ENSG00000204498

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000376145 ENSG00000204498 HEK293_OSMI2_2hA HEK293_TMG_2hB NFKBIL1 protein_coding protein_coding 30.14996 58.16204 14.14562 2.62732 0.7260096 -2.03895 5.677092 12.362750 1.665741 1.8130761 0.29175669 -2.884295 0.1691583 0.21180000 0.1166667 -0.09513333 0.06826715 0.0244207 FALSE  
ENST00000376146 ENSG00000204498 HEK293_OSMI2_2hA HEK293_TMG_2hB NFKBIL1 protein_coding protein_coding 30.14996 58.16204 14.14562 2.62732 0.7260096 -2.03895 2.179253 3.481979 1.563216 0.3970636 0.05960042 -1.150329 0.0804250 0.06036667 0.1110667 0.05070000 0.02442070 0.0244207 FALSE  
ENST00000376148 ENSG00000204498 HEK293_OSMI2_2hA HEK293_TMG_2hB NFKBIL1 protein_coding protein_coding 30.14996 58.16204 14.14562 2.62732 0.7260096 -2.03895 21.877096 41.939886 10.675214 2.5875554 0.52527398 -1.973051 0.7364333 0.72143333 0.7550667 0.03363333 0.78330249 0.0244207 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000204498 E001 11.453095 0.0015207420 0.045087536 0.10035833 6 31546870 31546973 104 + 1.143 0.916 -0.834
ENSG00000204498 E002 23.804552 0.0183516553 0.361781237 0.50620302 6 31547608 31547612 5 + 1.324 1.230 -0.330
ENSG00000204498 E003 70.035492 0.0074233576 0.371106858 0.51550873 6 31547613 31547639 27 + 1.636 1.705 0.235
ENSG00000204498 E004 105.306919 0.0080182170 0.288985467 0.42964771 6 31547640 31547660 21 + 1.807 1.882 0.253
ENSG00000204498 E005 186.892196 0.0024765014 0.390974833 0.53492174 6 31547661 31547751 91 + 2.084 2.124 0.133
ENSG00000204498 E006 415.550157 0.0003934858 0.207013067 0.33495614 6 31548163 31548439 277 + 2.486 2.461 -0.084
ENSG00000204498 E007 0.000000       6 31556697 31556783 87 +      
ENSG00000204498 E008 369.389538 0.0001923195 0.619952647 0.73723962 6 31557628 31557804 177 + 2.402 2.417 0.050
ENSG00000204498 E009 167.845918 0.0004407718 0.571033256 0.69741054 6 31557805 31557849 45 + 2.092 2.075 -0.058
ENSG00000204498 E010 2.259234 0.0068521273 0.005091139 0.01617982 6 31557850 31557959 110 + 0.779 0.308 -2.279
ENSG00000204498 E011 888.096571 0.0027068962 0.667140167 0.77446293 6 31558022 31558670 649 + 2.784 2.800 0.052
ENSG00000204498 E012 171.836688 0.0036400096 0.425598528 0.56853407 6 31558671 31558829 159 + 2.109 2.081 -0.093
ENSG00000204498 E013 1.103919 0.0348327201 0.117921542 0.21695525 6 31559845 31559887 43 + 0.498 0.201 -1.868