ENSG00000204991

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000378247 ENSG00000204991 HEK293_OSMI2_2hA HEK293_TMG_2hB SPIRE2 protein_coding protein_coding 9.860937 13.68534 8.358217 1.147347 0.2830672 -0.7106933 3.9972912 5.1881230 4.17326462 0.4671776 0.37051822 -0.3133615 0.40102917 0.38216667 0.497800000 0.11563333 0.21119980 0.001356307 FALSE  
ENST00000561883 ENSG00000204991 HEK293_OSMI2_2hA HEK293_TMG_2hB SPIRE2 protein_coding retained_intron 9.860937 13.68534 8.358217 1.147347 0.2830672 -0.7106933 0.5909547 0.5841647 0.70553474 0.1098568 0.02260950 0.2681590 0.06462500 0.04196667 0.084733333 0.04276667 0.02028279 0.001356307 FALSE  
ENST00000562029 ENSG00000204991 HEK293_OSMI2_2hA HEK293_TMG_2hB SPIRE2 protein_coding retained_intron 9.860937 13.68534 8.358217 1.147347 0.2830672 -0.7106933 1.4302500 1.6163778 1.33470744 0.1807014 0.08921691 -0.2743701 0.14842500 0.11773333 0.159633333 0.04190000 0.22788867 0.001356307 FALSE  
ENST00000564878 ENSG00000204991 HEK293_OSMI2_2hA HEK293_TMG_2hB SPIRE2 protein_coding processed_transcript 9.860937 13.68534 8.358217 1.147347 0.2830672 -0.7106933 0.7765954 0.8593444 0.70888502 0.1331087 0.14391524 -0.2741668 0.08252917 0.06266667 0.084600000 0.02193333 0.65588975 0.001356307 FALSE  
ENST00000565628 ENSG00000204991 HEK293_OSMI2_2hA HEK293_TMG_2hB SPIRE2 protein_coding retained_intron 9.860937 13.68534 8.358217 1.147347 0.2830672 -0.7106933 0.3532733 1.1517323 0.04014736 0.5719150 0.04014736 -4.5339600 0.03167917 0.08566667 0.005033333 -0.08063333 0.04908881 0.001356307 FALSE  
ENST00000566337 ENSG00000204991 HEK293_OSMI2_2hA HEK293_TMG_2hB SPIRE2 protein_coding nonsense_mediated_decay 9.860937 13.68534 8.358217 1.147347 0.2830672 -0.7106933 0.1845121 0.0000000 0.43958384 0.0000000 0.22108987 5.4905183 0.02170417 0.00000000 0.052100000 0.05210000 0.12770984 0.001356307 FALSE  
MSTRG.13272.8 ENSG00000204991 HEK293_OSMI2_2hA HEK293_TMG_2hB SPIRE2 protein_coding   9.860937 13.68534 8.358217 1.147347 0.2830672 -0.7106933 1.0664251 2.3399105 0.34655279 0.3561579 0.34655279 -2.7204182 0.10498333 0.16983333 0.042200000 -0.12763333 0.12683570 0.001356307 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000204991 E001 5.4355076 0.0837515270 8.403437e-01 9.003234e-01 16 89818179 89818249 71 + 0.799 0.777 -0.089
ENSG00000204991 E002 9.5762657 0.0151248222 8.282131e-01 8.920489e-01 16 89818250 89818538 289 + 1.011 0.993 -0.065
ENSG00000204991 E003 0.9943332 0.0145310078 7.735494e-02 1.552368e-01 16 89822233 89822530 298 + 0.001 0.343 9.984
ENSG00000204991 E004 3.4633664 0.0343487121 3.769704e-06 2.759928e-05 16 89828475 89828498 24 + 0.992 0.235 -3.615
ENSG00000204991 E005 5.2463881 0.0524624580 1.596550e-07 1.559563e-06 16 89828499 89828546 48 + 1.167 0.291 -3.838
ENSG00000204991 E006 12.9365840 0.0312723259 2.550906e-04 1.196518e-03 16 89828547 89828648 102 + 1.363 0.917 -1.602
