ENSG00000204228

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374662 ENSG00000204228 HEK293_OSMI2_2hA HEK293_TMG_2hB HSD17B8 protein_coding protein_coding 13.11664 21.79446 15.2462 0.5422416 0.2721103 -0.5152274 10.657197 18.762735 11.562148 0.7090493 0.3425715 -0.6979822 0.7969458 0.8604667 0.7589 -0.1015667 0.03374281 0.03374281 FALSE TRUE
ENST00000469186 ENSG00000204228 HEK293_OSMI2_2hA HEK293_TMG_2hB HSD17B8 protein_coding processed_transcript 13.11664 21.79446 15.2462 0.5422416 0.2721103 -0.5152274 2.459444 3.031725 3.684054 0.2761690 0.4444632 0.2803155 0.2030542 0.1395333 0.2411 0.1015667 0.03380256 0.03374281 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000204228 E001 34.50396 0.0115632979 0.48372041 0.62174452 6 33204655 33204685 31 + 1.478 1.536 0.201
ENSG00000204228 E002 53.56375 0.0080821869 0.86800274 0.91909854 6 33204686 33204720 35 + 1.697 1.710 0.044
ENSG00000204228 E003 24.58412 0.0011129966 0.01044004 0.02983879 6 33204721 33204901 181 + 1.495 1.320 -0.607
ENSG00000204228 E004 145.60424 0.0028510912 0.74138267 0.83058287 6 33204902 33205119 218 + 2.143 2.133 -0.033
ENSG00000204228 E005 150.93144 0.0002393936 0.23135716 0.36410241 6 33205221 33205337 117 + 2.175 2.143 -0.105
ENSG00000204228 E006 141.13670 0.0002579495 0.58161280 0.70613176 6 33205447 33205539 93 + 2.134 2.120 -0.047
ENSG00000204228 E007 147.90841 0.0002542873 0.51000920 0.64526809 6 33205640 33205725 86 + 2.157 2.141 -0.055
ENSG00000204228 E008 118.76510 0.0002986024 0.79495025 0.86902994 6 33205828 33205912 85 + 2.046 2.057 0.037
ENSG00000204228 E009 110.10035 0.0004351007 0.36494645 0.50928518 6 33206134 33206176 43 + 1.997 2.031 0.114
ENSG00000204228 E010 112.29807 0.0033639143 0.36924213 0.51367548 6 33206375 33206449 75 + 2.001 2.044 0.143
ENSG00000204228 E011 85.76989 0.0049777849 0.19298780 0.31752427 6 33206638 33206831 194 + 1.868 1.938 0.236