ENSG00000197223

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355848 ENSG00000197223 HEK293_OSMI2_2hA HEK293_TMG_2hB C1D protein_coding protein_coding 18.84744 8.818631 31.05238 2.12926 1.26766 1.814907 0.9465526 0.3981195 1.722818 0.1672635 0.1316242 2.086057 0.0540500 0.04890000 0.0555000 0.0066000 8.181801e-01 1.279824e-16 FALSE FALSE
ENST00000407324 ENSG00000197223 HEK293_OSMI2_2hA HEK293_TMG_2hB C1D protein_coding protein_coding 18.84744 8.818631 31.05238 2.12926 1.26766 1.814907 1.9555105 5.0771731 2.176855 1.7155446 0.3046281 -1.218007 0.2129292 0.55396667 0.0697000 -0.4842667 1.279824e-16 1.279824e-16 FALSE TRUE
ENST00000410067 ENSG00000197223 HEK293_OSMI2_2hA HEK293_TMG_2hB C1D protein_coding protein_coding 18.84744 8.818631 31.05238 2.12926 1.26766 1.814907 3.5022822 1.1386328 6.021258 0.1571790 0.2907967 2.392541 0.1785667 0.13496667 0.1939667 0.0590000 4.992579e-02 1.279824e-16 FALSE FALSE
ENST00000485709 ENSG00000197223 HEK293_OSMI2_2hA HEK293_TMG_2hB C1D protein_coding retained_intron 18.84744 8.818631 31.05238 2.12926 1.26766 1.814907 2.3235036 0.8356997 3.686670 0.1045312 0.1789329 2.128009 0.1091792 0.10193333 0.1194333 0.0175000 7.249820e-01 1.279824e-16 TRUE TRUE
MSTRG.18549.5 ENSG00000197223 HEK293_OSMI2_2hA HEK293_TMG_2hB C1D protein_coding   18.84744 8.818631 31.05238 2.12926 1.26766 1.814907 5.7804052 0.6626329 10.721428 0.1665808 0.4364031 3.995879 0.2572375 0.07703333 0.3474667 0.2704333 1.441976e-09 1.279824e-16 FALSE TRUE
MSTRG.18549.6 ENSG00000197223 HEK293_OSMI2_2hA HEK293_TMG_2hB C1D protein_coding   18.84744 8.818631 31.05238 2.12926 1.26766 1.814907 3.8864898 0.6702928 6.109537 0.1455497 1.1520428 3.169195 0.1578208 0.07810000 0.1944000 0.1163000 1.209150e-02 1.279824e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000197223 E001 5.9256363 0.0102294080 5.760303e-01 7.015346e-01 2 68041130 68042208 1079 - 0.769 0.872 0.407
ENSG00000197223 E002 132.0808085 0.0052502399 1.061556e-29 1.742430e-27 2 68042209 68042676 468 - 1.864 2.395 1.778
ENSG00000197223 E003 34.1015907 0.0006901346 2.517071e-28 3.665269e-26 2 68042677 68042713 37 - 1.193 1.881 2.363
ENSG00000197223 E004 45.8791656 0.0058356714 8.854613e-10 1.325861e-08 2 68042714 68042831 118 - 1.470 1.880 1.395
ENSG00000197223 E005 167.7311228 0.0002077301 2.956993e-03 1.013521e-02 2 68042832 68043053 222 - 2.196 2.137 -0.195
ENSG00000197223 E006 162.6627629 0.0003197907 4.768219e-04 2.071383e-03 2 68045988 68046043 56 - 2.187 2.108 -0.264
ENSG00000197223 E007 3.4825944 0.0069520421 4.679778e-01 6.074103e-01 2 68046044 68046343 300 - 0.628 0.525 -0.464
ENSG00000197223 E008 148.3192887 0.0021667659 3.668216e-02 8.487208e-02 2 68046344 68046410 67 - 2.141 2.092 -0.163
ENSG00000197223 E009 1.0611596 0.0138658943 5.143054e-01 6.490252e-01 2 68046411 68046568 158 - 0.318 0.201 -0.880
ENSG00000197223 E010 211.8481674 0.0001889825 1.004640e-03 3.974731e-03 2 68047173 68047276 104 - 2.296 2.239 -0.187
ENSG00000197223 E011 153.9809877 0.0002799478 6.670799e-05 3.641863e-04 2 68047277 68047315 39 - 2.166 2.067 -0.331
ENSG00000197223 E012 135.9537865 0.0003257396 5.528644e-05 3.080816e-04 2 68047316 68047319 4 - 2.114 2.004 -0.371
ENSG00000197223 E013 25.7106466 0.0016541396 1.297040e-02 3.584176e-02 2 68053061 68053137 77 - 1.419 1.242 -0.618
ENSG00000197223 E014 0.2966881 0.0290785164 7.959033e-01   2 68053138 68053286 149 - 0.134 0.000 -8.661
ENSG00000197223 E015 57.9248999 0.0004622794 5.143217e-02 1.117003e-01 2 68062945 68062957 13 - 1.738 1.665 -0.248
ENSG00000197223 E016 132.8270697 0.0002872501 3.645582e-06 2.678682e-05 2 68062958 68063009 52 - 2.109 1.975 -0.451
ENSG00000197223 E017 0.5975289 0.0449125416 3.064467e-01 4.486161e-01 2 68110327 68110948 622 - 0.236 0.000 -9.662