ENSG00000204186

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374423 ENSG00000204186 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDBF2 protein_coding protein_coding 3.085604 0.7259299 5.598577 0.07870627 0.1948439 2.929994 0.74397475 0.031424217 1.07066456 0.031424217 0.55683474 4.705301 0.16912917 0.041100000 0.19823333 0.1571333 7.190992e-01 9.663377e-13 FALSE TRUE
ENST00000611847 ENSG00000204186 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDBF2 protein_coding protein_coding 3.085604 0.7259299 5.598577 0.07870627 0.1948439 2.929994 0.63291713 0.055878164 1.25789147 0.055878164 0.13355858 4.266487 0.22103750 0.097266667 0.22410000 0.1268333 3.215053e-01 9.663377e-13 FALSE TRUE
ENST00000648361 ENSG00000204186 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDBF2 protein_coding nonsense_mediated_decay 3.085604 0.7259299 5.598577 0.07870627 0.1948439 2.929994 0.22559015 0.000000000 1.04466233 0.000000000 0.20512778 6.720637 0.04038750 0.000000000 0.18610000 0.1861000 6.700105e-04 9.663377e-13 FALSE TRUE
ENST00000649285 ENSG00000204186 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDBF2 protein_coding nonsense_mediated_decay 3.085604 0.7259299 5.598577 0.07870627 0.1948439 2.929994 0.25177667 0.019541131 0.00000000 0.019541131 0.00000000 -1.562725 0.05982917 0.034000000 0.00000000 -0.0340000 2.953418e-01 9.663377e-13 FALSE TRUE
ENST00000649998 ENSG00000204186 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDBF2 protein_coding protein_coding 3.085604 0.7259299 5.598577 0.07870627 0.1948439 2.929994 0.03065239 0.077252217 0.00000000 0.077252217 0.00000000 -3.125192 0.02002083 0.092100000 0.00000000 -0.0921000 3.700037e-01 9.663377e-13 FALSE TRUE
MSTRG.19841.1 ENSG00000204186 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDBF2 protein_coding   3.085604 0.7259299 5.598577 0.07870627 0.1948439 2.929994 0.26029410 0.000000000 0.46751595 0.000000000 0.46751595 5.577477 0.04797500 0.000000000 0.07910000 0.0791000 1.000000e+00 9.663377e-13 FALSE TRUE
MSTRG.19841.4 ENSG00000204186 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDBF2 protein_coding   3.085604 0.7259299 5.598577 0.07870627 0.1948439 2.929994 0.07775649 0.004483764 0.44603442 0.004483764 0.15687970 4.976634 0.01495417 0.005866667 0.08166667 0.0758000 3.426917e-01 9.663377e-13 FALSE TRUE
MSTRG.19841.5 ENSG00000204186 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDBF2 protein_coding   3.085604 0.7259299 5.598577 0.07870627 0.1948439 2.929994 0.22014860 0.508155570 0.01897275 0.116074924 0.01897275 -4.160617 0.29330000 0.681166667 0.00320000 -0.6779667 9.663377e-13 9.663377e-13 FALSE TRUE
MSTRG.19841.8 ENSG00000204186 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDBF2 protein_coding   3.085604 0.7259299 5.598577 0.07870627 0.1948439 2.929994 0.17798400 0.000000000 0.31009903 0.000000000 0.11265862 5.000446 0.03222917 0.000000000 0.05410000 0.0541000 2.298460e-01 9.663377e-13 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000204186 E001 0.1451727 0.0431376053 5.144634e-01   2 206274662 206274662 1 + 0.032 0.000 -7.816
ENSG00000204186 E002 0.1451727 0.0431376053 5.144634e-01   2 206274663 206274665 3 + 0.032 0.000 -8.792
