ENSG00000203950

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370775 ENSG00000203950 HEK293_OSMI2_2hA HEK293_TMG_2hB RTL8A protein_coding protein_coding 198.1463 392.8484 106.5481 19.52185 4.059565 -1.882369 60.33360 117.30514 28.550787 5.698337 0.7453294 -2.038282 0.30480417 0.2990000 0.26910000 -0.02990000 0.576425390 0.002610717 FALSE  
ENST00000520964 ENSG00000203950 HEK293_OSMI2_2hA HEK293_TMG_2hB RTL8A protein_coding processed_transcript 198.1463 392.8484 106.5481 19.52185 4.059565 -1.882369 20.04011 45.45732 8.990296 4.807244 1.3672335 -2.336785 0.09387083 0.1166333 0.08366667 -0.03296667 0.353368768 0.002610717 FALSE  
ENST00000522309 ENSG00000203950 HEK293_OSMI2_2hA HEK293_TMG_2hB RTL8A protein_coding processed_transcript 198.1463 392.8484 106.5481 19.52185 4.059565 -1.882369 114.99614 222.70812 68.859964 15.918230 3.1442067 -1.693272 0.58998333 0.5657000 0.64593333 0.08023333 0.002610717 0.002610717 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000203950 E001 22.83471 0.0037647626 1.296812e-05 8.389178e-05 X 135050932 135050939 8 - 0.724 1.277 2.059
ENSG00000203950 E002 991.04728 0.0011066880 1.612034e-04 7.972720e-04 X 135050940 135051057 118 - 2.798 2.853 0.182
ENSG00000203950 E003 3250.02360 0.0011139487 4.588328e-01 5.990992e-01 X 135051058 135051368 311 - 3.373 3.358 -0.049
ENSG00000203950 E004 593.58261 0.0001235901 1.220858e-04 6.233672e-04 X 135051369 135051476 108 - 2.577 2.629 0.171
ENSG00000203950 E005 296.68903 0.0001650333 3.163763e-03 1.074883e-02 X 135051477 135051519 43 - 2.269 2.328 0.196
ENSG00000203950 E006 947.41087 0.0010249986 7.746748e-01 8.547805e-01 X 135051520 135051916 397 - 2.850 2.820 -0.101
ENSG00000203950 E007 1833.40578 0.0024269183 2.142667e-05 1.318956e-04 X 135051917 135052196 280 - 3.204 3.093 -0.368