701 -1.6874068 0.30640417 0.32286667 0.2832000 -0.03966667 0.710195043 0.002110627 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000205309 E001 1.586564 0.0093391548 6.465362e-01 7.584041e-01 17 17303335 17303365 31 + 0.414 0.342 -0.416
ENSG00000205309 E002 2.912043 0.0054687614 7.120560e-02 1.453426e-01 17 17303366 17303372 7 + 0.721 0.446 -1.246
ENSG00000205309 E003 4.830881 0.0877971718 2.196516e-01 3.499717e-01 17 17303373 17303389 17 + 0.836 0.634 -0.826
ENSG00000205309 E004 16.640057 0.0849250092 4.050062e-02 9.193952e-02 17 17303390 17303664 275 + 1.379 1.082 -1.052
ENSG00000205309 E005 9.447319 0.0018622390 7.371771e-04 3.033048e-03 17 17303665 17303677 13 + 1.186 0.843 -1.266
ENSG00000205309 E006 38.083326 0.0021291590 4.767494e-02 1.050377e-01 17 17303678 17303817 140 + 1.600 1.495 -0.359
ENSG00000205309 E007 19.303548 0.0022892585 8.817263e-01 9.282096e-01 17 17303818 17303901 84 + 1.229 1.234 0.017
ENSG00000205309 E008 3.844880 0.0044243374 2.967067e-02 7.131554e-02 17 17304342 17304363 22 + 0.836 0.530 -1.292
ENSG00000205309 E009 19.034247 0.0011579308 4.346761e-04 1.907999e-03 17 17304364 17304477 114 + 1.424 1.162 -0.916
ENSG00000205309 E010 1.910221 0.0074967071 2.112789e-02 5.386744e-02 17 17304478 17304481 4 + 0.674 0.300 -1.903
ENSG00000205309 E011 57.162596 0.0004448566 1.281493e-01 2.315807e-01 17 17306543 17306643 101 + 1.738 1.683 -0.188
ENSG00000205309 E012 50.408205 0.0020151294 9.409212e-01 9.668510e-01 17 17323185 17323245 61 + 1.628 1.652 0.081
ENSG00000205309 E013 6.798667 0.0588113694 1.219817e-02 3.400641e-02 17 17333496 17333510 15 + 1.086 0.700 -1.480
ENSG00000205309 E014 10.515659 0.0285108088 8.962176e-02 1.745459e-01 17 17333511 17333588 78 + 1.139 0.935 -0.748
ENSG00000205309 E015 70.865990 0.0040365673 4.195183e-02 9.463853e-02 17 17344794 17344908 115 + 1.684 1.815 0.442
ENSG00000205309 E016 16.607298 0.0060921205 3.629864e-01 5.074095e-01 17 17344909 17344926 18 + 1.085 1.198 0.403
ENSG00000205309 E017 1.404772 0.1702787555 6.158195e-01 7.339970e-01 17 17345269 17345312 44 + 0.414 0.335 -0.457
ENSG00000205309 E018 215.733901 0.0047849976 5.890765e-07 5.141616e-06 17 17346805 17347663 859 + 2.118 2.303 0.617