ENSG00000196584

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000359321 ENSG00000196584 HEK293_OSMI2_2hA HEK293_TMG_2hB XRCC2 protein_coding protein_coding 14.23366 8.037213 20.46374 0.3529104 0.6859994 1.347214 9.767024 2.5777076 15.4665987 0.4878645 0.3604803 2.5803419 0.61737083 0.31663333 0.75636667 0.43973333 1.479685e-10 1.479685e-10 FALSE TRUE
MSTRG.30958.3 ENSG00000196584 HEK293_OSMI2_2hA HEK293_TMG_2hB XRCC2 protein_coding   14.23366 8.037213 20.46374 0.3529104 0.6859994 1.347214 1.141685 1.2387496 1.4668956 0.1540262 0.4104094 0.2420837 0.09119583 0.15523333 0.07083333 -0.08440000 1.289933e-01 1.479685e-10 FALSE FALSE
MSTRG.30958.4 ENSG00000196584 HEK293_OSMI2_2hA HEK293_TMG_2hB XRCC2 protein_coding   14.23366 8.037213 20.46374 0.3529104 0.6859994 1.347214 1.276721 0.3803015 2.4952603 0.1794973 0.4663005 2.6822996 0.07343750 0.04786667 0.12183333 0.07396667 1.762811e-01 1.479685e-10 FALSE FALSE
MSTRG.30958.6 ENSG00000196584 HEK293_OSMI2_2hA HEK293_TMG_2hB XRCC2 protein_coding   14.23366 8.037213 20.46374 0.3529104 0.6859994 1.347214 1.629221 3.5516032 0.5144902 0.1745837 0.3313646 -2.7635390 0.19007500 0.44523333 0.02593333 -0.41930000 6.485205e-03 1.479685e-10   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196584 E001 29.3131399 0.0096976689 7.037360e-01 8.023970e-01 7 152644776 152644778 3 - 1.470 1.374 -0.334
ENSG00000196584 E002 929.8720537 0.0146455196 1.415991e-07 1.397745e-06 7 152644779 152648919 4141 - 2.988 2.741 -0.821
ENSG00000196584 E003 191.7144108 0.0005767856 6.258638e-04 2.627837e-03 7 152648920 152649363 444 - 2.302 2.061 -0.807
ENSG00000196584 E004 7.4620380 0.0225028280 1.403023e-15 5.205611e-14 7 152656772 152656883 112 - 0.369 1.329 3.926
ENSG00000196584 E005 26.5373857 0.0105248783 1.932700e-32 3.874543e-30 7 152656975 152657110 136 - 0.947 1.810 3.018
ENSG00000196584 E006 29.4740101 0.0262171268 1.798539e-19 1.083479e-17 7 152657111 152657232 122 - 1.055 1.830 2.687
ENSG00000196584 E007 85.5621834 0.0003509503 1.803611e-09 2.547824e-08 7 152660701 152660782 82 - 1.860 1.973 0.381
ENSG00000196584 E008 0.4407149 0.0230511077 5.774354e-01 7.027038e-01 7 152663944 152664004 61 - 0.141 0.191 0.530
ENSG00000196584 E009 71.5715551 0.0014133955 8.994458e-08 9.207679e-07 7 152676041 152676183 143 - 1.782 1.901 0.402