ENSG00000196159

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000335110 ENSG00000196159 HEK293_OSMI2_2hA HEK293_TMG_2hB FAT4 protein_coding protein_coding 0.4111578 0.2285551 0.4181953 0.03284408 0.08758607 0.8439465 0.029838861 0.00000000 0.04322617 0.000000000 0.043226173 2.4121358 0.0506500 0.0000000 0.11056667 0.11056667 0.88858147 0.01127375 FALSE TRUE
ENST00000394329 ENSG00000196159 HEK293_OSMI2_2hA HEK293_TMG_2hB FAT4 protein_coding protein_coding 0.4111578 0.2285551 0.4181953 0.03284408 0.08758607 0.8439465 0.190278918 0.03656749 0.21536514 0.027607690 0.006234568 2.2748692 0.4076417 0.1874333 0.56706667 0.37963333 0.25697688 0.01127375 FALSE TRUE
ENST00000509444 ENSG00000196159 HEK293_OSMI2_2hA HEK293_TMG_2hB FAT4 protein_coding retained_intron 0.4111578 0.2285551 0.4181953 0.03284408 0.08758607 0.8439465 0.009110687 0.00000000 0.07288549 0.000000000 0.072885493 3.0511196 0.0156625 0.0000000 0.12530000 0.12530000 0.87105391 0.01127375   FALSE
ENST00000674496 ENSG00000196159 HEK293_OSMI2_2hA HEK293_TMG_2hB FAT4 protein_coding protein_coding 0.4111578 0.2285551 0.4181953 0.03284408 0.08758607 0.8439465 0.154260329 0.16782597 0.05718466 0.045029897 0.034592129 -1.4042624 0.4538625 0.7110000 0.13000000 -0.58100000 0.01127375 0.01127375 FALSE TRUE
ENST00000678072 ENSG00000196159 HEK293_OSMI2_2hA HEK293_TMG_2hB FAT4 protein_coding processed_transcript 0.4111578 0.2285551 0.4181953 0.03284408 0.08758607 0.8439465 0.027669038 0.02416162 0.02953382 0.008760289 0.010453423 0.2107112 0.0721750 0.1015667 0.06703333 -0.03453333 0.92581958 0.01127375 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000196159 E001 0.1451727 0.042831819 7.836419e-01   4 125314918 125314954 37 + 0.098 0.000 -8.814
ENSG00000196159 E002 1.8028145 0.008649789 4.911046e-01 6.284120e-01 4 125314955 125315977 1023 + 0.480 0.354 -0.684
ENSG00000196159 E003 7.8404419 0.195126216 1.030312e-01 1.950901e-01 4 125316400 125318805 2406 + 1.047 0.725 -1.236
ENSG00000196159 E004 11.2794819 0.002036528 1.242686e-03 4.785413e-03 4 125318806 125321586 2781 + 1.202 0.862 -1.248
ENSG00000196159 E005 1.1115096 0.021003497 1.874904e-01 3.105692e-01 4 125338033 125341803 3771 + 0.401 0.152 -1.854
ENSG00000196159 E006 0.0000000       4 125393936 125394004 69 +      
ENSG00000196159 E007 0.9859167 0.015459749 8.486614e-01 9.060426e-01 4 125398784 125398915 132 + 0.303 0.265 -0.269
ENSG00000196159 E008 2.0337255 0.007544274 8.378286e-02 1.654703e-01 4 125406880 125407141 262 + 0.578 0.264 -1.730
ENSG00000196159 E009 2.9131807 0.005383626 8.468627e-03 2.495732e-02 4 125408444 125408794 351 + 0.724 0.264 -2.358
ENSG00000196159 E010 5.4523660 0.003854878 2.753887e-02 6.708874e-02 4 125414884 125415806 923 + 0.907 0.595 -1.271
ENSG00000196159 E011 1.7265419 0.009170427 1.730406e-01 2.921436e-01 4 125416448 125416622 175 + 0.515 0.264 -1.440
ENSG00000196159 E012 1.3599240 0.010322583 7.935382e-02 1.584968e-01 4 125434245 125434425 181 + 0.480 0.152 -2.271
ENSG00000196159 E013 0.0000000       4 125446111 125446292 182 +      
ENSG00000196159 E014 0.7406253 0.015435426 6.185337e-02 1.297380e-01 4 125446293 125446298 6 + 0.355 0.000 -12.834
ENSG00000196159 E015 2.8831055 0.021405177 1.559559e-03 5.827949e-03 4 125446299 125446543 245 + 0.744 0.152 -3.439
ENSG00000196159 E016 2.3670459 0.008044783 1.537808e-01 2.668082e-01 4 125448461 125448578 118 + 0.606 0.354 -1.270
ENSG00000196159 E017 25.5356887 0.001011270 5.296567e-01 6.623970e-01 4 125448579 125452810 4232 + 1.436 1.389 -0.164
ENSG00000196159 E018 2.4272083 0.006956933 4.569511e-01 5.973075e-01 4 125463563 125463667 105 + 0.480 0.595 0.536
ENSG00000196159 E019 3.1181665 0.005571401 2.848094e-02 6.897575e-02 4 125468512 125468819 308 + 0.724 0.354 -1.773
ENSG00000196159 E020 2.2355908 0.081139288 9.368843e-02 1.808851e-01 4 125476171 125476256 86 + 0.606 0.267 -1.843
ENSG00000196159 E021 3.0021361 0.007478900 7.354154e-01 8.261910e-01 4 125477155 125477334 180 + 0.606 0.546 -0.269
ENSG00000196159 E022 0.0000000       4 125477335 125477393 59 +      
ENSG00000196159 E023 2.1672674 0.007599144 5.083769e-01 6.438039e-01 4 125479741 125479865 125 + 0.442 0.546 0.508
ENSG00000196159 E024 4.1309587 0.003913895 2.682199e-01 4.066606e-01 4 125481521 125481738 218 + 0.632 0.780 0.614
ENSG00000196159 E025 5.8164090 0.003994947 1.651558e-02 4.387881e-02 4 125487345 125487606 262 + 0.681 0.972 1.144
ENSG00000196159 E026 21.2761103 0.004904333 2.751099e-06 2.077106e-05 4 125489901 125491768 1868 + 1.166 1.500 1.165
ENSG00000196159 E027 14.0352001 0.011771253 1.653390e-08 1.957563e-07 4 125491769 125492932 1164 + 0.849 1.398 1.984