ENSG00000197857

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355684 ENSG00000197857 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF44 protein_coding protein_coding 2.152723 1.338066 2.912912 0.1244481 0.1816146 1.116515 1.52612073 0.54224491 2.2265880 0.13097910 0.05871778 2.017919405 0.68837083 0.394666667 0.7682333 0.37356667 0.0004553951 0.0004553951 FALSE TRUE
ENST00000356109 ENSG00000197857 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF44 protein_coding protein_coding 2.152723 1.338066 2.912912 0.1244481 0.1816146 1.116515 0.05554963 0.28764643 0.0000000 0.16391326 0.00000000 -4.895527669 0.04854583 0.239366667 0.0000000 -0.23936667 0.1064123674 0.0004553951 FALSE TRUE
ENST00000393337 ENSG00000197857 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF44 protein_coding nonsense_mediated_decay 2.152723 1.338066 2.912912 0.1244481 0.1816146 1.116515 0.14067141 0.00911924 0.1840119 0.00911924 0.02525837 3.343048326 0.05323750 0.007766667 0.0642000 0.05643333 0.0701216320 0.0004553951 TRUE TRUE
ENST00000483826 ENSG00000197857 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF44 protein_coding retained_intron 2.152723 1.338066 2.912912 0.1244481 0.1816146 1.116515 0.38160490 0.45604804 0.4590698 0.12841362 0.16105407 0.009324054 0.18772917 0.330200000 0.1523667 -0.17783333 0.1942985175 0.0004553951   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000197857 E001 0.2998086 0.029957661 0.514457787   19 12224686 12225279 594 - 0.082 0.179 1.302
ENSG00000197857 E002 0.1515154 0.042536151 1.000000000   19 12226159 12226371 213 - 0.082 0.000 -10.196
ENSG00000197857 E003 0.2998086 0.029957661 0.514457787   19 12226372 12226527 156 - 0.082 0.179 1.302
ENSG00000197857 E004 0.1515154 0.042536151 1.000000000   19 12230460 12230515 56 - 0.082 0.000 -10.196
ENSG00000197857 E005 0.0000000       19 12234667 12234908 242 -      
ENSG00000197857 E006 0.0000000       19 12237452 12237556 105 -      
ENSG00000197857 E007 0.0000000       19 12237557 12237589 33 -      
ENSG00000197857 E008 0.2903454 0.324069634 0.671342672   19 12237899 12238102 204 - 0.150 0.000 -10.703
ENSG00000197857 E009 0.1451727 0.042878157 1.000000000   19 12242707 12242844 138 - 0.082 0.000 -10.195
ENSG00000197857 E010 0.8062886 0.017189718 0.653503506 0.763952026 19 12244581 12244673 93 - 0.262 0.180 -0.693
ENSG00000197857 E011 2.4453973 0.006477281 0.022194778 0.056075085 19 12247643 12248402 760 - 0.613 0.179 -2.601
ENSG00000197857 E012 1.0695787 0.011759416 0.362967858 0.507391157 19 12248403 12248678 276 - 0.351 0.179 -1.280
ENSG00000197857 E013 0.9244060 0.013545719 0.487940581 0.625576108 19 12249978 12250038 61 - 0.309 0.179 -1.017
ENSG00000197857 E014 1.0414660 0.012330440 0.056252005 0.120152137 19 12250242 12250368 127 - 0.389 0.000 -12.586
ENSG00000197857 E015 21.0594528 0.037623893 0.301006901 0.442780805 19 12271808 12272486 679 - 1.278 1.400 0.425
ENSG00000197857 E016 74.4552698 0.000762433 0.925557384 0.957128734 19 12272487 12274063 1577 - 1.847 1.849 0.006
ENSG00000197857 E017 5.1390782 0.005208517 0.184292698 0.306615609 19 12274973 12274978 6 - 0.805 0.610 -0.807
ENSG00000197857 E018 7.3588751 0.003202270 0.139678263 0.247573332 19 12274979 12275033 55 - 0.938 0.749 -0.732
ENSG00000197857 E019 11.7350050 0.001703013 0.121207159 0.221658171 19 12275956 12276082 127 - 1.121 0.963 -0.577
ENSG00000197857 E020 2.2789282 0.016976417 0.280459149 0.420312666 19 12291028 12291219 192 - 0.424 0.610 0.892
ENSG00000197857 E021 2.2777823 0.096606623 0.150253768 0.262025365 19 12291220 12291250 31 - 0.389 0.661 1.306
ENSG00000197857 E022 4.7970680 0.004180609 0.000232166 0.001100624 19 12293232 12293375 144 - 0.515 1.009 2.015
ENSG00000197857 E023 12.1894866 0.002014783 0.866404965 0.917988886 19 12294692 12294883 192 - 1.100 1.088 -0.043