ENSG00000204227

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374656 ENSG00000204227 HEK293_OSMI2_2hA HEK293_TMG_2hB RING1 protein_coding protein_coding 48.97726 82.39834 35.0498 4.241184 0.7616457 -1.232973 45.75175 80.28592 31.8042 3.767358 0.5537718 -1.335656 0.9175 0.9748 0.9075667 -0.06723333 5.622525e-06 5.622525e-06 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000204227 E001 2.518458 0.0111556863 9.174093e-03 2.671798e-02 6 33208499 33208499 1 + 0.768 0.338 -2.041
ENSG00000204227 E002 7.228872 0.0079117661 3.850771e-03 1.272310e-02 6 33208500 33208508 9 + 1.091 0.727 -1.389
ENSG00000204227 E003 231.151924 0.0046104976 1.729502e-03 6.375733e-03 6 33208509 33208644 136 + 2.384 2.254 -0.433
ENSG00000204227 E004 453.755414 0.0017022972 5.776180e-03 1.802382e-02 6 33208765 33208900 136 + 2.638 2.562 -0.253
ENSG00000204227 E005 4.684470 0.0033810802 1.384883e-06 1.115342e-05 6 33209560 33209625 66 + 1.091 0.442 -2.682
ENSG00000204227 E006 720.288275 0.0004777497 1.426298e-06 1.145871e-05 6 33209626 33209786 161 + 2.845 2.760 -0.283
ENSG00000204227 E007 12.670880 0.0014039665 7.828257e-11 1.406125e-09 6 33209787 33209822 36 + 1.438 0.826 -2.215
ENSG00000204227 E008 909.871120 0.0006753380 5.230258e-03 1.655816e-02 6 33209915 33210130 216 + 2.922 2.872 -0.165
ENSG00000204227 E009 946.745495 0.0006158537 2.678105e-04 1.248607e-03 6 33211158 33211547 390 + 2.861 2.913 0.174
ENSG00000204227 E010 875.409384 0.0001586710 2.448619e-10 4.038687e-09 6 33211729 33212002 274 + 2.802 2.884 0.272
ENSG00000204227 E011 764.453504 0.0005093227 1.066261e-06 8.795629e-06 6 33212298 33212722 425 + 2.747 2.825 0.258