ENSG00000203666

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366521 ENSG00000203666 HEK293_OSMI2_2hA HEK293_TMG_2hB EFCAB2 protein_coding processed_transcript 4.400425 4.093801 6.520502 0.310342 0.4142131 0.6702331 1.0415004 0.0000000 2.53555223 0.00000000 0.22959590 7.9918349 0.19359583 0.00000000 0.38926667 0.389266667 2.901320e-24 2.90132e-24 FALSE TRUE
ENST00000366522 ENSG00000203666 HEK293_OSMI2_2hA HEK293_TMG_2hB EFCAB2 protein_coding processed_transcript 4.400425 4.093801 6.520502 0.310342 0.4142131 0.6702331 0.2849044 0.4615798 0.38127121 0.02612964 0.05594142 -0.2693329 0.06876667 0.11516667 0.05910000 -0.056066667 1.351585e-01 2.90132e-24 FALSE TRUE
ENST00000366523 ENSG00000203666 HEK293_OSMI2_2hA HEK293_TMG_2hB EFCAB2 protein_coding protein_coding 4.400425 4.093801 6.520502 0.310342 0.4142131 0.6702331 0.5446553 0.7016037 0.86817480 0.16680002 0.23945897 0.3034341 0.13397917 0.16700000 0.13836667 -0.028633333 8.181552e-01 2.90132e-24 FALSE TRUE
ENST00000425550 ENSG00000203666 HEK293_OSMI2_2hA HEK293_TMG_2hB EFCAB2 protein_coding protein_coding 4.400425 4.093801 6.520502 0.310342 0.4142131 0.6702331 0.2513121 0.2281131 0.25350940 0.05368027 0.25350940 0.1462074 0.05556667 0.05853333 0.03673333 -0.021800000 3.998000e-01 2.90132e-24 FALSE FALSE
ENST00000427529 ENSG00000203666 HEK293_OSMI2_2hA HEK293_TMG_2hB EFCAB2 protein_coding protein_coding 4.400425 4.093801 6.520502 0.310342 0.4142131 0.6702331 0.3393740 0.1871515 0.25536172 0.18715155 0.25536172 0.4286552 0.08714167 0.04310000 0.03666667 -0.006433333 9.741624e-01 2.90132e-24   FALSE
ENST00000497591 ENSG00000203666 HEK293_OSMI2_2hA HEK293_TMG_2hB EFCAB2 protein_coding processed_transcript 4.400425 4.093801 6.520502 0.310342 0.4142131 0.6702331 0.6490486 0.6070890 0.93412438 0.13680673 0.09300302 0.6134985 0.15103333 0.14480000 0.14350000 -0.001300000 9.803477e-01 2.90132e-24   FALSE
ENST00000518607 ENSG00000203666 HEK293_OSMI2_2hA HEK293_TMG_2hB EFCAB2 protein_coding retained_intron 4.400425 4.093801 6.520502 0.310342 0.4142131 0.6702331 0.2519542 0.5406584 0.05354528 0.01373957 0.05354528 -3.1153009 0.06370000 0.13366667 0.00940000 -0.124266667 5.981656e-03 2.90132e-24   FALSE
ENST00000549220 ENSG00000203666 HEK293_OSMI2_2hA HEK293_TMG_2hB EFCAB2 protein_coding processed_transcript 4.400425 4.093801 6.520502 0.310342 0.4142131 0.6702331 0.3382571 0.2990704 0.79863273 0.15036272 0.30399275 1.3875491 0.07697500 0.07693333 0.12320000 0.046266667 7.577565e-01 2.90132e-24 FALSE TRUE
MSTRG.3403.4 ENSG00000203666 HEK293_OSMI2_2hA HEK293_TMG_2hB EFCAB2 protein_coding   4.400425 4.093801 6.520502 0.310342 0.4142131 0.6702331 0.3443481 0.9819233 0.27535890 0.15963234 0.27535890 -1.7974509 0.08745417 0.24133333 0.03990000 -0.201433333 8.063494e-02 2.90132e-24 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000203666 E001 0.2214452 0.0400602287 3.721714e-01   1 244969682 244969704 23 + 0.000 0.138 10.436
ENSG00000203666 E002 0.2214452 0.0400602287 3.721714e-01   1 244969705 244969738 34 + 0.000 0.138 11.902
ENSG00000203666 E003 0.3686942 0.0261539511 8.396036e-01 8.998054e-01 1 244969739 244969798 60 + 0.108 0.138 0.403
ENSG00000203666 E004 4.3186875 0.0043673843 2.426922e-03 8.542952e-03 1 244969799 244969981 183 + 0.475 0.908 1.840
