ENSG00000204859

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000319084 ENSG00000204859 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB48 protein_coding protein_coding 33.81074 50.65671 20.24123 1.017316 0.7433446 -1.323029 1.114982 0.000000 3.0901786 0.0000000 0.8483332 8.2762075 0.04613750 0.0000000 0.15603333 0.15603333 1.026191e-15 2.639168e-35 FALSE TRUE
ENST00000377674 ENSG00000204859 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB48 protein_coding protein_coding 33.81074 50.65671 20.24123 1.017316 0.7433446 -1.323029 4.976439 7.673168 3.3460572 0.7849959 0.2208173 -1.1949340 0.15431667 0.1521667 0.16590000 0.01373333 8.462163e-01 2.639168e-35 FALSE TRUE
ENST00000435905 ENSG00000204859 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB48 protein_coding protein_coding 33.81074 50.65671 20.24123 1.017316 0.7433446 -1.323029 5.293708 6.138234 2.2604289 1.0705659 1.2028845 -1.4372073 0.16157083 0.1219000 0.10810000 -0.01380000 8.586707e-01 2.639168e-35 FALSE TRUE
ENST00000466813 ENSG00000204859 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB48 protein_coding nonsense_mediated_decay 33.81074 50.65671 20.24123 1.017316 0.7433446 -1.323029 3.486597 9.056226 0.9710830 1.0693586 0.6768889 -3.2080550 0.08289583 0.1783667 0.04663333 -0.13173333 2.691590e-01 2.639168e-35 FALSE TRUE
ENST00000482360 ENSG00000204859 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB48 protein_coding retained_intron 33.81074 50.65671 20.24123 1.017316 0.7433446 -1.323029 5.525807 6.031232 3.7207871 0.4343134 0.3108620 -0.6953627 0.16717917 0.1188333 0.18316667 0.06433333 4.715471e-03 2.639168e-35 FALSE TRUE
MSTRG.206.1 ENSG00000204859 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB48 protein_coding   33.81074 50.65671 20.24123 1.017316 0.7433446 -1.323029 4.017339 5.218610 4.5259348 0.2070802 0.6240094 -0.2050275 0.13299583 0.1029333 0.22483333 0.12190000 4.137561e-03 2.639168e-35 FALSE TRUE
MSTRG.206.3 ENSG00000204859 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB48 protein_coding   33.81074 50.65671 20.24123 1.017316 0.7433446 -1.323029 3.732312 7.100872 0.0000000 0.6815651 0.0000000 -9.4738827 0.09947083 0.1397667 0.00000000 -0.13976667 2.639168e-35 2.639168e-35 FALSE TRUE
MSTRG.206.4 ENSG00000204859 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB48 protein_coding   33.81074 50.65671 20.24123 1.017316 0.7433446 -1.323029 2.278833 5.675287 0.6781649 1.0891192 0.5254437 -3.0464071 0.05772917 0.1118667 0.03546667 -0.07640000 2.912670e-01 2.639168e-35 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000204859 E001 0.7707125 0.0160013003 4.919025e-01 6.291483e-01 1 6579909 6579993 85 + 0.326 0.195 -0.979
ENSG00000204859 E002 0.7707125 0.0160013003 4.919025e-01 6.291483e-01 1 6579994 6579994 1 + 0.326 0.195 -0.979
ENSG00000204859 E003 1.1394067 0.0111304864 3.335012e-01 4.772288e-01 1 6579995 6579996 2 + 0.428 0.245 -1.147
ENSG00000204859 E004 1.2845794 0.0104702567 1.487108e-01 2.599303e-01 1 6579997 6580000 4 + 0.510 0.245 -1.562
