ENSG00000196405

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392920 ENSG00000196405 HEK293_OSMI2_2hA HEK293_TMG_2hB EVL protein_coding protein_coding 53.21048 85.65786 36.30962 2.142176 1.930807 -1.238005 22.933424 47.057421 11.174322 2.2506214 1.24413798 -2.0732510 0.40413333 0.54936667 0.31303333 -0.23633333 7.506075e-03 2.025297e-05 FALSE TRUE
ENST00000402714 ENSG00000196405 HEK293_OSMI2_2hA HEK293_TMG_2hB EVL protein_coding protein_coding 53.21048 85.65786 36.30962 2.142176 1.930807 -1.238005 6.056785 6.036315 7.998376 1.0708486 0.53898659 0.4054537 0.12226250 0.07040000 0.22160000 0.15120000 2.025297e-05 2.025297e-05 FALSE TRUE
ENST00000544450 ENSG00000196405 HEK293_OSMI2_2hA HEK293_TMG_2hB EVL protein_coding protein_coding 53.21048 85.65786 36.30962 2.142176 1.930807 -1.238005 3.784069 2.938887 2.658063 0.3846044 0.17924316 -0.1443775 0.08223750 0.03413333 0.07310000 0.03896667 3.545229e-05 2.025297e-05 FALSE TRUE
ENST00000554695 ENSG00000196405 HEK293_OSMI2_2hA HEK293_TMG_2hB EVL protein_coding protein_coding 53.21048 85.65786 36.30962 2.142176 1.930807 -1.238005 3.411254 8.312290 1.436682 0.1905438 0.30557028 -2.5242329 0.05071250 0.09726667 0.03960000 -0.05766667 1.376554e-03 2.025297e-05 FALSE TRUE
ENST00000555848 ENSG00000196405 HEK293_OSMI2_2hA HEK293_TMG_2hB EVL protein_coding retained_intron 53.21048 85.65786 36.30962 2.142176 1.930807 -1.238005 5.944017 6.181393 3.332183 0.9795791 0.09104194 -0.8894732 0.11951667 0.07170000 0.09233333 0.02063333 4.421691e-01 2.025297e-05   FALSE
ENST00000557153 ENSG00000196405 HEK293_OSMI2_2hA HEK293_TMG_2hB EVL protein_coding protein_coding 53.21048 85.65786 36.30962 2.142176 1.930807 -1.238005 1.246245 0.000000 3.366156 0.0000000 1.71174187 8.3992376 0.03540000 0.00000000 0.08803333 0.08803333 1.128347e-01 2.025297e-05 FALSE FALSE
MSTRG.10168.2 ENSG00000196405 HEK293_OSMI2_2hA HEK293_TMG_2hB EVL protein_coding   53.21048 85.65786 36.30962 2.142176 1.930807 -1.238005 4.089366 9.935618 1.845823 3.2552669 0.66304785 -2.4220020 0.06390417 0.11613333 0.05046667 -0.06566667 3.779034e-01 2.025297e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196405 E001 65.7316259 0.0297030056 2.422743e-10 4.000535e-09 14 99971449 99971984 536 + 2.119 1.523 -2.015
ENSG00000196405 E002 60.8578935 0.0004736760 2.595863e-38 8.035596e-36 14 99971985 99972057 73 + 2.086 1.491 -2.011
ENSG00000196405 E003 0.3332198 0.0289862779 4.538117e-01   14 100019382 100019575 194 + 0.190 0.077 -1.500
ENSG00000196405 E004 0.4804688 0.0210188592 1.480228e-01 2.589835e-01 14 100019576 100019687 112 + 0.321 0.077 -2.500
ENSG00000196405 E005 0.0000000       14 100065417 100065420 4 +      
ENSG00000196405 E006 0.0000000       14 100065421 100065421 1 +      
ENSG00000196405 E007 0.0000000       14 100065422 100065424 3 +      
ENSG00000196405 E008 0.0000000       14 100065425 100065429 5 +      
ENSG00000196405 E009 0.0000000       14 100065430 100065431 2 +      
ENSG00000196405 E010 0.0000000       14 100065432 100065444 13 +      
ENSG00000196405 E011 0.0000000       14 100065445 100065511 67 +      
ENSG00000196405 E012 0.0000000       14 100066437 100066487 51 +      
ENSG00000196405 E013 0.0000000       14 100074503 100074615 113 +      
ENSG00000196405 E014 258.9337704 0.0002113558 3.519097e-17 1.599142e-15 14 100084687 100084830 144 + 2.486 2.279 -0.692
ENSG00000196405 E015 198.7552838 0.0004033554 9.228211e-06 6.183923e-05 14 100084831 100084855 25 + 2.318 2.186 -0.442
ENSG00000196405 E016 0.8438776 0.3320696616 5.209215e-01 6.548575e-01 14 100084856 100085098 243 + 0.321 0.200 -0.905
ENSG00000196405 E017 0.2965864 0.1995327994 7.363833e-01   14 100096947 100097045 99 + 0.000 0.140 9.166
ENSG00000196405 E018 454.8383355 0.0013827629 9.738597e-05 5.097809e-04 14 100097481 100097658 178 + 2.656 2.555 -0.336
ENSG00000196405 E019 0.4031496 0.0251625706 6.586206e-01 7.676776e-01 14 100097659 100097745 87 + 0.000 0.142 10.257
