ENSG00000196814

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000361171 ENSG00000196814 HEK293_OSMI2_2hA HEK293_TMG_2hB MVB12B protein_coding protein_coding 6.936343 10.81595 6.259408 0.516358 0.2758305 -0.7880918 4.816163 4.523184 5.756366 0.9220026 0.2133962 0.3471377 0.7203208 0.4117333 0.9213333 0.5096000 1.833952e-06 1.017432e-20 FALSE TRUE
MSTRG.33395.3 ENSG00000196814 HEK293_OSMI2_2hA HEK293_TMG_2hB MVB12B protein_coding   6.936343 10.81595 6.259408 0.516358 0.2758305 -0.7880918 1.719580 5.523216 0.000000 0.4155878 0.0000000 -9.1119745 0.2210125 0.5166333 0.0000000 -0.5166333 1.017432e-20 1.017432e-20 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196814 E001 2.8378386 0.0054414757 2.175098e-06 1.677997e-05 9 126326829 126326861 33 + 0.872 0.193 -3.526
ENSG00000196814 E002 9.6175736 0.0019044164 4.984754e-03 1.588766e-02 9 126326862 126326940 79 + 1.118 0.918 -0.737
ENSG00000196814 E003 16.8518208 0.0012588573 4.872014e-05 2.753053e-04 9 126326941 126327010 70 + 1.361 1.124 -0.836
ENSG00000196814 E004 0.2955422 0.0298706774 6.909843e-01   9 126327011 126327015 5 + 0.140 0.107 -0.441
ENSG00000196814 E005 0.0000000       9 126335219 126335407 189 +      
ENSG00000196814 E006 43.6645977 0.0005353456 5.798404e-07 5.068240e-06 9 126340508 126340630 123 + 1.727 1.566 -0.547
ENSG00000196814 E007 57.1132004 0.0004538459 1.885988e-05 1.176744e-04 9 126381064 126381171 108 + 1.808 1.713 -0.322
ENSG00000196814 E008 65.4908314 0.0104157175 6.266132e-04 2.630634e-03 9 126386562 126386658 97 + 1.878 1.764 -0.387
ENSG00000196814 E009 73.1345552 0.0014884039 6.379383e-04 2.672848e-03 9 126392066 126392195 130 + 1.883 1.836 -0.160
ENSG00000196814 E010 51.0030747 0.0008111958 1.574369e-02 4.215801e-02 9 126395575 126395639 65 + 1.711 1.691 -0.066
ENSG00000196814 E011 43.4984726 0.0005383782 1.374332e-02 3.763777e-02 9 126395640 126395697 58 + 1.649 1.619 -0.103
ENSG00000196814 E012 8.9653093 0.0236232168 1.675587e-01 2.850127e-01 9 126395698 126397633 1936 + 0.800 1.082 1.062
ENSG00000196814 E013 51.8487423 0.0006051519 1.292295e-02 3.573253e-02 9 126421854 126421948 95 + 1.723 1.703 -0.069
ENSG00000196814 E014 1.6522545 0.0081333665 8.331592e-01 8.954708e-01 9 126448026 126448141 116 + 0.401 0.428 0.146
ENSG00000196814 E015 2.7959251 0.0056841074 1.202737e-01 2.203751e-01 9 126448142 126448409 268 + 0.330 0.666 1.677
ENSG00000196814 E016 0.5483223 0.0204239796 9.170759e-01 9.516047e-01 9 126480813 126481368 556 + 0.139 0.193 0.564
ENSG00000196814 E017 36.4792062 0.0006173477 2.287946e-02 5.749213e-02 9 126481369 126481424 56 + 1.577 1.546 -0.107
ENSG00000196814 E018 38.4344334 0.0046381056 1.683459e-01 2.860438e-01 9 126483973 126484032 60 + 1.568 1.581 0.044
ENSG00000196814 E019 900.0827935 0.0062103193 5.656937e-10 8.780744e-09 9 126503177 126507041 3865 + 2.796 3.011 0.713