ENSG00000196363

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000358625 ENSG00000196363 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR5 protein_coding protein_coding 71.53809 90.20514 64.38702 1.022243 1.385326 -0.4863756 55.58845 48.74987 62.1841983 8.244652 0.9958727 0.3510856 0.7823250 0.5402000 0.966133333 0.4259333 9.354605e-08 9.354605e-08 FALSE TRUE
MSTRG.33584.12 ENSG00000196363 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR5 protein_coding   71.53809 90.20514 64.38702 1.022243 1.385326 -0.4863756 13.74334 39.15376 0.4634587 8.483320 0.4634587 -6.3701369 0.1862458 0.4343333 0.006933333 -0.4274000 2.046680e-03 9.354605e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196363 E001 0.6986918 1.675720e-02 5.319361e-01 6.643260e-01 9 134135130 134135224 95 + 0.142 0.261 1.099
ENSG00000196363 E002 1.1373304 1.175684e-02 8.826464e-01 9.288829e-01 9 134135225 134135364 140 + 0.334 0.322 -0.072
ENSG00000196363 E003 0.4031496 2.474914e-02 3.287987e-01 4.722869e-01 9 134135365 134135374 10 + 0.000 0.191 11.729
ENSG00000196363 E004 0.4031496 2.474914e-02 3.287987e-01 4.722869e-01 9 134135375 134135376 2 + 0.000 0.191 11.729
ENSG00000196363 E005 0.4031496 2.474914e-02 3.287987e-01 4.722869e-01 9 134135377 134135377 1 + 0.000 0.191 11.729
ENSG00000196363 E006 2.3971316 6.988693e-03 4.636249e-01 6.033933e-01 9 134135378 134135409 32 + 0.568 0.466 -0.485
ENSG00000196363 E007 5.6454275 2.955589e-03 6.383616e-01 7.520034e-01 9 134135410 134135441 32 + 0.831 0.794 -0.146
ENSG00000196363 E008 11.3908734 1.713789e-03 9.320067e-01 9.611643e-01 9 134135442 134135622 181 + 1.071 1.089 0.063
ENSG00000196363 E009 9.2777429 1.895552e-03 6.982862e-01 7.982760e-01 9 134135623 134135678 56 + 0.959 1.026 0.249
ENSG00000196363 E010 14.3876963 1.583063e-03 5.889563e-01 7.120903e-01 9 134135679 134135842 164 + 1.183 1.161 -0.081
ENSG00000196363 E011 1.1718769 4.726360e-02 5.074363e-04 2.188107e-03 9 134136082 134136091 10 + 0.611 0.000 -14.584
ENSG00000196363 E012 132.4808657 7.505201e-03 7.331875e-14 2.124831e-12 9 134136092 134136200 109 + 2.301 1.936 -1.223
ENSG00000196363 E013 2.4464374 2.076210e-01 3.909985e-01 5.349415e-01 9 134136479 134136541 63 + 0.612 0.436 -0.840
ENSG00000196363 E014 2.9613511 2.288769e-02 1.007627e-02 2.894790e-02 9 134136542 134136590 49 + 0.779 0.377 -1.858
ENSG00000196363 E015 442.0481496 2.474018e-03 1.496566e-11 3.029383e-10 9 134139820 134139958 139 + 2.729 2.560 -0.563
ENSG00000196363 E016 583.8691263 1.647662e-03 4.061654e-06 2.949284e-05 9 134140703 134140811 109 + 2.803 2.719 -0.278
ENSG00000196363 E017 528.1235248 5.957191e-04 5.561254e-04 2.370380e-03 9 134141510 134141583 74 + 2.733 2.694 -0.129
ENSG00000196363 E018 622.8184593 5.899316e-04 2.320085e-01 3.648305e-01 9 134141949 134142038 90 + 2.777 2.782 0.018
ENSG00000196363 E019 653.6076019 1.102461e-04 2.247366e-01 3.561178e-01 9 134142333 134142422 90 + 2.795 2.805 0.034
ENSG00000196363 E020 670.8482608 1.384022e-04 8.664635e-01 9.180434e-01 9 134142636 134142719 84 + 2.793 2.824 0.101
ENSG00000196363 E021 587.4682757 1.511521e-04 4.959372e-01 6.326728e-01 9 134148288 134148343 56 + 2.731 2.770 0.128
ENSG00000196363 E022 594.9510495 9.784296e-05 1.445694e-01 2.542446e-01 9 134151983 134152029 47 + 2.757 2.763 0.020
ENSG00000196363 E023 733.3060194 9.389856e-05 1.978990e-02 5.104269e-02 9 134154466 134154541 76 + 2.854 2.851 -0.013
ENSG00000196363 E024 572.1571144 1.136246e-04 1.765830e-04 8.636407e-04 9 134155340 134155373 34 + 2.763 2.733 -0.099
ENSG00000196363 E025 650.3103520 1.153116e-04 1.019288e-03 4.025286e-03 9 134155693 134155767 75 + 2.812 2.792 -0.066
ENSG00000196363 E026 762.9345275 3.926287e-04 4.199836e-01 5.632297e-01 9 134156506 134156593 88 + 2.860 2.875 0.051
ENSG00000196363 E027 17.7259077 1.431591e-02 1.030963e-01 1.951902e-01 9 134156831 134156871 41 + 1.338 1.201 -0.482
ENSG00000196363 E028 4354.8390283 1.319820e-03 6.024454e-13 1.532172e-11 9 134157893 134159968 2076 + 3.546 3.664 0.393