ENSG00000198464

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000468240 ENSG00000198464 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF480 protein_coding nonsense_mediated_decay 4.872962 3.082034 6.828844 0.277357 0.3323627 1.145196 1.3201660 1.8408025 1.5442366 0.35354786 0.35626126 -0.2519449 0.33438333 0.5906333 0.2221000 -0.3685333 0.002041742 0.002041742 TRUE TRUE
ENST00000595962 ENSG00000198464 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF480 protein_coding protein_coding 4.872962 3.082034 6.828844 0.277357 0.3323627 1.145196 3.0188561 0.8927891 4.5888585 0.22098647 0.08588876 2.3488149 0.55836667 0.2951000 0.6762333 0.3811333 0.003502487 0.002041742 FALSE TRUE
MSTRG.17619.1 ENSG00000198464 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF480 protein_coding   4.872962 3.082034 6.828844 0.277357 0.3323627 1.145196 0.4505577 0.2647143 0.5898377 0.02541572 0.08187744 1.1266401 0.08798333 0.0880000 0.0864000 -0.0016000 1.000000000 0.002041742 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000198464 E001 0.5483223 0.0236732154 3.197038e-01 4.627916e-01 19 52297164 52297168 5 + 0.106 0.248 1.485
ENSG00000198464 E002 2.5640637 0.0593557005 6.792598e-01 7.834960e-01 19 52297169 52297176 8 + 0.605 0.468 -0.644
ENSG00000198464 E003 3.4487132 0.0628412175 6.343587e-01 7.488338e-01 19 52297177 52297178 2 + 0.633 0.652 0.081
ENSG00000198464 E004 65.8500985 0.0004377843 1.666672e-09 2.369644e-08 19 52297179 52297223 45 + 1.731 1.918 0.631
ENSG00000198464 E005 102.5660368 0.0004089476 2.302618e-21 1.721484e-19 19 52300394 52300484 91 + 1.886 2.136 0.836
ENSG00000198464 E006 113.9024996 0.0004213080 1.909146e-21 1.439817e-19 19 52314153 52314279 127 + 1.942 2.174 0.778
ENSG00000198464 E007 96.7816442 0.0009976569 3.946909e-17 1.781158e-15 19 52315834 52315962 129 + 1.869 2.106 0.795
ENSG00000198464 E008 0.4782907 0.0213700716 3.247744e-02 7.676453e-02 19 52317416 52317513 98 + 0.000 0.333 11.218
ENSG00000198464 E009 184.7728087 0.0002489459 4.409358e-43 1.831885e-40 19 52321579 52323674 2096 + 2.415 1.996 -1.400
ENSG00000198464 E010 172.4165845 0.0175538664 5.118705e-10 7.995081e-09 19 52323675 52325882 2208 + 2.388 1.952 -1.459
ENSG00000198464 E011 3.5137388 0.0048484271 8.692994e-01 9.200053e-01 19 52325883 52325922 40 + 0.686 0.610 -0.325