ENSG00000196376

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000360388 ENSG00000196376 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC35F1 protein_coding protein_coding 8.294895 6.856712 11.44341 0.3602359 0.135622 0.7380865 0.5987144 0.000000 1.037570 0.00000000 0.5763412 6.7109033 0.053775 0.000 0.08993333 0.08993333 0.24739732 0.02158963 FALSE TRUE
ENST00000621341 ENSG00000196376 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC35F1 protein_coding protein_coding 8.294895 6.856712 11.44341 0.3602359 0.135622 0.7380865 5.5286496 5.003634 7.126353 0.31245683 0.2053095 0.5093304 0.680550 0.729 0.62263333 -0.10636667 0.02158963 0.02158963 FALSE TRUE
MSTRG.28873.1 ENSG00000196376 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC35F1 protein_coding   8.294895 6.856712 11.44341 0.3602359 0.135622 0.7380865 2.1675313 1.853078 3.279491 0.04942586 0.4632272 0.8201765 0.265675 0.271 0.28740000 0.01640000 0.94609672 0.02158963 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196376 E001 3.8903873 0.1439790760 5.965533e-01 7.184785e-01 6 117907220 117907263 44 + 0.669 0.690 0.085
ENSG00000196376 E002 44.3262329 0.0006022751 2.906815e-11 5.611244e-10 6 117907264 117907899 636 + 1.704 1.461 -0.829
ENSG00000196376 E003 0.2966881 0.0270030970 3.685118e-01   6 118154442 118154444 3 + 0.156 0.000 -8.854
ENSG00000196376 E004 66.5204917 0.0250986609 1.385568e-04 6.972447e-04 6 118154445 118154620 176 + 1.864 1.671 -0.649
ENSG00000196376 E005 71.8537718 0.0163612788 9.215283e-04 3.690457e-03 6 118235509 118235636 128 + 1.872 1.768 -0.352
ENSG00000196376 E006 68.5741238 0.0005128932 5.642211e-06 3.968737e-05 6 118266995 118267154 160 + 1.840 1.783 -0.191
ENSG00000196376 E007 61.0006005 0.0004032485 2.652796e-05 1.598436e-04 6 118275459 118275615 157 + 1.789 1.739 -0.171
ENSG00000196376 E008 33.0539820 0.0031523379 3.154146e-04 1.442575e-03 6 118277494 118277546 53 + 1.544 1.438 -0.362
ENSG00000196376 E009 53.0495137 0.0005260937 2.255665e-05 1.381779e-04 6 118285184 118285338 155 + 1.736 1.668 -0.232
ENSG00000196376 E010 69.7037561 0.0004095268 2.057087e-08 2.392761e-07 6 118314028 118314253 226 + 1.864 1.762 -0.344
ENSG00000196376 E011 136.2424552 0.0008116604 1.923076e-07 1.848970e-06 6 118314254 118315135 882 + 2.125 2.101 -0.078
ENSG00000196376 E012 689.1869810 0.0071354762 5.916607e-19 3.346920e-17 6 118315136 118317676 2541 + 2.672 3.006 1.111