ENSG00000204186 E003 0.2924217 0.0274466377 1.000000e+00   2 206274666 206274683 18 + 0.061 0.000 -9.793
ENSG00000204186 E004 1.0351233 0.0121046960 7.704206e-01 8.517387e-01 2 206274684 206274688 5 + 0.185 0.000 -11.433
ENSG00000204186 E005 1.1823723 0.0112811036 6.271199e-01 7.429507e-01 2 206274689 206274702 14 + 0.206 0.000 -11.593
ENSG00000204186 E006 1.3338878 0.0097675089 5.306272e-01 6.632068e-01 2 206274703 206274704 2 + 0.226 0.000 -11.733
ENSG00000204186 E007 8.7920861 0.0044836248 1.473732e-01 2.581259e-01 2 206274705 206274946 242 + 0.725 0.580 -0.623
ENSG00000204186 E008 0.1451727 0.0431376053 5.144634e-01   2 206274993 206275009 17 + 0.032 0.000 -8.792
ENSG00000204186 E009 0.4375944 0.0261493068 1.000000e+00 1.000000e+00 2 206275010 206275043 34 + 0.089 0.000 -10.336
ENSG00000204186 E010 0.5848434 0.0251092091 1.000000e+00 1.000000e+00 2 206275044 206275079 36 + 0.115 0.000 -10.712
ENSG00000204186 E011 0.5138669 0.0206554017 1.700443e-01 2.882463e-01 2 206275080 206275180 101 + 0.061 0.381 3.213
ENSG00000204186 E012 1.0705091 0.1981175825 1.345381e-01 2.404581e-01 2 206275714 206275991 278 + 0.139 0.577 2.876
ENSG00000204186 E013 6.6582524 0.0376408752 1.200533e-01 2.200608e-01 2 206279540 206279559 20 + 0.636 0.380 -1.253
ENSG00000204186 E014 6.9548268 0.0033549565 8.267108e-02 1.637286e-01 2 206279560 206279592 33 + 0.651 0.380 -1.314
ENSG00000204186 E015 4.1490260 0.0039433380 5.996121e-02 1.265440e-01 2 206280550 206280593 44 + 0.494 0.000 -12.948
ENSG00000204186 E016 1.1909051 0.0107289624 6.277803e-01 7.435052e-01 2 206280594 206280702 109 + 0.206 0.000 -11.594
ENSG00000204186 E017 12.5245085 0.0014270977 2.713939e-02 6.628346e-02 2 206281801 206281909 109 + 0.858 0.580 -1.147
ENSG00000204186 E018 1.6158141 0.0123710606 3.997754e-01 5.435103e-01 2 206292011 206292013 3 + 0.263 0.000 -11.961
ENSG00000204186 E019 6.7815520 0.0046796099 7.397806e-01 8.293982e-01 2 206292014 206292062 49 + 0.621 0.716 0.403
ENSG00000204186 E020 6.6236939 0.0027927469 7.705620e-01 8.518449e-01 2 206292063 206292116 54 + 0.613 0.716 0.438
ENSG00000204186 E021 13.7242811 0.0015966424 6.124575e-01 7.313795e-01 2 206297246 206297332 87 + 0.871 0.973 0.386
ENSG00000204186 E022 12.4720927 0.0014616488 4.907721e-01 6.281138e-01 2 206297333 206297373 41 + 0.839 0.903 0.247
ENSG00000204186 E023 18.0373712 0.0009887343 8.535508e-02 1.679818e-01 2 206304717 206304855 139 + 0.990 0.903 -0.326
ENSG00000204186 E024 124.6106842 0.0882908486 1.092587e-02 3.098309e-02 2 206304856 206306875 2020 + 1.792 1.635 -0.532
ENSG00000204186 E025 16.3472221 0.0010696749 4.282259e-03 1.393369e-02 2 206306876 206307046 171 + 0.962 0.580 -1.545
ENSG00000204186 E026 126.5597033 0.0037051849 5.085925e-09 6.626639e-08 2 206307047 206308755 1709 + 1.801 1.574 -0.770
ENSG00000204186 E027 23.0448836 0.0008089288 1.699759e-01 2.881693e-01 2 206308756 206308917 162 + 1.081 1.086 0.020
ENSG00000204186 E028 121.6869826 0.0003134446 8.378693e-04 3.394389e-03 2 206308918 206310436 1519 + 1.772 1.777 0.018
ENSG00000204186 E029 31.1055426 0.0007445773 1.233009e-01 2.246770e-01 2 206310437 206310782 346 + 1.201 1.215 0.048
ENSG00000204186 E030 228.7022123 0.0375967861 2.740581e-10 4.487810e-09 2 206310783 206314427 3645 + 1.972 2.568 1.989