ENSG00000197965

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000359523 ENSG00000197965 HEK293_OSMI2_2hA HEK293_TMG_2hB MPZL1 protein_coding protein_coding 46.8619 36.65937 56.11288 2.771894 1.308582 0.6140136 19.601634 20.917456 18.66530 2.4536848 0.3139956 -0.1642655 0.43926250 0.5683000 0.3329000 -0.2354000 1.819389e-06 3.236376e-21 FALSE TRUE
ENST00000367853 ENSG00000197965 HEK293_OSMI2_2hA HEK293_TMG_2hB MPZL1 protein_coding protein_coding 46.8619 36.65937 56.11288 2.771894 1.308582 0.6140136 13.462802 10.058635 13.79048 0.8459311 1.5045720 0.4548508 0.28636667 0.2741333 0.2453333 -0.0288000 6.837365e-01 3.236376e-21 FALSE FALSE
ENST00000448405 ENSG00000197965 HEK293_OSMI2_2hA HEK293_TMG_2hB MPZL1 protein_coding nonsense_mediated_decay 46.8619 36.65937 56.11288 2.771894 1.308582 0.6140136 5.998265 1.505411 11.06326 1.4114377 0.3631194 2.8692994 0.11497500 0.0400000 0.1972333 0.1572333 9.025183e-02 3.236376e-21 TRUE FALSE
ENST00000474859 ENSG00000197965 HEK293_OSMI2_2hA HEK293_TMG_2hB MPZL1 protein_coding protein_coding 46.8619 36.65937 56.11288 2.771894 1.308582 0.6140136 3.398617 0.000000 7.84017 0.0000000 1.4810290 9.6165801 0.05822917 0.0000000 0.1398667 0.1398667 3.236376e-21 3.236376e-21 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000197965 E001 0.1515154 0.0432860400 5.397724e-01   1 167721192 167721328 137 + 0.112 0.000 -8.447
ENSG00000197965 E002 0.6621732 0.1286917181 9.352956e-01 9.632556e-01 1 167721619 167721750 132 + 0.201 0.236 0.294
ENSG00000197965 E003 0.7061804 0.1470882462 8.914078e-01 9.346403e-01 1 167721864 167721949 86 + 0.201 0.238 0.304
ENSG00000197965 E004 6.7644162 0.0262724800 1.169605e-01 2.156205e-01 1 167721950 167721981 32 + 0.966 0.783 -0.704
ENSG00000197965 E005 7.2782832 0.0184897139 9.114540e-02 1.769219e-01 1 167721982 167721983 2 + 0.993 0.808 -0.704
ENSG00000197965 E006 10.4768008 0.0259321160 2.465926e-02 6.117814e-02 1 167721984 167721988 5 + 1.163 0.915 -0.910
ENSG00000197965 E007 16.4407670 0.0023501728 6.461945e-04 2.702419e-03 1 167721989 167721992 4 + 1.346 1.098 -0.879
ENSG00000197965 E008 147.4967642 0.0315102592 4.012454e-03 1.317701e-02 1 167721993 167722151 159 + 2.267 2.045 -0.742
ENSG00000197965 E009 139.3896680 0.0248885487 2.748306e-03 9.516770e-03 1 167722152 167722242 91 + 2.238 2.029 -0.700
ENSG00000197965 E010 0.0000000       1 167765058 167765569 512 +      
ENSG00000197965 E011 0.0000000       1 167765570 167765582 13 +      
ENSG00000197965 E012 267.1135331 0.0040368753 2.452505e-11 4.795826e-10 1 167765583 167765749 167 + 2.520 2.313 -0.690
ENSG00000197965 E013 3.8214794 0.0403365667 9.470826e-04 3.778577e-03 1 167765750 167765896 147 + 0.275 0.877 2.890
ENSG00000197965 E014 380.4194605 0.0001499841 1.027423e-29 1.689603e-27 1 167772275 167772488 214 + 2.656 2.500 -0.521
ENSG00000197965 E015 313.3077434 0.0001670554 7.087858e-18 3.538270e-16 1 167773236 167773368 133 + 2.558 2.436 -0.409
ENSG00000197965 E016 3.5752390 0.0042912211 3.433062e-02 8.039676e-02 1 167773369 167773909 541 + 0.778 0.500 -1.212
ENSG00000197965 E017 3.0924809 0.1738453768 4.466026e-02 9.957808e-02 1 167774556 167774847 292 + 0.777 0.379 -1.841
ENSG00000197965 E018 238.1307400 0.0002286937 4.100020e-13 1.069001e-11 1 167776064 167776166 103 + 2.437 2.319 -0.395
ENSG00000197965 E019 1.5533704 0.2103478241 5.573029e-02 1.192340e-01 1 167787067 167787265 199 + 0.562 0.135 -2.859
ENSG00000197965 E020 537.9482861 0.0034295147 6.630629e-07 5.723574e-06 1 167787820 167788137 318 + 2.783 2.675 -0.360
ENSG00000197965 E021 2501.2926734 0.0003410316 2.616945e-18 1.369159e-16 1 167788138 167790126 1989 + 3.343 3.454 0.368
ENSG00000197965 E022 975.7492556 0.0069316754 1.713046e-12 4.037968e-11 1 167790127 167791919 1793 + 2.811 3.119 1.024