ENSG00000197728

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000548590 ENSG00000197728 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS26 protein_coding retained_intron 228.8899 465.8733 116.0768 16.53511 7.240401 -2.004765 6.172281 3.154995 10.0937800 0.3032274 0.1476059 1.6746201 0.05309583 0.006766667 0.0875 0.08073333 2.168629e-60 2.168629e-60   FALSE
ENST00000552361 ENSG00000197728 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS26 protein_coding protein_coding 228.8899 465.8733 116.0768 16.53511 7.240401 -2.004765 9.486120 29.255506 0.3985007 7.7877380 0.3985007 -6.1627190 0.03229583 0.063666667 0.0038 -0.05986667 3.202299e-02 2.168629e-60 FALSE FALSE
ENST00000646449 ENSG00000197728 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS26 protein_coding protein_coding 228.8899 465.8733 116.0768 16.53511 7.240401 -2.004765 197.345597 410.045263 89.7356889 20.1123370 4.3170897 -2.1919038 0.82686667 0.879366667 0.7745 -0.10486667 2.224130e-03 2.168629e-60 FALSE FALSE
MSTRG.7442.7 ENSG00000197728 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS26 protein_coding   228.8899 465.8733 116.0768 16.53511 7.240401 -2.004765 9.543897 8.600968 10.8302800 1.8435035 2.3944282 0.3321547 0.05311667 0.018233333 0.0917 0.07346667 3.031986e-05 2.168629e-60 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000197728 E001 0.000000       12 56041351 56041396 46 +      
ENSG00000197728 E002 1.921671 0.0512792698 9.004629e-01 9.404995e-01 12 56041744 56041810 67 + 0.392 0.384 -0.045
ENSG00000197728 E003 21.064626 0.0011916027 1.683279e-01 2.860198e-01 12 56041811 56041892 82 + 1.287 1.172 -0.405
ENSG00000197728 E004 48.510975 0.0111852563 3.387376e-01 4.826259e-01 12 56041893 56041916 24 + 1.593 1.526 -0.226
ENSG00000197728 E005 50.019127 0.0058425315 2.489179e-01 3.846610e-01 12 56041917 56041917 1 + 1.609 1.538 -0.242
ENSG00000197728 E006 603.535901 0.0005724758 3.829553e-01 5.271016e-01 12 56041918 56042064 147 + 2.624 2.622 -0.007
ENSG00000197728 E007 612.984178 0.0003099319 1.957249e-01 3.208679e-01 12 56042065 56042132 68 + 2.638 2.628 -0.031
ENSG00000197728 E008 436.091916 0.0011437994 7.165513e-01 8.121795e-01 12 56042133 56042139 7 + 2.476 2.483 0.024
ENSG00000197728 E009 4939.707600 0.0020041217 1.049988e-02 2.997815e-02 12 56042140 56042169 30 + 3.475 3.544 0.229
ENSG00000197728 E010 3.151389 0.0324737432 1.758407e-07 1.703877e-06 12 56042170 56042186 17 + 1.080 0.269 -3.682
ENSG00000197728 E011 28.097516 0.0032740071 1.941486e-12 4.538606e-11 12 56042231 56042346 116 + 1.707 1.203 -1.738
ENSG00000197728 E012 9886.053013 0.0002144815 4.699514e-06 3.365778e-05 12 56042425 56042602 178 + 3.795 3.843 0.160
ENSG00000197728 E013 107.712022 0.0004544221 8.183017e-228 5.167493e-223 12 56042603 56043049 447 + 2.572 1.449 -3.779
ENSG00000197728 E014 87.951706 0.0075210764 5.691416e-73 9.295015e-70 12 56043050 56043362 313 + 2.444 1.438 -3.388
ENSG00000197728 E015 9827.091871 0.0006861435 5.153649e-01 6.500462e-01 12 56043363 56043493 131 + 3.824 3.835 0.037
ENSG00000197728 E016 5391.670894 0.0009183454 8.220172e-01 8.877780e-01 12 56044119 56044697 579 + 3.561 3.575 0.049