ENSG00000196865

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369301 ENSG00000196865 HEK293_OSMI2_2hA HEK293_TMG_2hB NHLRC2 protein_coding protein_coding 5.902954 1.324923 8.916043 0.1044636 0.3463714 2.741264 3.488882 1.259919 5.358381 0.112992 0.1943382 2.079750 0.7319542 0.9496333 0.6014667 -0.3481667 9.291998e-10 1.166819e-15 FALSE TRUE
MSTRG.4667.1 ENSG00000196865 HEK293_OSMI2_2hA HEK293_TMG_2hB NHLRC2 protein_coding   5.902954 1.324923 8.916043 0.1044636 0.3463714 2.741264 2.271902 0.000000 3.358788 0.000000 0.2451530 8.396086 0.2208167 0.0000000 0.3758333 0.3758333 1.166819e-15 1.166819e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196865 E001 21.4783683 0.0008856995 1.017508e-02 2.919645e-02 10 113854661 113854823 163 + 1.286 1.080 -0.734
ENSG00000196865 E002 32.1635079 0.0012452114 4.812210e-03 1.540875e-02 10 113854824 113855050 227 + 1.451 1.270 -0.630
ENSG00000196865 E003 36.9048284 0.0030467769 1.116213e-03 4.357735e-03 10 113858528 113858680 153 + 1.515 1.302 -0.735
ENSG00000196865 E004 1.0725974 0.0235184942 2.684617e-02 6.567486e-02 10 113858681 113858852 172 + 0.156 0.594 2.757
ENSG00000196865 E005 70.3191979 0.0004443347 2.122816e-04 1.016508e-03 10 113876521 113876976 456 + 1.780 1.632 -0.503
ENSG00000196865 E006 34.2312480 0.0026923839 5.292286e-06 3.744986e-05 10 113879574 113879695 122 + 1.497 1.130 -1.286
ENSG00000196865 E007 39.2889115 0.0005866438 2.062247e-08 2.398026e-07 10 113884251 113884380 130 + 1.560 1.130 -1.502
ENSG00000196865 E008 36.5387086 0.0005981002 2.641881e-08 3.008334e-07 10 113898110 113898209 100 + 1.530 1.080 -1.578
ENSG00000196865 E009 52.2867812 0.0004683854 2.546209e-07 2.391293e-06 10 113901666 113901897 232 + 1.670 1.362 -1.054
ENSG00000196865 E010 31.5564462 0.0006034281 1.377497e-04 6.937361e-04 10 113902471 113902593 123 + 1.456 1.175 -0.982
ENSG00000196865 E011 49.3076427 0.0023630718 1.248495e-04 6.359871e-04 10 113903527 113903736 210 + 1.637 1.414 -0.765
ENSG00000196865 E012 53.8004343 0.0040930052 1.975490e-02 5.096782e-02 10 113904817 113905036 220 + 1.657 1.550 -0.364
ENSG00000196865 E013 51.6507523 0.0004313304 1.828624e-01 3.047766e-01 10 113908280 113908523 244 + 1.624 1.601 -0.079
ENSG00000196865 E014 713.2636556 0.0015157270 6.702800e-24 6.589649e-22 10 113908524 113917095 8572 + 2.710 2.892 0.607
ENSG00000196865 E015 16.5361087 0.0163417066 1.347760e-18 7.301376e-17 10 113917096 113917194 99 + 0.760 1.707 3.391
ENSG00000196865 E016 0.4418608 0.0245835353 7.142093e-01 8.104948e-01 10 113921049 113921118 70 + 0.156 0.001 -8.204
ENSG00000196865 E017 0.0000000       10 113926268 113926338 71 +