ENSG00000204991 E007 12.9125773 0.0078651797 2.308367e-02 5.792150e-02 16 89828649 89828697 49 + 1.238 1.018 -0.792
ENSG00000204991 E008 27.4160715 0.0007350010 1.895335e-02 4.923145e-02 16 89828698 89828794 97 + 1.509 1.359 -0.518
ENSG00000204991 E009 1.1812384 0.0475197753 1.294123e-01 2.333516e-01 16 89832282 89832327 46 + 0.506 0.234 -1.626
ENSG00000204991 E010 9.0378811 0.0134231275 8.490089e-01 9.062910e-01 16 89836215 89836433 219 + 0.929 0.965 0.135
ENSG00000204991 E011 33.5369320 0.0006616082 4.544798e-02 1.010194e-01 16 89845322 89845365 44 + 1.575 1.460 -0.394
ENSG00000204991 E012 19.1626897 0.0020222007 8.342493e-02 1.649268e-01 16 89845626 89847156 1531 + 1.140 1.301 0.572
ENSG00000204991 E013 1.2867575 0.0106835810 2.224228e-02 5.617787e-02 16 89849866 89850303 438 + 0.562 0.170 -2.461
ENSG00000204991 E014 91.9548769 0.0004911566 8.069732e-04 3.283435e-03 16 89850304 89850660 357 + 2.005 1.886 -0.398
ENSG00000204991 E015 41.1119433 0.0140904622 3.512922e-01 4.955159e-01 16 89854286 89854321 36 + 1.633 1.570 -0.213
ENSG00000204991 E016 49.6341648 0.0072639994 1.528112e-01 2.654736e-01 16 89854322 89854366 45 + 1.725 1.642 -0.280
ENSG00000204991 E017 91.4380084 0.0014889377 5.802508e-01 7.050158e-01 16 89854487 89854651 165 + 1.935 1.921 -0.047
ENSG00000204991 E018 70.0219448 0.0004016030 1.605899e-01 2.759093e-01 16 89855600 89855686 87 + 1.759 1.831 0.243
ENSG00000204991 E019 7.3353345 0.0023957829 8.288027e-02 1.640606e-01 16 89855687 89856061 375 + 1.011 0.815 -0.746
ENSG00000204991 E020 68.7372942 0.0004112458 2.210450e-01 3.516683e-01 16 89856113 89856236 124 + 1.759 1.823 0.216
ENSG00000204991 E021 64.0980362 0.0004755773 9.883781e-01 9.967952e-01 16 89858338 89858507 170 + 1.766 1.774 0.027
ENSG00000204991 E022 79.1070972 0.0004052821 1.824980e-01 3.043491e-01 16 89859165 89859354 190 + 1.821 1.886 0.218
ENSG00000204991 E023 81.4097000 0.0025631741 8.354923e-02 1.651070e-01 16 89860683 89860795 113 + 1.813 1.904 0.308
ENSG00000204991 E024 65.9290749 0.0057554852 6.186752e-03 1.910544e-02 16 89861966 89863441 1476 + 1.883 1.743 -0.474
ENSG00000204991 E025 6.0681449 0.0030336438 2.416042e-01 3.760783e-01 16 89863442 89863475 34 + 0.906 0.764 -0.554
ENSG00000204991 E026 100.6106027 0.0043720545 1.086218e-01 2.035016e-01 16 89863476 89863610 135 + 1.907 1.995 0.294
ENSG00000204991 E027 77.3573654 0.0020170646 5.531151e-02 1.185295e-01 16 89863794 89863861 68 + 1.779 1.880 0.342
ENSG00000204991 E028 51.7430534 0.0119305227 7.150579e-01 8.110939e-01 16 89868189 89868216 28 + 1.650 1.694 0.149
ENSG00000204991 E029 89.2498642 0.0012032200 4.474979e-01 5.888732e-01 16 89869567 89869682 116 + 1.886 1.927 0.140
ENSG00000204991 E030 319.0598093 0.0070315033 2.857261e-02 6.915444e-02 16 89870050 89871319 1270 + 2.399 2.499 0.334