ENSG00000203993

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371417 ENSG00000203993 HEK293_OSMI2_2hA HEK293_TMG_2hB ARRDC1-AS1 lncRNA lncRNA 25.72768 41.81228 15.52372 2.28326 1.059392 -1.428869 17.033519 32.074157 9.449503 1.4837157 1.00822328 -1.7620248 0.6373875 0.7677667 0.6055000 -0.16226667 0.000123903 0.000123903 FALSE TRUE
ENST00000496793 ENSG00000203993 HEK293_OSMI2_2hA HEK293_TMG_2hB ARRDC1-AS1 lncRNA lncRNA 25.72768 41.81228 15.52372 2.28326 1.059392 -1.428869 4.976477 5.290546 3.459021 0.2006579 0.15852832 -0.6116126 0.2055542 0.1267333 0.2255000 0.09876667 0.001008037 0.000123903 FALSE TRUE
ENST00000663398 ENSG00000203993 HEK293_OSMI2_2hA HEK293_TMG_2hB ARRDC1-AS1 lncRNA lncRNA 25.72768 41.81228 15.52372 2.28326 1.059392 -1.428869 3.379315 3.959707 2.298452 0.3713707 0.05284673 -0.7821068 0.1410083 0.0943000 0.1493333 0.05503333 0.008096412 0.000123903   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000203993 E001 6.021859 0.0133793649 9.797758e-02 0.1874261233 9 137615263 137615325 63 - 0.949 0.725 -0.874
ENSG00000203993 E002 3.602115 0.0043060565 9.940288e-02 0.1895745505 9 137615326 137615331 6 - 0.784 0.537 -1.056
ENSG00000203993 E003 3.716997 0.0059488412 1.331195e-01 0.2384738446 9 137615332 137615333 2 - 0.784 0.559 -0.951
ENSG00000203993 E004 24.246734 0.0009583330 2.723363e-03 0.0094424047 9 137615334 137615366 33 - 1.089 1.372 0.998
ENSG00000203993 E005 628.779144 0.0012960311 2.720566e-05 0.0001634903 9 137615367 137616061 695 - 2.641 2.731 0.301
ENSG00000203993 E006 199.923363 0.0002468899 7.734489e-02 0.1552248107 9 137616062 137616132 71 - 2.167 2.226 0.197
ENSG00000203993 E007 196.520392 0.0003184063 3.030521e-01 0.4449695967 9 137616133 137616199 67 - 2.177 2.215 0.125
ENSG00000203993 E008 111.941570 0.0040760632 3.221418e-03 0.0109192529 9 137616230 137617387 1158 - 2.061 1.935 -0.422
ENSG00000203993 E009 73.940035 0.0004595252 1.117509e-03 0.0043618006 9 137617388 137617933 546 - 1.890 1.749 -0.478
ENSG00000203993 E010 397.758245 0.0002848192 2.251566e-01 0.3566085504 9 137617934 137618191 258 - 2.526 2.509 -0.055
ENSG00000203993 E011 65.908541 0.0004115777 3.928542e-05 0.0002268780 9 137618192 137618530 339 - 1.878 1.691 -0.630
ENSG00000203993 E012 217.150957 0.0002872909 3.740133e-02 0.0862264294 9 137618531 137618906 376 - 2.289 2.239 -0.165