ENSG00000196405 E020 4.4244126 0.0036764765 3.980044e-04 1.766611e-03 14 100102304 100102379 76 + 0.989 0.496 -2.040
ENSG00000196405 E021 0.2944980 0.3469355977 7.899111e-02   14 100102380 100102425 46 + 0.321 0.000 -11.598
ENSG00000196405 E022 11.9286461 0.0015737689 2.784911e-02 6.771654e-02 14 100106024 100106825 802 + 1.198 0.970 -0.828
ENSG00000196405 E023 18.4728984 0.0453006142 5.155786e-01 6.502356e-01 14 100106826 100108106 1281 + 1.150 1.244 0.335
ENSG00000196405 E024 0.0000000       14 100114530 100114591 62 +      
ENSG00000196405 E025 242.8963579 0.0012623346 5.265744e-02 1.138854e-01 14 100123539 100123602 64 + 2.356 2.293 -0.211
ENSG00000196405 E026 162.2772165 0.0016211201 8.216074e-01 8.874831e-01 14 100126707 100126771 65 + 2.131 2.134 0.013
ENSG00000196405 E027 71.0373653 0.0003900400 7.287434e-02 1.481232e-01 14 100128519 100128586 68 + 1.711 1.796 0.290
ENSG00000196405 E028 11.6874360 0.0028138210 1.257530e-01 2.282209e-01 14 100128587 100128593 7 + 0.879 1.059 0.671
ENSG00000196405 E029 34.9697248 0.0069809361 1.392404e-01 2.470144e-01 14 100128594 100128620 27 + 1.380 1.499 0.410
ENSG00000196405 E030 263.0310869 0.0005182871 9.694154e-01 9.848766e-01 14 100128621 100128748 128 + 2.345 2.340 -0.019
ENSG00000196405 E031 481.8493759 0.0001241762 8.655385e-01 9.173814e-01 14 100129563 100129684 122 + 2.611 2.603 -0.025
ENSG00000196405 E032 459.0914321 0.0002292601 2.954874e-01 4.366143e-01 14 100132719 100132779 61 + 2.573 2.589 0.056
ENSG00000196405 E033 2.9914357 0.0057209894 4.231692e-01 5.662798e-01 14 100134653 100134867 215 + 0.632 0.496 -0.624
ENSG00000196405 E034 7.4920337 0.0022413150 3.970682e-03 1.305984e-02 14 100135008 100135522 515 + 1.097 0.752 -1.307
ENSG00000196405 E035 3.7693091 0.1780688152 4.496217e-01 5.908181e-01 14 100135771 100135904 134 + 0.773 0.565 -0.884
ENSG00000196405 E036 470.5351153 0.0001501024 3.309090e-02 7.799168e-02 14 100135905 100135968 64 + 2.566 2.603 0.123
ENSG00000196405 E037 9.9298653 0.0346142027 1.371548e-03 5.213605e-03 14 100137106 100137577 472 + 1.255 0.823 -1.588
ENSG00000196405 E038 512.1540132 0.0001946614 1.036043e-03 4.082945e-03 14 100137578 100137644 67 + 2.586 2.644 0.194
ENSG00000196405 E039 533.9637118 0.0001410921 2.227453e-02 5.624283e-02 14 100137740 100137802 63 + 2.621 2.658 0.124
ENSG00000196405 E040 99.1747048 0.0088825704 1.227395e-12 2.970822e-11 14 100137803 100138567 765 + 2.202 1.787 -1.396
ENSG00000196405 E041 42.0359473 0.0055455136 6.958033e-05 3.779723e-04 14 100138568 100138811 244 + 1.746 1.479 -0.912
ENSG00000196405 E042 20.0407469 0.0414850546 2.370642e-02 5.920729e-02 14 100138812 100138897 86 + 1.453 1.162 -1.020
ENSG00000196405 E043 46.1191950 0.0081486863 5.447952e-05 3.041218e-04 14 100138898 100139333 436 + 1.795 1.512 -0.961
ENSG00000196405 E044 14.8618486 0.0042601093 7.488502e-04 3.074502e-03 14 100139334 100139369 36 + 1.349 1.029 -1.140
ENSG00000196405 E045 85.4051849 0.0066030882 2.906721e-06 2.181647e-05 14 100139370 100140117 748 + 2.043 1.779 -0.888
ENSG00000196405 E046 135.5375853 0.0054143699 1.268933e-04 6.451319e-04 14 100140293 100141179 887 + 2.190 2.005 -0.618
ENSG00000196405 E047 562.3010219 0.0002062560 1.575173e-03 5.878879e-03 14 100141180 100141246 67 + 2.629 2.683 0.178
ENSG00000196405 E048 531.5306692 0.0001954800 1.330899e-05 8.591210e-05 14 100141736 100141793 58 + 2.587 2.665 0.260
ENSG00000196405 E049 18.4174540 0.0009761818 4.476483e-01 5.890261e-01 14 100141794 100142235 442 + 1.267 1.196 -0.252
ENSG00000196405 E050 703.9881375 0.0002565254 2.220735e-11 4.372392e-10 14 100143701 100143890 190 + 2.681 2.792 0.369
ENSG00000196405 E051 561.2212666 0.0016347897 1.698288e-08 2.006333e-07 14 100143891 100144013 123 + 2.556 2.701 0.483
ENSG00000196405 E052 512.7495837 0.0023475672 7.163438e-12 1.527225e-10 14 100144014 100144400 387 + 2.468 2.672 0.680