ENSG00000203666 E005 6.9821424 0.0027642901 4.461647e-03 1.443374e-02 1 244969982 244970043 62 + 0.720 1.059 1.299
ENSG00000203666 E006 3.7695245 0.0043983289 3.951375e-01 5.389697e-01 1 244970044 244970285 242 + 0.614 0.739 0.526
ENSG00000203666 E007 3.2053077 0.0051199808 2.993096e-01 4.408215e-01 1 244970286 244970326 41 + 0.550 0.708 0.689
ENSG00000203666 E008 2.7645928 0.0055779627 2.033282e-01 3.303652e-01 1 244970327 244970328 2 + 0.475 0.675 0.914
ENSG00000203666 E009 10.2147090 0.0017653155 5.173341e-02 1.122432e-01 1 244970329 244970350 22 + 0.951 1.149 0.722
ENSG00000203666 E010 9.7364182 0.0018161334 1.193241e-01 2.189742e-01 1 244970351 244970355 5 + 0.951 1.113 0.593
ENSG00000203666 E011 15.7379548 0.0012031437 4.024106e-02 9.147636e-02 1 244970356 244970389 34 + 1.129 1.303 0.614
ENSG00000203666 E012 19.9087256 0.0011394350 5.713074e-01 6.976206e-01 1 244970390 244970404 15 + 1.336 1.294 -0.145
ENSG00000203666 E013 23.2698282 0.0009006728 8.411282e-01 9.008969e-01 1 244970405 244970422 18 + 1.389 1.377 -0.043
ENSG00000203666 E014 27.3632994 0.0152896450 6.602858e-01 7.689750e-01 1 244970423 244970443 21 + 1.467 1.426 -0.144
ENSG00000203666 E015 0.4428904 0.5491714939 2.443157e-01 3.792754e-01 1 244970444 244970496 53 + 0.000 0.254 12.731
ENSG00000203666 E016 13.8167752 0.0013020564 2.910395e-02 7.020545e-02 1 245002121 245002152 32 + 1.255 1.059 -0.699
ENSG00000203666 E017 20.7656289 0.0050250277 3.619592e-03 1.206639e-02 1 245002153 245002232 80 + 1.433 1.193 -0.837
ENSG00000203666 E018 32.2543910 0.0006361623 1.286890e-03 4.933115e-03 1 245017242 245017326 85 + 1.603 1.410 -0.664
ENSG00000203666 E019 0.0000000       1 245030655 245030831 177 +      
ENSG00000203666 E020 37.9489510 0.0006449335 8.373380e-03 2.472123e-02 1 245059386 245059458 73 + 1.655 1.511 -0.493
ENSG00000203666 E021 0.7500885 0.0709406361 3.023507e-01 4.442160e-01 1 245075574 245075611 38 + 0.329 0.137 -1.607
ENSG00000203666 E022 43.6138758 0.0006363068 5.400656e-01 6.712623e-01 1 245082083 245082162 80 + 1.668 1.639 -0.098
ENSG00000203666 E023 28.8765177 0.0013250157 4.871921e-01 6.248739e-01 1 245083442 245083455 14 + 1.503 1.462 -0.141
ENSG00000203666 E024 27.7716536 0.0007831033 4.311831e-01 5.737609e-01 1 245083456 245083484 29 + 1.488 1.440 -0.165
ENSG00000203666 E025 33.8189699 0.0006558265 6.397994e-01 7.531305e-01 1 245083622 245083688 67 + 1.533 1.562 0.099
ENSG00000203666 E026 2.6561447 0.0058333053 9.455013e-02 1.822146e-01 1 245083689 245083860 172 + 0.670 0.397 -1.299
ENSG00000203666 E027 1.4865465 0.0561222571 2.315286e-01 3.642874e-01 1 245086733 245086759 27 + 0.475 0.244 -1.397
ENSG00000203666 E028 3.2506643 0.0128050987 2.491906e-01 3.849568e-01 1 245086760 245086961 202 + 0.696 0.512 -0.818
ENSG00000203666 E029 30.3076160 0.0071165689 5.795651e-01 7.044569e-01 1 245087245 245087837 593 + 1.515 1.478 -0.128
ENSG00000203666 E030 11.0504990 0.0342103232 8.999875e-01 9.401965e-01 1 245091027 245091493 467 + 1.070 1.107 0.131
ENSG00000203666 E031 5.5785934 0.1134374440 6.105222e-01 7.298459e-01 1 245091494 245091806 313 + 0.764 0.880 0.453
ENSG00000203666 E032 0.1472490 0.0433590926 6.338294e-01   1 245107179 245107297 119 + 0.108 0.000 -11.464
ENSG00000203666 E033 60.8489304 0.0012488415 4.207983e-07 3.779046e-06 1 245121920 245127164 5245 + 1.668 1.894 0.763