ENSG00000198315

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000330236 ENSG00000198315 HEK293_OSMI2_2hA HEK293_TMG_2hB ZKSCAN8 protein_coding protein_coding 12.54711 4.829905 18.97665 0.3431832 0.4477024 1.971935 11.2775745 4.730322 16.733151 0.2951907 0.2036367 1.820514 0.89267083 0.981 0.88226667 -0.09873333 0.0828455335 0.0003403074 FALSE  
ENST00000606198 ENSG00000198315 HEK293_OSMI2_2hA HEK293_TMG_2hB ZKSCAN8 protein_coding nonsense_mediated_decay 12.54711 4.829905 18.97665 0.3431832 0.4477024 1.971935 0.8149219 0.000000 1.192057 0.0000000 0.4442884 6.909361 0.07255417 0.000 0.06193333 0.06193333 0.0003403074 0.0003403074 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000198315 E001 10.887097 0.0017000399 3.792754e-06 2.774660e-05 6 28141883 28141909 27 + 0.941 0.470 -1.987
ENSG00000198315 E002 35.334222 0.0051802587 7.972912e-08 8.255599e-07 6 28141910 28141937 28 + 1.393 1.245 -0.516
ENSG00000198315 E003 53.755977 0.0005462891 2.690303e-12 6.159152e-11 6 28141938 28142029 92 + 1.567 1.436 -0.448
ENSG00000198315 E004 0.599719 0.1301236772 4.271012e-01 5.699393e-01 6 28142211 28142263 53 + 0.157 0.001 -8.238
ENSG00000198315 E005 5.153480 0.0172553162 2.199012e-03 7.839007e-03 6 28144331 28144424 94 + 0.672 0.295 -1.925
ENSG00000198315 E006 166.723889 0.0002756686 1.569392e-34 3.730447e-32 6 28148316 28148824 509 + 2.050 1.928 -0.408
ENSG00000198315 E007 3.589667 0.1131720919 7.623224e-03 2.283279e-02 6 28148825 28148838 14 + 0.559 0.001 -10.828
ENSG00000198315 E008 89.587109 0.0030505984 3.662334e-14 1.108867e-12 6 28149483 28149624 142 + 1.780 1.688 -0.313
ENSG00000198315 E009 74.949313 0.0003886239 7.756757e-19 4.343761e-17 6 28151845 28151936 92 + 1.713 1.533 -0.614
ENSG00000198315 E010 75.927205 0.0003765138 4.502761e-16 1.779380e-14 6 28152261 28152384 124 + 1.713 1.602 -0.379
ENSG00000198315 E011 1901.643708 0.0115026288 1.297874e-23 1.231239e-21 6 28153056 28159460 6405 + 3.001 3.469 1.558