ENSG00000196177

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000358776 ENSG00000196177 HEK293_OSMI2_2hA HEK293_TMG_2hB ACADSB protein_coding protein_coding 8.771126 2.602576 15.45142 0.2073016 1.24183 2.565128 5.73304082 2.2909346 9.8025217 0.19753418 0.70341063 2.092404 0.730545833 0.88396667 0.6363 -0.24766667 4.297802e-02 9.548097e-13 FALSE TRUE
ENST00000368869 ENSG00000196177 HEK293_OSMI2_2hA HEK293_TMG_2hB ACADSB protein_coding protein_coding 8.771126 2.602576 15.45142 0.2073016 1.24183 2.565128 0.02569035 0.2055228 0.0000000 0.20552282 0.00000000 -4.429769 0.009658333 0.07726667 0.0000 -0.07726667 4.548611e-01 9.548097e-13 FALSE TRUE
ENST00000411816 ENSG00000196177 HEK293_OSMI2_2hA HEK293_TMG_2hB ACADSB protein_coding processed_transcript 8.771126 2.602576 15.45142 0.2073016 1.24183 2.565128 2.15320795 0.0000000 4.2800499 0.00000000 0.81738789 8.744851 0.139150000 0.00000000 0.2720 0.27200000 9.548097e-13 9.548097e-13 FALSE FALSE
ENST00000541070 ENSG00000196177 HEK293_OSMI2_2hA HEK293_TMG_2hB ACADSB protein_coding retained_intron 8.771126 2.602576 15.45142 0.2073016 1.24183 2.565128 0.26897570 0.1061190 0.2691848 0.07389652 0.09366896 1.265616 0.060629167 0.03876667 0.0179 -0.02086667 8.134617e-01 9.548097e-13   FALSE
MSTRG.4766.1 ENSG00000196177 HEK293_OSMI2_2hA HEK293_TMG_2hB ACADSB protein_coding   8.771126 2.602576 15.45142 0.2073016 1.24183 2.565128 0.41729158 0.0000000 0.7717032 0.00000000 0.27874952 6.288549 0.042925000 0.00000000 0.0508 0.05080000 1.068071e-02 9.548097e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196177 E001 0.5943067 0.0268665484 7.763610e-01 8.559829e-01 10 123008974 123008978 5 + 0.168 0.223 0.498
ENSG00000196177 E002 1.4863456 0.0509953247 4.689525e-01 6.082878e-01 10 123008979 123008988 10 + 0.324 0.476 0.849
ENSG00000196177 E003 2.0817981 0.0337289921 8.713021e-01 9.212225e-01 10 123008989 123009005 17 + 0.438 0.477 0.201
ENSG00000196177 E004 17.7206126 0.0118546637 1.007015e-01 1.915326e-01 10 123009006 123009067 62 + 1.233 1.044 -0.679
ENSG00000196177 E005 16.9821654 0.0171528296 6.482634e-02 1.347564e-01 10 123009068 123009071 4 + 1.221 0.988 -0.839
ENSG00000196177 E006 0.0000000       10 123027534 123027541 8 +      
ENSG00000196177 E007 0.8856842 0.0908078806 2.581975e-01 3.954176e-01 10 123027542 123027592 51 + 0.289 0.001 -9.674
ENSG00000196177 E008 53.3481376 0.0037905662 9.558149e-03 2.767989e-02 10 123034356 123034515 160 + 1.689 1.513 -0.600
ENSG00000196177 E009 56.9028424 0.0036084574 2.005895e-03 7.244514e-03 10 123037747 123037847 101 + 1.720 1.513 -0.705
ENSG00000196177 E010 94.7740184 0.0003424022 9.502189e-05 4.986320e-04 10 123040466 123040672 207 + 1.936 1.758 -0.599
ENSG00000196177 E011 78.6671835 0.0003802467 4.357826e-02 9.760555e-02 10 123041209 123041379 171 + 1.842 1.748 -0.319
ENSG00000196177 E012 60.5138505 0.0003866211 2.074647e-02 5.305246e-02 10 123043046 123043171 126 + 1.736 1.608 -0.433
ENSG00000196177 E013 43.8184372 0.0034266814 9.474635e-03 2.747464e-02 10 123044393 123044485 93 + 1.611 1.422 -0.646
ENSG00000196177 E014 51.7548510 0.0005282140 2.348948e-01 3.682291e-01 10 123047209 123047298 90 + 1.660 1.593 -0.227
ENSG00000196177 E015 69.6579344 0.0003728906 6.923191e-02 1.420979e-01 10 123051049 123051186 138 + 1.789 1.698 -0.306
ENSG00000196177 E016 4.0509916 0.0386510871 6.488397e-01 7.602683e-01 10 123052761 123053060 300 + 0.651 0.567 -0.371
ENSG00000196177 E017 77.2338286 0.0003599626 8.241686e-02 1.633164e-01 10 123053061 123053160 100 + 1.829 1.748 -0.275
ENSG00000196177 E018 728.3077437 0.0002816640 3.543707e-22 2.870221e-20 10 123053695 123058290 4596 + 2.756 2.866 0.366