ENSG00000196476

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000360321 ENSG00000196476 HEK293_OSMI2_2hA HEK293_TMG_2hB C20orf96 protein_coding protein_coding 8.146338 11.52674 5.133675 0.1871449 0.09694277 -1.165364 0.688157 0.7795505 0.8094093 0.06162618 0.19583134 0.05355273 0.0945875 0.0675000 0.1589333 0.09143333 0.1100251734 0.0008904212 FALSE TRUE
ENST00000382369 ENSG00000196476 HEK293_OSMI2_2hA HEK293_TMG_2hB C20orf96 protein_coding protein_coding 8.146338 11.52674 5.133675 0.1871449 0.09694277 -1.165364 4.516253 7.1552848 2.3163529 0.28009216 0.02268166 -1.62295406 0.5328708 0.6205000 0.4515667 -0.16893333 0.0008904212 0.0008904212 FALSE TRUE
MSTRG.20218.4 ENSG00000196476 HEK293_OSMI2_2hA HEK293_TMG_2hB C20orf96 protein_coding   8.146338 11.52674 5.133675 0.1871449 0.09694277 -1.165364 2.938289 3.5919036 2.0079125 0.17317034 0.27109757 -0.83589594 0.3719250 0.3119667 0.3895667 0.07760000 0.4817482054 0.0008904212 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196476 E001 6.382610 0.0027157253 0.610614100 0.72992570 20 270863 270924 62 - 0.755 0.825 0.277
ENSG00000196476 E002 69.471370 0.0003972673 0.050882656 0.11074258 20 270925 271267 343 - 1.712 1.804 0.311
ENSG00000196476 E003 54.706182 0.0004430917 0.288539543 0.42914302 20 275968 276048 81 - 1.636 1.691 0.189
ENSG00000196476 E004 33.037645 0.0006673203 0.790168854 0.86579517 20 276049 276086 38 - 1.449 1.466 0.059
ENSG00000196476 E005 46.712341 0.0006060877 0.141694877 0.25035365 20 276793 276879 87 - 1.543 1.628 0.291
ENSG00000196476 E006 55.377069 0.0004997248 0.010033616 0.02884455 20 277044 277145 102 - 1.574 1.714 0.477
ENSG00000196476 E007 72.411507 0.0003643104 0.655128018 0.76508037 20 277226 277383 158 - 1.785 1.805 0.066
ENSG00000196476 E008 68.083232 0.0004480295 0.889177421 0.93314865 20 278330 278429 100 - 1.770 1.776 0.020
ENSG00000196476 E009 78.429715 0.0005660248 0.480058570 0.61836954 20 279172 279330 159 - 1.854 1.822 -0.105
ENSG00000196476 E010 78.166531 0.0003664807 0.007638897 0.02287033 20 283963 284081 119 - 1.913 1.802 -0.374
ENSG00000196476 E011 55.876041 0.0017114678 0.028161611 0.06833168 20 289559 289676 118 - 1.778 1.665 -0.383
ENSG00000196476 E012 68.996664 0.0004776927 0.118952386 0.21843816 20 290259 290307 49 - 1.834 1.764 -0.238
ENSG00000196476 E013 63.137872 0.0005047382 0.350999314 0.49519863 20 290308 290429 122 - 1.708 1.753 0.153
ENSG00000196476 E014 0.000000       20 290587 290590 4 -      
ENSG00000196476 E015 7.092569 0.0028808222 0.026280560 0.06451026 20 290591 290778 188 - 1.038 0.769 -1.023