ENSG00000196214

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000439461 ENSG00000196214 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF766 protein_coding protein_coding 2.642009 0.9668582 4.009907 0.1697149 0.2926259 2.040941 0.6915161 0.5240216 0.7635322 0.06805798 0.06597827 0.5345633 0.3514375 0.6029333 0.19086667 -0.4120667 0.0936297720 0.0003114586 FALSE TRUE
ENST00000593703 ENSG00000196214 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF766 protein_coding protein_coding 2.642009 0.9668582 4.009907 0.1697149 0.2926259 2.040941 0.4945625 0.0000000 1.1216398 0.00000000 0.36192517 6.8222710 0.1145167 0.0000000 0.27233333 0.2723333 0.0003114586 0.0003114586 FALSE TRUE
ENST00000599581 ENSG00000196214 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF766 protein_coding protein_coding 2.642009 0.9668582 4.009907 0.1697149 0.2926259 2.040941 1.1667868 0.3033892 1.9449625 0.16710536 0.09686486 2.6411134 0.4209167 0.2653000 0.48923333 0.2239333 0.4937883300 0.0003114586 FALSE TRUE
ENST00000601711 ENSG00000196214 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF766 protein_coding protein_coding 2.642009 0.9668582 4.009907 0.1697149 0.2926259 2.040941 0.1438273 0.1266040 0.1724629 0.06389533 0.17246295 0.4176038 0.0652875 0.1113333 0.04553333 -0.0658000 0.4893743865 0.0003114586 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196214 E001 0.4720498 0.1070800635 8.754956e-01 9.240363e-01 19 52269587 52269592 6 + 0.146 0.184 0.399
ENSG00000196214 E002 7.5119122 0.0023790568 4.511761e-01 5.921505e-01 19 52269593 52269631 39 + 0.915 0.865 -0.189
ENSG00000196214 E003 0.0000000       19 52274462 52274474 13 +      
ENSG00000196214 E004 0.0000000       19 52274475 52274513 39 +      
ENSG00000196214 E005 0.0000000       19 52274514 52274582 69 +      
ENSG00000196214 E006 0.0000000       19 52274583 52274669 87 +      
ENSG00000196214 E007 0.0000000       19 52275578 52275607 30 +      
ENSG00000196214 E008 0.4460135 0.0459546487 3.187774e-01 4.617540e-01 19 52277154 52277541 388 + 0.205 0.000 -10.041
ENSG00000196214 E009 12.9528184 0.0014748368 1.800846e-02 4.715153e-02 19 52282111 52282237 127 + 1.172 0.975 -0.714
ENSG00000196214 E010 0.1472490 0.0435621804 1.000000e+00   19 52282238 52282574 337 + 0.079 0.000 -8.506
ENSG00000196214 E011 13.1252620 0.0014004450 3.070953e-03 1.047483e-02 19 52283285 52283413 129 + 1.189 0.924 -0.967
ENSG00000196214 E012 0.5181333 0.0201713352 8.944948e-01 9.366069e-01 19 52288042 52288115 74 + 0.147 0.184 0.397
ENSG00000196214 E013 69.9865377 0.0005047382 3.606258e-10 5.792732e-09 19 52290066 52291124 1059 + 1.874 1.662 -0.718
ENSG00000196214 E014 63.5619147 0.0004235887 1.560169e-02 4.185054e-02 19 52291125 52292148 1024 + 1.727 1.871 0.486
ENSG00000196214 E015 61.2656229 0.0202827651 3.038147e-05 1.805380e-04 19 52292149 52296046 3898 + 1.635 1.954 1.078