ENSG00000197977

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000354666 ENSG00000197977 HEK293_OSMI2_2hA HEK293_TMG_2hB ELOVL2 protein_coding protein_coding 8.701383 6.996924 13.65876 0.05422732 0.4312886 0.964029 6.2832139 3.4231565 10.8992587 0.04405205 0.3866799 1.667946 0.6753917 0.48923333 0.79783333 0.3086000 4.195298e-18 1.207076e-19 FALSE TRUE
MSTRG.27648.2 ENSG00000197977 HEK293_OSMI2_2hA HEK293_TMG_2hB ELOVL2 protein_coding   8.701383 6.996924 13.65876 0.05422732 0.4312886 0.964029 0.4471402 0.1023461 0.9699278 0.05283270 0.1987775 3.124725 0.0458625 0.01463333 0.07123333 0.0566000 2.360448e-02 1.207076e-19 FALSE TRUE
MSTRG.27648.3 ENSG00000197977 HEK293_OSMI2_2hA HEK293_TMG_2hB ELOVL2 protein_coding   8.701383 6.996924 13.65876 0.05422732 0.4312886 0.964029 1.9097661 3.4714209 1.5893350 0.05123409 0.1919831 -1.122204 0.2722458 0.49616667 0.11620000 -0.3799667 1.207076e-19 1.207076e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000197977 E001 503.7970086 0.0002487348 5.261905e-02 1.138159e-01 6 10980759 10983654 2896 - 2.666 2.693 0.090
ENSG00000197977 E002 73.7279072 0.0005178660 4.124015e-09 5.453597e-08 6 10983655 10983906 252 - 1.918 1.638 -0.946
ENSG00000197977 E003 59.6434646 0.0032874714 4.233743e-04 1.864027e-03 6 10989703 10989837 135 - 1.809 1.607 -0.685
ENSG00000197977 E004 59.4925010 0.0011584326 5.011612e-03 1.596279e-02 6 10990318 10990442 125 - 1.793 1.649 -0.490
ENSG00000197977 E005 78.3843216 0.0003963303 3.420838e-07 3.128343e-06 6 10995007 10995178 172 - 1.934 1.705 -0.772
ENSG00000197977 E006 1.2824144 0.0152311763 7.549162e-03 2.264247e-02 6 10999742 10999769 28 - 0.149 0.613 2.922
ENSG00000197977 E007 18.1058271 0.0010986004 1.081471e-23 1.035278e-21 6 10999770 10999918 149 - 0.801 1.633 2.979
ENSG00000197977 E008 81.5888762 0.0004041839 2.116332e-03 7.585113e-03 6 11000087 11000164 78 - 1.847 1.971 0.418
ENSG00000197977 E009 101.5577536 0.0002990412 9.803779e-02 1.875280e-01 6 11005372 11005559 188 - 1.960 2.022 0.207
ENSG00000197977 E010 42.0368706 0.0007183291 1.659713e-01 2.829495e-01 6 11010746 11010809 64 - 1.573 1.654 0.275
ENSG00000197977 E011 0.5202097 0.0209270145 8.454629e-01 9.039124e-01 6 11012482 11012518 37 - 0.149 0.181 0.342
ENSG00000197977 E012 29.6978482 0.0115171911 1.845045e-01 3.068601e-01 6 11044228 11044375 148 - 1.414 1.524 0.376