ENSG00000204220

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374606 ENSG00000204220 HEK293_OSMI2_2hA HEK293_TMG_2hB PFDN6 protein_coding protein_coding 140.1374 228.4544 93.55183 9.895108 4.325247 -1.287977 34.97725 53.58958 31.43151 2.006368 1.505230 -0.76955114 0.25392083 0.2362000 0.3382667 0.10206667 7.957186e-02 2.303449e-11 FALSE TRUE
ENST00000374607 ENSG00000204220 HEK293_OSMI2_2hA HEK293_TMG_2hB PFDN6 protein_coding protein_coding 140.1374 228.4544 93.55183 9.895108 4.325247 -1.287977 79.53160 143.48836 39.89997 9.581417 3.902262 -1.84621335 0.54664583 0.6268667 0.4245667 -0.20230000 4.947378e-06 2.303449e-11 FALSE TRUE
ENST00000395131 ENSG00000204220 HEK293_OSMI2_2hA HEK293_TMG_2hB PFDN6 protein_coding protein_coding 140.1374 228.4544 93.55183 9.895108 4.325247 -1.287977 11.03990 11.54220 10.98043 1.229455 1.305894 -0.07191995 0.08770833 0.0505000 0.1165667 0.06606667 1.173617e-05 2.303449e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000204220 E001 6.496449 5.400617e-03 7.285199e-01 8.209800e-01 6 33289193 33289255 63 + 0.862 0.810 -0.199
ENSG00000204220 E002 5.160476 3.314284e-03 9.220139e-01 9.547882e-01 6 33289256 33289280 25 + 0.756 0.737 -0.079
ENSG00000204220 E003 1.109215 7.279779e-02 1.069533e-01 2.010396e-01 6 33289302 33289522 221 + 0.001 0.346 9.833
ENSG00000204220 E004 5.396432 5.818104e-03 4.704714e-01 6.097010e-01 6 33289523 33289595 73 + 0.829 0.721 -0.434
ENSG00000204220 E005 5.026693 1.702132e-02 3.664218e-01 5.107659e-01 6 33289596 33289596 1 + 0.829 0.686 -0.577
ENSG00000204220 E006 6.168290 1.438989e-02 2.532932e-01 3.897976e-01 6 33289597 33289600 4 + 0.920 0.753 -0.650
ENSG00000204220 E007 175.153663 1.183221e-03 3.206122e-25 3.572883e-23 6 33289601 33289620 20 + 2.409 2.076 -1.113
ENSG00000204220 E008 80.015027 5.304526e-03 7.018712e-06 4.833421e-05 6 33289621 33289626 6 + 2.017 1.770 -0.831
ENSG00000204220 E009 99.521823 9.488647e-03 1.174078e-02 3.291635e-02 6 33289627 33289628 2 + 2.051 1.897 -0.516
ENSG00000204220 E010 218.527073 6.514504e-03 3.202077e-05 1.891857e-04 6 33289629 33289769 141 + 2.418 2.215 -0.677
ENSG00000204220 E011 557.586331 7.471253e-04 1.152307e-06 9.444128e-06 6 33289770 33289774 5 + 2.760 2.654 -0.351
ENSG00000204220 E012 943.509616 9.144105e-05 8.194346e-02 1.626089e-01 6 33289775 33289838 64 + 2.934 2.904 -0.099
ENSG00000204220 E013 788.409827 1.228471e-04 1.442185e-01 2.537429e-01 6 33289839 33289841 3 + 2.824 2.838 0.044
ENSG00000204220 E014 1208.893981 2.262817e-04 9.371835e-01 9.645116e-01 6 33289842 33289920 79 + 3.025 3.017 -0.025
ENSG00000204220 E015 1227.645236 1.997624e-04 6.171076e-02 1.295077e-01 6 33290174 33290244 71 + 3.014 3.028 0.045
ENSG00000204220 E016 26.661599 1.660797e-03 8.449240e-02 1.665896e-01 6 33290245 33290325 81 + 1.473 1.340 -0.462
ENSG00000204220 E017 1253.787397 8.646003e-05 8.419948e-13 2.092802e-11 6 33290326 33290450 125 + 2.979 3.052 0.244
ENSG00000204220 E018 103.382831 2.218172e-03 2.902104e-02 7.004828e-02 6 33290451 33290715 265 + 1.882 1.978 0.321
ENSG00000204220 E019 874.784652 9.458739e-05 9.702607e-16 3.669654e-14 6 33290716 33290934 219 + 2.798 2.902 0.347
ENSG00000204220 E020 20.217030 1.948704e-02 8.626849e-03 2.535340e-02 6 33296829 33298401 1573 + 1.450 1.181 -0.942