ENSG00000204576

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000376557 ENSG00000204576 HEK293_OSMI2_2hA HEK293_TMG_2hB PRR3 protein_coding protein_coding 53.76771 80.67158 36.42663 1.759896 0.3039845 -1.14685 34.900217 58.104996 20.885440 1.7918573 1.2243471 -1.4757223 0.62264167 0.72013333 0.5731000 -0.14703333 0.004562276 1.54395e-13 FALSE TRUE
ENST00000376560 ENSG00000204576 HEK293_OSMI2_2hA HEK293_TMG_2hB PRR3 protein_coding protein_coding 53.76771 80.67158 36.42663 1.759896 0.3039845 -1.14685 11.198087 15.180483 6.561628 1.1341679 0.9362664 -1.2088451 0.20614583 0.18783333 0.1805333 -0.00730000 0.933303273 1.54395e-13 FALSE TRUE
ENST00000498336 ENSG00000204576 HEK293_OSMI2_2hA HEK293_TMG_2hB PRR3 protein_coding processed_transcript 53.76771 80.67158 36.42663 1.759896 0.3039845 -1.14685 3.134527 3.507522 4.127971 0.5385461 0.5369068 0.2343639 0.06672917 0.04356667 0.1132333 0.06966667 0.001759690 1.54395e-13 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000204576 E001 6.929921 0.0026685659 1.429597e-02 3.889936e-02 6 30557280 30557294 15 + 1.027 0.783 -0.926
ENSG00000204576 E002 40.565481 0.0005450413 3.602933e-02 8.364150e-02 6 30557295 30557313 19 + 1.626 1.560 -0.227
ENSG00000204576 E003 43.654567 0.0005223831 2.910027e-01 4.318200e-01 6 30557314 30557315 2 + 1.617 1.612 -0.017
ENSG00000204576 E004 131.761095 0.0003715152 1.717096e-03 6.335793e-03 6 30557316 30557346 31 + 2.115 2.067 -0.163
ENSG00000204576 E005 298.532192 0.0007140527 3.265109e-03 1.104758e-02 6 30557347 30557450 104 + 2.446 2.431 -0.053
ENSG00000204576 E006 91.786611 0.0003231372 9.314539e-07 7.780817e-06 6 30558150 30558212 63 + 2.018 1.886 -0.444
ENSG00000204576 E007 15.187269 0.0011799663 1.661483e-15 6.101239e-14 6 30558213 30558436 224 + 1.508 0.873 -2.272
ENSG00000204576 E008 29.572419 0.0007163510 1.156784e-53 8.765972e-51 6 30560031 30561327 1297 + 1.882 0.886 -3.492
ENSG00000204576 E009 91.654123 0.0170972131 1.861967e-08 2.184038e-07 6 30561328 30561508 181 + 2.144 1.794 -1.174
ENSG00000204576 E010 45.203620 0.0139578288 3.007722e-08 3.384840e-07 6 30561509 30561833 325 + 1.853 1.488 -1.237
ENSG00000204576 E011 604.230912 0.0028904447 1.281891e-01 2.316391e-01 6 30561834 30562124 291 + 2.730 2.745 0.051
ENSG00000204576 E012 2347.758743 0.0014033072 2.255946e-24 2.318949e-22 6 30562389 30563723 1335 + 3.220 3.369 0.496