ENSG00000196411

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000358173 ENSG00000196411 HEK293_OSMI2_2hA HEK293_TMG_2hB EPHB4 protein_coding protein_coding 46.63801 60.09421 38.75965 4.517776 1.091602 -0.6325383 32.24954 44.12922 24.94399 4.046562 0.1703463 -0.82279021 0.6836208 0.7324000 0.6446000 -0.0878000 0.04733303 0.01298667 FALSE TRUE
ENST00000478459 ENSG00000196411 HEK293_OSMI2_2hA HEK293_TMG_2hB EPHB4 protein_coding retained_intron 46.63801 60.09421 38.75965 4.517776 1.091602 -0.6325383 13.35752 13.48264 13.15222 2.064004 0.9344153 -0.03576999 0.2947083 0.2215667 0.3385333 0.1169667 0.01298667 0.01298667 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196411 E001 0.7331471 0.0172671820 1.391072e-01 2.468400e-01 7 100802565 100802567 3 - 0.000 0.290 9.881
ENSG00000196411 E002 994.5870780 0.0050057954 3.774994e-08 4.168822e-07 7 100802568 100803238 671 - 2.809 3.016 0.688
ENSG00000196411 E003 775.3508967 0.0033776720 7.948297e-05 4.254460e-04 7 100803239 100803590 352 - 2.754 2.891 0.456
ENSG00000196411 E004 507.2659585 0.0001713003 2.352034e-09 3.255065e-08 7 100805166 100805321 156 - 2.581 2.701 0.401
ENSG00000196411 E005 610.0980298 0.0001565289 4.558657e-06 3.275397e-05 7 100805501 100805694 194 - 2.685 2.772 0.292
ENSG00000196411 E006 1.1007972 0.1479445946 9.526103e-01 9.743240e-01 7 100805973 100806417 445 - 0.277 0.294 0.121
ENSG00000196411 E007 1.1854902 0.0189120224 6.354810e-01 7.497792e-01 7 100806418 100806419 2 - 0.369 0.290 -0.495
ENSG00000196411 E008 549.3156884 0.0001505398 4.376461e-02 9.795808e-02 7 100806420 100806569 150 - 2.669 2.716 0.157
ENSG00000196411 E009 673.9700213 0.0001570656 4.761827e-01 6.148415e-01 7 100807365 100807580 216 - 2.788 2.790 0.009
ENSG00000196411 E010 275.6081832 0.0002121502 7.668777e-01 8.492488e-01 7 100812747 100812784 38 - 2.397 2.403 0.020
ENSG00000196411 E011 615.6453608 0.0001221513 1.349397e-01 2.410138e-01 7 100812785 100812994 210 - 2.757 2.747 -0.033
ENSG00000196411 E012 446.0735285 0.0001228046 7.044821e-04 2.915100e-03 7 100813095 100813208 114 - 2.644 2.596 -0.159
ENSG00000196411 E013 0.6621601 0.0164040157 5.172641e-01 6.516812e-01 7 100813209 100813339 131 - 0.277 0.169 -0.910
ENSG00000196411 E014 349.4821910 0.0001538369 6.342682e-07 5.497035e-06 7 100813652 100813716 65 - 2.563 2.476 -0.291
ENSG00000196411 E015 315.9772576 0.0001624021 1.187619e-06 9.707083e-06 7 100813919 100813988 70 - 2.522 2.433 -0.299
ENSG00000196411 E016 223.4359433 0.0026114441 4.846050e-03 1.550322e-02 7 100813989 100814021 33 - 2.370 2.287 -0.280
ENSG00000196411 E017 5.4542453 0.0031161828 2.641415e-01 4.020926e-01 7 100814022 100814088 67 - 0.860 0.722 -0.547
ENSG00000196411 E018 239.5767523 0.0005322724 1.559410e-02 4.183313e-02 7 100817192 100817244 53 - 2.377 2.327 -0.166
ENSG00000196411 E019 150.9998604 0.0017024579 3.295281e-01 4.730477e-01 7 100817245 100817261 17 - 2.158 2.136 -0.072
ENSG00000196411 E020 254.5008228 0.0049875314 4.204013e-01 5.636114e-01 7 100817262 100817357 96 - 2.383 2.365 -0.057
ENSG00000196411 E021 172.5752543 0.0058029988 4.345066e-01 5.768706e-01 7 100818520 100818550 31 - 2.215 2.195 -0.066
ENSG00000196411 E022 259.5697112 0.0024633676 1.738017e-01 2.931309e-01 7 100818551 100818644 94 - 2.399 2.369 -0.100
ENSG00000196411 E023 489.7513356 0.0001716637 3.073547e-04 1.409387e-03 7 100819557 100819889 333 - 2.685 2.636 -0.165
ENSG00000196411 E024 244.3283949 0.0001729968 2.815827e-01 4.214897e-01 7 100820141 100820296 156 - 2.359 2.346 -0.045
ENSG00000196411 E025 1.1447303 0.1889702598 8.055987e-01 8.764054e-01 7 100820297 100820482 186 - 0.278 0.337 0.390
ENSG00000196411 E026 389.0287664 0.0020696908 7.415643e-02 1.501926e-01 7 100822271 100822667 397 - 2.576 2.542 -0.114
ENSG00000196411 E027 297.3881584 0.0069390625 1.622145e-02 4.321318e-02 7 100823644 100823931 288 - 2.496 2.408 -0.291
ENSG00000196411 E028 145.5508228 0.0045396942 1.054608e-04 5.471663e-04 7 100824203 100824273 71 - 2.226 2.072 -0.515
ENSG00000196411 E029 3.7462405 0.0046620817 8.247916e-01 8.896180e-01 7 100824274 100825526 1253 - 0.615 0.659 0.189
ENSG00000196411 E030 201.2541208 0.0121752762 1.080425e-06 8.903534e-06 7 100826979 100827523 545 - 2.433 2.169 -0.882