ENSG00000205250

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000379378 ENSG00000205250 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F4 protein_coding protein_coding 104.9672 140.08 86.15708 4.933797 3.434645 -0.7011453 83.511022 125.325783 61.372453 3.7634741 1.5991214 -1.0299002 0.77675000 0.89500000 0.7131333 -0.18186667 7.148191e-22 7.148191e-22 FALSE TRUE
ENST00000561904 ENSG00000205250 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F4 protein_coding retained_intron 104.9672 140.08 86.15708 4.933797 3.434645 -0.7011453 6.246794 4.014898 7.138276 0.6369862 0.5951318 0.8286431 0.06512917 0.02846667 0.0826000 0.05413333 7.385947e-07 7.148191e-22   FALSE
ENST00000568693 ENSG00000205250 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F4 protein_coding retained_intron 104.9672 140.08 86.15708 4.933797 3.434645 -0.7011453 5.116165 2.914228 5.987326 0.2636545 0.7116317 1.0362638 0.05381250 0.02080000 0.0691000 0.04830000 8.364473e-10 7.148191e-22   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000205250 E001 1.689904 2.616724e-02 4.607950e-01 6.007793e-01 16 67186742 67186781 40 + 0.279 0.424 0.879
ENSG00000205250 E002 2.400252 6.219557e-03 4.822703e-01 6.204225e-01 16 67192008 67192115 108 + 0.568 0.460 -0.521
ENSG00000205250 E003 3.890590 4.959527e-02 2.872534e-04 1.328142e-03 16 67192116 67192154 39 + 0.980 0.383 -2.594
ENSG00000205250 E004 5.993102 1.830577e-02 2.070191e-08 2.406525e-07 16 67192155 67192173 19 + 1.188 0.423 -3.127
ENSG00000205250 E005 15.894691 3.984818e-02 9.897791e-06 6.586255e-05 16 67192174 67192184 11 + 1.487 0.948 -1.915
ENSG00000205250 E006 24.976168 4.480329e-02 8.839793e-04 3.557985e-03 16 67192185 67192189 5 + 1.604 1.221 -1.324
ENSG00000205250 E007 99.674042 1.263118e-02 2.214389e-03 7.888034e-03 16 67192190 67192206 17 + 2.080 1.899 -0.610
ENSG00000205250 E008 103.724281 9.935974e-03 2.546136e-03 8.900906e-03 16 67192207 67192207 1 + 2.087 1.923 -0.551
ENSG00000205250 E009 124.123528 1.171852e-02 4.037729e-03 1.324793e-02 16 67192208 67192212 5 + 2.162 2.002 -0.534
ENSG00000205250 E010 164.294571 7.410586e-03 1.058226e-02 3.016875e-02 16 67192213 67192214 2 + 2.254 2.141 -0.377
ENSG00000205250 E011 578.389960 5.475608e-03 7.208658e-03 2.176730e-02 16 67192215 67192362 148 + 2.782 2.697 -0.285
ENSG00000205250 E012 9.052880 1.516459e-02 2.295159e-03 8.135216e-03 16 67192363 67192494 132 + 1.175 0.824 -1.302
ENSG00000205250 E013 7.430728 2.307965e-03 2.139590e-04 1.023893e-03 16 67192495 67192526 32 + 1.134 0.719 -1.575
ENSG00000205250 E014 12.564420 1.393590e-03 2.649950e-09 3.633912e-08 16 67192527 67192664 138 + 1.396 0.852 -1.965
ENSG00000205250 E015 10.780027 2.023372e-03 1.128105e-06 9.263409e-06 16 67192665 67192760 96 + 1.309 0.837 -1.720
ENSG00000205250 E016 627.849514 2.705268e-03 1.803625e-03 6.610778e-03 16 67192761 67192824 64 + 2.810 2.736 -0.246
ENSG00000205250 E017 608.790207 5.655687e-04 2.664425e-05 1.604576e-04 16 67192825 67192870 46 + 2.789 2.725 -0.214
ENSG00000205250 E018 79.379861 4.124229e-03 3.085353e-16 1.250023e-14 16 67192871 67193008 138 + 2.100 1.702 -1.337
ENSG00000205250 E019 948.918207 3.144523e-04 4.313909e-02 9.680273e-02 16 67193009 67193170 162 + 2.950 2.934 -0.052
ENSG00000205250 E020 24.238358 7.961152e-04 1.928647e-11 3.833155e-10 16 67193171 67193421 251 + 1.628 1.172 -1.578
ENSG00000205250 E021 15.392740 1.192996e-03 4.833586e-08 5.228735e-07 16 67193422 67193471 50 + 1.440 0.987 -1.610
ENSG00000205250 E022 430.079829 2.410639e-04 8.384883e-03 2.475095e-02 16 67193472 67193515 44 + 2.550 2.615 0.215
ENSG00000205250 E023 58.832042 4.255593e-04 2.694849e-23 2.463953e-21 16 67193978 67194397 420 + 1.996 1.551 -1.506
ENSG00000205250 E024 584.710694 8.501699e-04 1.166278e-01 2.151432e-01 16 67194398 67194459 62 + 2.697 2.744 0.154
ENSG00000205250 E025 1300.327626 6.197219e-04 3.714403e-01 5.158887e-01 16 67194686 67194951 266 + 3.058 3.085 0.090
ENSG00000205250 E026 419.857530 1.872525e-03 9.835393e-02 1.880151e-01 16 67194952 67194980 29 + 2.544 2.604 0.198
ENSG00000205250 E027 22.400085 9.527841e-04 2.056019e-07 1.966113e-06 16 67195651 67195781 131 + 1.547 1.180 -1.277
ENSG00000205250 E028 1076.778569 9.097691e-05 7.692795e-03 2.300661e-02 16 67195782 67196006 225 + 2.962 3.006 0.148
ENSG00000205250 E029 1.176027 1.861133e-02 2.146083e-01 3.439873e-01 16 67196007 67196008 2 + 0.447 0.232 -1.350
ENSG00000205250 E030 662.326212 9.433602e-05 8.085557e-02 1.608764e-01 16 67197599 67197646 48 + 2.755 2.794 0.130
ENSG00000205250 E031 683.906596 1.173537e-04 3.600088e-01 5.043193e-01 16 67197867 67197911 45 + 2.777 2.803 0.088
ENSG00000205250 E032 20.636935 9.115604e-04 7.557745e-05 4.070135e-04 16 67197912 67198007 96 + 1.475 1.186 -1.006
ENSG00000205250 E033 1102.163538 1.026691e-03 4.448821e-02 9.926692e-02 16 67198008 67198130 123 + 2.968 3.020 0.172
ENSG00000205250 E034 2443.958134 2.427125e-03 6.152504e-08 6.511583e-07 16 67198131 67198918 788 + 3.255 3.387 0.437