ENSG00000196743

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000357164 ENSG00000196743 HEK293_OSMI2_2hA HEK293_TMG_2hB GM2A protein_coding protein_coding 29.18853 37.96377 21.52314 2.200915 0.3237082 -0.8184447 23.4503726 33.47011 16.027204 1.706500 0.4020882 -1.0618817 0.79138333 0.8823333 0.7444333 -0.13790000 7.236169e-09 7.236169e-09 FALSE TRUE
MSTRG.27183.2 ENSG00000196743 HEK293_OSMI2_2hA HEK293_TMG_2hB GM2A protein_coding   29.18853 37.96377 21.52314 2.200915 0.3237082 -0.8184447 0.4559136 0.00000 1.628411 0.000000 1.6284111 7.3561536 0.01905833 0.0000000 0.0742000 0.07420000 7.919837e-01 7.236169e-09 FALSE TRUE
MSTRG.27183.3 ENSG00000196743 HEK293_OSMI2_2hA HEK293_TMG_2hB GM2A protein_coding   29.18853 37.96377 21.52314 2.200915 0.3237082 -0.8184447 4.9012290 4.17924 3.644960 0.588148 1.8224999 -0.1968329 0.17635000 0.1091333 0.1711000 0.06196667 1.000000e+00 7.236169e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196743 E001 0.000000       5 151212150 151212259 110 +      
ENSG00000196743 E002 0.000000       5 151212759 151212889 131 +      
ENSG00000196743 E003 12.909996 0.0041474163 8.016391e-02 1.597973e-01 5 151253071 151253136 66 + 1.188 1.076 -0.400
ENSG00000196743 E004 39.352868 0.0005781897 1.757038e-03 6.462530e-03 5 151253137 151253184 48 + 1.650 1.534 -0.394
ENSG00000196743 E005 190.146649 0.0016835783 1.261806e-03 4.849284e-03 5 151253185 151253297 113 + 2.275 2.236 -0.131
ENSG00000196743 E006 177.359085 0.0019201422 3.447061e-04 1.558846e-03 5 151259755 151259798 44 + 2.256 2.201 -0.184
ENSG00000196743 E007 247.462992 0.0009929192 4.667930e-04 2.033119e-03 5 151259799 151259916 118 + 2.382 2.353 -0.099
ENSG00000196743 E008 299.390533 0.0020538971 2.978747e-04 1.370927e-03 5 151266731 151266913 183 + 2.472 2.432 -0.132
ENSG00000196743 E009 3.317181 0.0047092090 4.772990e-01 6.158840e-01 5 151266914 151267255 342 + 0.653 0.592 -0.263
ENSG00000196743 E010 326.705639 0.0004812392 1.177759e-07 1.180732e-06 5 151267296 151267480 185 + 2.517 2.467 -0.167
ENSG00000196743 E011 121.905737 0.0003254455 1.280102e-01 2.313865e-01 5 151267481 151267482 2 + 2.051 2.063 0.043
ENSG00000196743 E012 2079.766432 0.0024963804 2.440643e-12 5.620183e-11 5 151267483 151270149 2667 + 3.178 3.328 0.500
ENSG00000196743 E013 44.493911 0.0140455113 3.145828e-04 1.439223e-03 5 151270150 151270440 291 + 1.762 1.550 -0.719