ENSG00000204859 E005 1.2845794 0.0104702567 1.487108e-01 2.599303e-01 1 6580001 6580002 2 + 0.510 0.245 -1.562
ENSG00000204859 E006 25.0355471 0.0105423906 6.456677e-03 1.981144e-02 1 6580003 6580016 14 + 1.509 1.260 -0.863
ENSG00000204859 E007 60.0702380 0.0027745100 2.361916e-03 8.343891e-03 1 6580017 6580049 33 + 1.833 1.654 -0.606
ENSG00000204859 E008 60.8093260 0.0006067053 1.562601e-03 5.838166e-03 1 6580050 6580070 21 + 1.833 1.664 -0.573
ENSG00000204859 E009 24.0454102 0.0193908131 6.999608e-01 7.995554e-01 1 6580071 6580087 17 + 1.358 1.309 -0.172
ENSG00000204859 E010 33.1048229 0.0181644185 8.928235e-01 9.354981e-01 1 6580088 6580136 49 + 1.470 1.447 -0.080
ENSG00000204859 E011 483.1227617 0.0050691488 1.325884e-03 5.061962e-03 1 6580541 6581299 759 + 2.695 2.572 -0.410
ENSG00000204859 E012 204.6674880 0.0002021217 9.346417e-04 3.735235e-03 1 6582058 6582299 242 + 2.308 2.205 -0.344
ENSG00000204859 E013 141.2335036 0.0009532290 6.059438e-09 7.791640e-08 1 6585302 6585918 617 + 2.226 2.015 -0.707
ENSG00000204859 E014 170.7869215 0.0002493129 2.283240e-01 3.604303e-01 1 6585919 6586030 112 + 2.190 2.141 -0.163
ENSG00000204859 E015 51.4667852 0.0004645269 1.417476e-06 1.139267e-05 1 6586031 6586206 176 + 1.826 1.564 -0.889
ENSG00000204859 E016 69.9997947 0.0003882741 8.506543e-02 1.675092e-01 1 6586207 6586694 488 + 1.837 1.743 -0.315
ENSG00000204859 E017 167.7565978 0.0002805251 4.488762e-01 5.901005e-01 1 6586695 6586787 93 + 2.135 2.147 0.043
ENSG00000204859 E018 25.5506480 0.0008507548 4.029049e-03 1.322335e-02 1 6586788 6587204 417 + 1.502 1.279 -0.772
ENSG00000204859 E019 150.2907412 0.0002788774 1.162786e-01 2.146516e-01 1 6587205 6587255 51 + 2.065 2.107 0.142
ENSG00000204859 E020 138.3142703 0.0002523977 1.562392e-01 2.701062e-01 1 6587256 6587291 36 + 2.033 2.071 0.130
ENSG00000204859 E021 150.8566295 0.0002833007 1.051163e-02 3.000628e-02 1 6587478 6587514 37 + 2.039 2.116 0.258
ENSG00000204859 E022 242.6414360 0.0002226688 9.848506e-05 5.147946e-04 1 6587515 6587632 118 + 2.227 2.323 0.319
ENSG00000204859 E023 296.6211279 0.0015530605 3.020352e-02 7.236948e-02 1 6588060 6588196 137 + 2.348 2.403 0.181
ENSG00000204859 E024 9.9922976 0.0018434690 9.357947e-01 9.636160e-01 1 6588197 6588277 81 + 0.973 0.952 -0.075
ENSG00000204859 E025 373.2652014 0.0001513444 1.233748e-04 6.292872e-04 1 6588278 6588442 165 + 2.434 2.505 0.237
ENSG00000204859 E026 19.8404719 0.0010346745 1.955395e-01 3.206361e-01 1 6588443 6588686 244 + 1.325 1.203 -0.427
ENSG00000204859 E027 12.0701400 0.0352871815 6.478832e-01 7.594797e-01 1 6588687 6588755 69 + 1.086 1.012 -0.272
ENSG00000204859 E028 290.1466476 0.0002282588 7.558512e-06 5.162554e-05 1 6588756 6588844 89 + 2.297 2.399 0.339
ENSG00000204859 E029 10.5017808 0.0204477406 2.544210e-01 3.911011e-01 1 6588845 6588915 71 + 0.854 1.007 0.575
ENSG00000204859 E030 358.2882973 0.0004605855 3.054369e-08 3.432646e-07 1 6588916 6589337 422 + 2.376 2.497 0.405