ENSG00000196526

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000358461 ENSG00000196526 HEK293_OSMI2_2hA HEK293_TMG_2hB AFAP1 protein_coding protein_coding 4.703928 4.41677 4.888802 0.1014503 0.1137306 0.1461746 2.034301 0.9306485 4.2923886 0.04768475 0.6672107 2.193410 0.4210958 0.2112667 0.8726 0.6613333 0.01393381 0.01393381 FALSE TRUE
ENST00000360265 ENSG00000196526 HEK293_OSMI2_2hA HEK293_TMG_2hB AFAP1 protein_coding protein_coding 4.703928 4.41677 4.888802 0.1014503 0.1137306 0.1461746 2.521755 3.2125449 0.5519003 0.12684048 0.5519003 -2.519814 0.5465250 0.7269667 0.1183 -0.6086667 0.03183893 0.01393381 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196526 E001 0.1482932 0.0414566846 4.440083e-01   4 7758714 7758714 1 - 0.000 0.134 9.972
ENSG00000196526 E002 18.4568953 0.0019396328 1.014774e-16 4.362283e-15 4 7758715 7758927 213 - 0.706 1.537 3.033
ENSG00000196526 E003 594.3927155 0.0029093486 3.309880e-12 7.461290e-11 4 7758928 7763221 4294 - 2.679 2.855 0.587
ENSG00000196526 E004 60.4798404 0.0004568043 2.651620e-02 6.498283e-02 4 7763222 7763566 345 - 1.812 1.764 -0.163
ENSG00000196526 E005 47.6291088 0.0005143548 8.573939e-01 9.119327e-01 4 7763567 7763791 225 - 1.667 1.709 0.140
ENSG00000196526 E006 30.4302615 0.0006677081 9.145727e-01 9.499383e-01 4 7768844 7769008 165 - 1.467 1.523 0.193
ENSG00000196526 E007 40.9288795 0.0005847503 6.492172e-01 7.605400e-01 4 7772820 7773010 191 - 1.609 1.635 0.088
ENSG00000196526 E008 44.3548396 0.0005528564 3.914706e-01 5.353874e-01 4 7774739 7774903 165 - 1.651 1.656 0.019
ENSG00000196526 E009 34.0425883 0.0006000487 3.220718e-01 4.653543e-01 4 7778762 7778876 115 - 1.545 1.537 -0.029
ENSG00000196526 E010 0.1472490 0.0430715200 5.372239e-01   4 7778877 7779187 311 - 0.111 0.000 -12.355
ENSG00000196526 E011 1.5113482 0.0098559068 2.525822e-01 3.889561e-01 4 7781376 7781627 252 - 0.273 0.502 1.317
ENSG00000196526 E012 32.7927391 0.0026606005 3.203938e-02 7.592995e-02 4 7786194 7786311 118 - 1.570 1.483 -0.298
ENSG00000196526 E013 0.1817044 0.0398938879 4.421892e-01   4 7788607 7789020 414 - 0.000 0.135 12.017
ENSG00000196526 E014 37.0141369 0.0111743685 7.638086e-02 1.537118e-01 4 7793681 7793826 146 - 1.615 1.531 -0.286
ENSG00000196526 E015 0.0000000       4 7798900 7799160 261 -      
ENSG00000196526 E016 46.3134374 0.0005356633 1.367024e-03 5.199469e-03 4 7800442 7800653 212 - 1.726 1.612 -0.385
ENSG00000196526 E017 0.3697384 0.0247685778 1.731973e-01 2.923510e-01 4 7809439 7809613 175 - 0.000 0.237 13.144
ENSG00000196526 E018 35.8099565 0.0013532678 2.786167e-04 1.292753e-03 4 7809614 7809763 150 - 1.639 1.473 -0.569
ENSG00000196526 E019 25.6287862 0.0007683637 2.721962e-03 9.438237e-03 4 7816018 7816099 82 - 1.492 1.337 -0.536
ENSG00000196526 E020 29.8366258 0.0006547645 1.902941e-03 6.923628e-03 4 7819076 7819171 96 - 1.556 1.410 -0.502
ENSG00000196526 E021 24.3353297 0.0007988396 5.866107e-02 1.243371e-01 4 7838524 7838587 64 - 1.440 1.358 -0.283
ENSG00000196526 E022 29.5085022 0.0006747448 2.882765e-03 9.919018e-03 4 7838588 7838703 116 - 1.549 1.410 -0.478
ENSG00000196526 E023 0.1482932 0.0414566846 4.440083e-01   4 7842688 7843138 451 - 0.000 0.134 12.001
ENSG00000196526 E024 33.2522281 0.0008505706 1.733581e-02 4.568012e-02 4 7843139 7843350 212 - 1.577 1.483 -0.320
ENSG00000196526 E025 0.0000000       4 7843743 7843837 95 -      
ENSG00000196526 E026 22.7672405 0.0093941564 5.630542e-02 1.202513e-01 4 7855466 7855574 109 - 1.426 1.315 -0.384
ENSG00000196526 E027 0.2987644 0.0273044922 2.294672e-01   4 7861985 7862311 327 - 0.200 0.000 -13.503
ENSG00000196526 E028 24.5442357 0.0012275254 1.334945e-03 5.093067e-03 4 7868622 7868719 98 - 1.484 1.307 -0.613
ENSG00000196526 E029 23.6174917 0.0008480732 3.471912e-05 2.031891e-04 4 7871952 7872080 129 - 1.496 1.248 -0.860
ENSG00000196526 E030 0.0000000       4 7872081 7872294 214 -      
ENSG00000196526 E031 16.6820846 0.0048007305 2.977716e-06 2.229633e-05 4 7939656 7939926 271 - 1.396 1.019 -1.340