ENSG00000196455

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000356763 ENSG00000196455 HEK293_OSMI2_2hA HEK293_TMG_2hB PIK3R4 protein_coding protein_coding 18.13525 13.33241 19.44056 1.835553 0.09840533 0.5437925 13.0954107 4.604435 17.9722966 0.1550845 0.5091292 1.9623514 0.65460833 0.3629667 0.92456667 0.56160000 1.606565e-08 2.423778e-33 FALSE TRUE
ENST00000511760 ENSG00000196455 HEK293_OSMI2_2hA HEK293_TMG_2hB PIK3R4 protein_coding retained_intron 18.13525 13.33241 19.44056 1.835553 0.09840533 0.5437925 1.7851057 4.958301 0.0000000 1.1844848 0.0000000 -8.9566089 0.13675833 0.3630667 0.00000000 -0.36306667 2.423778e-33 2.423778e-33 FALSE TRUE
ENST00000512362 ENSG00000196455 HEK293_OSMI2_2hA HEK293_TMG_2hB PIK3R4 protein_coding retained_intron 18.13525 13.33241 19.44056 1.835553 0.09840533 0.5437925 2.6928577 2.355573 1.3296899 1.2633256 0.3923696 -0.8202906 0.16830833 0.1546667 0.06833333 -0.08633333 9.260597e-01 2.423778e-33 FALSE TRUE
ENST00000512677 ENSG00000196455 HEK293_OSMI2_2hA HEK293_TMG_2hB PIK3R4 protein_coding processed_transcript 18.13525 13.33241 19.44056 1.835553 0.09840533 0.5437925 0.5246911 1.414102 0.1385769 0.7257915 0.1385769 -3.2607709 0.03886667 0.1193000 0.00710000 -0.11220000 4.030356e-01 2.423778e-33 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196455 E001 14.1039608 0.0013227934 2.985082e-07 2.764137e-06 3 130678934 130679009 76 - 0.951 1.416 1.658
ENSG00000196455 E002 162.6687241 0.0037413967 1.342429e-21 1.023829e-19 3 130679010 130679485 476 - 2.025 2.409 1.285
ENSG00000196455 E003 2.0023880 0.0219565669 3.085817e-01 4.508672e-01 3 130680521 130680603 83 - 0.378 0.565 0.944
ENSG00000196455 E004 1.1823698 0.0119477040 3.883900e-01 5.324619e-01 3 130680604 130680612 9 - 0.254 0.415 1.013
ENSG00000196455 E005 110.1942467 0.0040922949 3.765484e-10 6.033151e-09 3 130680613 130680721 109 - 1.902 2.195 0.982
ENSG00000196455 E006 100.8448901 0.0044444759 1.122058e-06 9.218948e-06 3 130680977 130681065 89 - 1.887 2.128 0.808
ENSG00000196455 E007 0.7708142 0.0153787590 7.121646e-01 8.089886e-01 3 130681066 130681071 6 - 0.254 0.186 -0.572
ENSG00000196455 E008 107.7885651 0.0021432550 1.814848e-05 1.136925e-04 3 130681491 130681591 101 - 1.939 2.124 0.618
ENSG00000196455 E009 86.9527948 0.0003480411 4.875730e-06 3.480711e-05 3 130684250 130684305 56 - 1.844 2.032 0.631
ENSG00000196455 E010 91.3184820 0.0004625809 2.751643e-04 1.278618e-03 3 130684306 130684381 76 - 1.880 2.030 0.505
ENSG00000196455 E011 182.4202414 0.0002214497 1.237828e-05 8.049665e-05 3 130686211 130686422 212 - 2.187 2.315 0.426
ENSG00000196455 E012 154.0621357 0.0002465794 1.051881e-03 4.139018e-03 3 130690490 130690654 165 - 2.122 2.228 0.355
ENSG00000196455 E013 0.0000000       3 130690655 130690657 3 -      
ENSG00000196455 E014 135.6210268 0.0002421612 1.079541e-01 2.024948e-01 3 130703723 130703888 166 - 2.085 2.145 0.200
ENSG00000196455 E015 143.5448229 0.0002486563 7.861053e-01 8.630053e-01 3 130705561 130705771 211 - 2.122 2.138 0.056
ENSG00000196455 E016 130.6016154 0.0003136423 3.751274e-01 5.195357e-01 3 130706948 130707135 188 - 2.094 2.071 -0.075
ENSG00000196455 E017 66.2095892 0.0005069739 4.005814e-03 1.315826e-02 3 130708291 130708321 31 - 1.831 1.695 -0.458
ENSG00000196455 E018 126.5344161 0.0002587780 1.745951e-02 4.595430e-02 3 130708322 130708492 171 - 2.094 2.019 -0.252
ENSG00000196455 E019 49.9637087 0.0004546309 1.094086e-01 2.046901e-01 3 130716396 130716416 21 - 1.699 1.618 -0.274
ENSG00000196455 E020 42.4816197 0.0007492970 1.553061e-01 2.688021e-01 3 130716417 130716423 7 - 1.630 1.552 -0.266
ENSG00000196455 E021 112.0729815 0.0002497592 8.656833e-04 3.493324e-03 3 130716424 130716599 176 - 2.054 1.936 -0.394
ENSG00000196455 E022 91.3513603 0.0002950248 5.491277e-05 3.062335e-04 3 130718389 130718530 142 - 1.976 1.813 -0.549
ENSG00000196455 E023 37.0748561 0.0005856849 3.091076e-03 1.053399e-02 3 130718531 130718534 4 - 1.603 1.416 -0.642
ENSG00000196455 E024 90.7689938 0.0025673529 3.137847e-03 1.067073e-02 3 130723414 130723587 174 - 1.967 1.831 -0.459
ENSG00000196455 E025 0.0000000       3 130723694 130723810 117 -      
ENSG00000196455 E026 0.0000000       3 130723811 130723893 83 -      
ENSG00000196455 E027 0.0000000       3 130725899 130725958 60 -      
ENSG00000196455 E028 100.6314361 0.0002701720 2.256650e-02 5.685652e-02 3 130728463 130728684 222 - 1.998 1.917 -0.273
ENSG00000196455 E029 78.4076818 0.0003340712 3.926784e-02 8.965669e-02 3 130730308 130730442 135 - 1.890 1.806 -0.283
ENSG00000196455 E030 196.4135273 0.0005483863 2.637952e-10 4.326108e-09 3 130733548 130734130 583 - 2.311 2.125 -0.620
ENSG00000196455 E031 75.0627469 0.0003566347 8.309713e-08 8.574814e-07 3 130735869 130736002 134 - 1.913 1.661 -0.850
ENSG00000196455 E032 211.6284071 0.0051972893 2.875927e-12 6.548507e-11 3 130744486 130745264 779 - 2.370 2.060 -1.035
ENSG00000196455 E033 110.0841259 0.0002918821 1.103111e-20 7.670430e-19 3 130746318 130746829 512 - 2.102 1.713 -1.308